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公开(公告)号:US06399700B2
公开(公告)日:2002-06-04
申请号:US09817887
申请日:2001-03-26
IPC分类号: C08J524
CPC分类号: A61K47/48 , A61K47/50 , A61K47/56 , A61K47/641 , C08G81/00 , C08G81/024 , C08J5/24
摘要: Synthetic comb copolymers which elicit controlled cellular response, methods of applying these polymers to various surfaces, and methods of using the polymers for modifying biomaterial surfaces, in tissue engineering applications and as drug delivery devices are provided. The comb copolymers are comprised of hydrophobic polymer backbones and hydrophilic, non-cell binding side chains which can be end-capped with cell-signaling ligands that guide cellular response. By mixing non-cell binding combs with ligand-bearing combs, the surface concentration and spatial distribution of one or more types of ligands, including adhesion peptides and growth factors, can be tuned on a surface to achieve desired cellular response. In one embodiment, the combs are used as stabilizing agents for dispersion polymerization of latexes. The comb-stabilized latexes can be applied to substrates by standard coating operations to create a bioregulating surface, or used as drug delivery agents. In another embodiment, the combs can be blended in small quantities to a hydrophobic matrix polymer and processed to affect the surface segregation of the comb. The comb copolymers are formed in one embodiment by providing a biodegradable polyester backbone that includes reactive groups, and reacting the reactive groups in the backbone with reactive chain ends on a low molecular weight hydrophilic polymer. In another embodiment, non-biodegradable comb copolymers are formed by free radical synthesis of a hydrophobic monomer and a hydrophilic macromonomer. In all of the above embodiments, a portion of the hydrophilic polymer side chains can be covalently coupled to cell-signaling ligands such as adhesion peptides or growth factors to control cellular response.
摘要翻译: 提供引起受控细胞反应的合成梳状共聚物,将这些聚合物应用于各种表面的方法,以及使用聚合物改性生物材料表面的方法,在组织工程应用中以及作为药物递送装置。 梳状共聚物由疏水性聚合物主链和亲水性非细胞结合侧链组成,其可以用引导细胞反应的细胞信号传导配体封端。 通过将非细胞结合梳与含配体的梳子混合,可以在表面上调节一种或多种类型的配体(包括粘附肽和生长因子)的表面浓度和空间分布,以实现所需的细胞反应。 在一个实施方案中,梳子用作胶乳分散聚合的稳定剂。 梳状稳定的胶乳可以通过标准涂布操作施加到基底上以产生生物调节表面,或用作药物递送剂。 在另一个实施方案中,梳子可以少量混合到疏水性基质聚合物中并进行处理以影响梳子的表面分离。 梳状共聚物在一个实施方案中通过提供包含反应性基团的可生物降解的聚酯主链,并使主链中的反应性基团与低分子量亲水聚合物上的反应性链端端反应形成。 在另一个实施方案中,通过自由基合成疏水性单体和亲水性大分子单体形成不可生物降解的梳状共聚物。 在所有上述实施方案中,一部分亲水性聚合物侧链可共价偶联至细胞信号传导配体如粘附肽或生长因子以控制细胞反应。
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公开(公告)号:US06207749B1
公开(公告)日:2001-03-27
申请号:US09634095
申请日:2000-08-08
IPC分类号: C08J524
CPC分类号: A61K47/48 , A61K47/50 , A61K47/56 , A61K47/641 , C08G81/00 , C08G81/024 , C08J5/24
摘要: Synthetic comb copolymers which elicit controlled cellular response, methods of applying these polymers to various surfaces, and methods of using the polymers for modifying biomaterial surfaces, in tissue engineering applications and as drug delivery devices are provided. The comb copolymers are comprised of hydrophobic polymer backbones and hydrophilic, non-cell binding side chains which can be end-capped with cell-signaling ligands that guide cellular response. By mixing non-cell binding combs with ligand-bearing combs, the surface concentration and spatial distribution of one or more types of ligands, including adhesion peptides and growth factors, can be tuned on a surface to achieve desired cellular response. In one embodiment, the combs are used as stabilizing agents for dispersion polymerization of latexes. The comb-stabilized latexes can be applied to substrates by standard coating operations to create a bioregulating surface, or used as drug delivery agents. In another embodiment, the combs can be blended in small quantities to a hydrophobic matrix polymer and processed to affect the surface segregation of the comb. The comb copolymers are formed in one embodiment by providing a biodegradable polyester backbone that includes reactive groups, and reacting the reactive groups in the backbone with reactive chain ends on a low molecular weight hydrophilic polymer. In another embodiment, non-biodegradable comb copolymers are formed by free radical synthesis of a hydrophobic monomer and a hydrophilic macromonomer. In all of the above embodiments, a portion of the hydrophilic polymer side chains can be covalently coupled to cell-signaling ligands such as adhesion peptides or growth factors to control cellular response.
摘要翻译: 提供引起受控细胞反应的合成梳状共聚物,将这些聚合物应用于各种表面的方法,以及使用聚合物改性生物材料表面的方法,在组织工程应用中以及作为药物递送装置。 梳状共聚物由疏水性聚合物主链和亲水性非细胞结合侧链组成,其可以用引导细胞反应的细胞信号传导配体封端。 通过将非细胞结合梳与含配体的梳子混合,可以在表面上调节一种或多种类型的配体(包括粘附肽和生长因子)的表面浓度和空间分布,以实现所需的细胞反应。 在一个实施方案中,梳子用作胶乳分散聚合的稳定剂。 梳状稳定的胶乳可以通过标准涂布操作施加到基底上以产生生物调节表面,或用作药物递送剂。 在另一个实施方案中,梳子可以少量混合到疏水性基质聚合物中并进行处理以影响梳子的表面分离。 梳状共聚物在一个实施方案中通过提供包含反应性基团的可生物降解的聚酯主链,并使主链中的反应性基团与低分子量亲水聚合物上的反应性链端端反应形成。 在另一个实施方案中,通过自由基合成疏水性单体和亲水性大分子单体形成不可生物降解的梳状共聚物。 在所有上述实施方案中,一部分亲水性聚合物侧链可共价偶联至细胞信号传导配体如粘附肽或生长因子以控制细胞反应。
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公开(公告)号:US6150459A
公开(公告)日:2000-11-21
申请号:US290140
申请日:1999-04-13
CPC分类号: C08G81/024 , A61K47/48169 , A61K47/48253 , C08G81/00
摘要: Synthetic comb copolymers which elicit controlled cellular response, methods of applying these polymers to various surfaces, and methods of using the polymers for modifying biomaterial surfaces, in tissue engineering applications and as drug delivery devices are provided. The comb copolymers are comprised of hydrophobic polymer backbones and hydrophilic, non-cell binding side chains which can be end-capped with cell-signaling ligands that guide cellular response. By mixing non-cell binding combs with ligand-bearing combs, the surface concentration and spatial distribution of one or more types of ligands, including adhesion peptides and growth factors, can be tuned on a surface to achieve desired cellular response. In one embodiment, the combs are used as stabilizing agents for dispersion polymerization of latexes. The comb-stabilized latexes can be applied to substrates by standard coating operations to create a bioregulating surface, or used as drug delivery agents. In another embodiment, the combs can be blended in small quantities to a hydrophobic matrix polymer and processed to affect the surface segregation of the comb. The comb copolymers are formed in one embodiment by providing a biodegradable polyester backbone that includes reactive groups, and reacting the reactive groups in the backbone with reactive chain ends on a low molecular weight hydrophilic polymer. In another embodiment, non-biodegradable comb copolymers are formed by free radical synthesis of a hydrophobic monomer and a hydrophilic macromonomer. In all of the above embodiments, a portion of the hydrophilic polymer side chains can be covalently coupled to cell-signaling ligands such as adhesion peptides or growth factors to control cellular response.
摘要翻译: 提供引起受控细胞反应的合成梳状共聚物,将这些聚合物应用于各种表面的方法,以及使用聚合物改性生物材料表面的方法,在组织工程应用中以及作为药物递送装置。 梳状共聚物由疏水性聚合物主链和亲水性非细胞结合侧链组成,其可以用引导细胞反应的细胞信号传导配体封端。 通过将非细胞结合梳与含配体的梳子混合,可以在表面上调节一种或多种类型的配体(包括粘附肽和生长因子)的表面浓度和空间分布,以实现所需的细胞反应。 在一个实施方案中,梳子用作胶乳分散聚合的稳定剂。 梳状稳定的胶乳可以通过标准涂布操作施加到基底上以产生生物调节表面,或用作药物递送剂。 在另一个实施方案中,梳子可以少量混合到疏水性基质聚合物中并进行处理以影响梳子的表面分离。 梳状共聚物在一个实施方案中通过提供包含反应性基团的可生物降解的聚酯主链,并使主链中的反应性基团与低分子量亲水聚合物上的反应性链端端反应形成。 在另一个实施方案中,通过自由基合成疏水性单体和亲水性大分子单体形成不可生物降解的梳状共聚物。 在所有上述实施方案中,一部分亲水性聚合物侧链可共价偶联至细胞信号传导配体如粘附肽或生长因子以控制细胞反应。
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14.
公开(公告)号:US08562836B2
公开(公告)日:2013-10-22
申请号:US12957064
申请日:2010-11-30
IPC分类号: B01D61/14 , B01D69/02 , B01D71/34 , B01D71/78 , C08F259/08
CPC分类号: B01D67/0018 , B01D69/02 , B01D69/141 , B01D71/30 , B01D71/34 , B01D71/40 , B01D71/52 , B01D71/78 , B01D2323/02 , B01D2325/28 , B01D2325/32 , C08G81/00 , C08G81/021
摘要: The present invention relates to synthetic methods for grafting hydrophilic chains onto polymers, particularly hydrophobic polymers such as poly(vinyl chloride) (PVC), poly(vinylidene fluoride) (PVDF), and chlorinated polypropylene (cPP). Resulting polymers include comb polymers which can have a microphase-separated structure of hydrophilic domains provided by the hydrophilic chains. Articles prepared from these comb polymers, particularly derived from PVDF, include membranes for water filtration in which the hydrophilic domains provide a pathway for water transport. PVC can be plasticized by grafting the PVC with hydrophilic chains. In addition, such articles, particularly articles having biomedical applications, can display anti-thrombogenic properties.
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