公开(公告)号：US20110064618A1

公开(公告)日：2011-03-17

申请号：US12942356

申请日：2010-11-09

申请人： Wolfgang Petrich ,  Claudia Gaessler-Dietsche ,  Carina Horn ,  Kai Hebestreit ,  Jean-Michel Asfour ,  Klemens Bardelang ,  Gerrit Kocherscheidt

发明人： Wolfgang Petrich ,  Claudia Gaessler-Dietsche ,  Carina Horn ,  Kai Hebestreit ,  Jean-Michel Asfour ,  Klemens Bardelang ,  Gerrit Kocherscheidt

IPC分类号： G01N21/64 ,  G01J1/58

CPC分类号： C12Q1/008 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/54 ,  G01N33/52 ,  G01N33/66 ,  Y10T436/144444

摘要： The invention relates to processes and devices for detecting an analyte in a sample by fluorescence measurement of a fluorophore, wherein the detection medium which contains a fluorophore or a precursor of the fluorophore is admixed with an absorber whose absorbance spectrum superimposes the fluorescence excitation range of the fluorophore. The system consisting of the fluorophore and the absorber, which is produced in the detection medium, has an altered effective fluorescence excitation range with an altered fluorescence excitation maximum. Illumination with fluorescence excitation light can take place within the range of this altered excitation maximum. The measured signal obtained from determining fluorescence emission exhibits only low dependence on the wavelength of the excitation light.

摘要翻译： 本发明涉及通过荧光团的荧光测定来检测样品中分析物的方法和装置，其中将含有荧光团或荧光团前体的检测介质与吸收光谱与吸收光谱的荧光激发范围相加 荧光团。 由荧光团和在检测介质中产生的吸收体组成的系统具有改变的有效荧光激发范围，具有改变的荧光激发最大值。 荧光激发光的照明可以在这种改变的激发最大值的范围内进行。 从确定荧光发射获得的测量信号仅对激发光的波长的依赖性低。