公开(公告)号：US20220186310A1

公开(公告)日：2022-06-16

申请号：US17511046

申请日：2021-10-26

Applicant: Element Biosciences, Inc.

Inventor： Sinan Arslan ,  Molly Min HE ,  Matthew KELLINGER ,  Jake LEVIEUX ,  Michael PREVITE ,  Junhua ZHAO ,  Su ZHANG ,  Tyler LOPEZ

IPC: C12Q1/6874 ,  G01N33/53 ,  G01N33/58

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20210139884A1

公开(公告)日：2021-05-13

申请号：US17120030

申请日：2020-12-11

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Matthew KELLINGER ,  Molly HE ,  Tyler LOPEZ

IPC: C12N15/10 ,  C12N9/12

Abstract: Provided herein are compositions and methods for the incorporation of unnatural nucleotides using mutant polymerases, such as reverse transcriptases. Further provided herein are methods of detection and sequencing of polynucleotide sequences. In some aspects, the compositions and methods are used enhance the efficiency and speed of detecting nucleotide bases. The methods and compositions described herein may further reduce time, cost, or scale of devices for next generation sequencing platforms.