公开(公告)号：US5889285A

公开(公告)日：1999-03-30

申请号：US609614

申请日：1996-03-01

申请人： Mitsushi Murata ,  Tsunenori Takahashi ,  Yukio Kato

发明人： Mitsushi Murata ,  Tsunenori Takahashi ,  Yukio Kato

IPC分类号： C08F2/20 ,  B01F17/00 ,  C08F2/18

CPC分类号： B01F17/005 ,  B01F17/0007 ,  B01F17/0028 ,  B01F17/0042 ,  B01F17/0057 ,  B01F17/0064 ,  B01F17/0085 ,  C08F2/18

摘要： A suspending agent-containing slurry is disclosed which comprises a dispersant and a particulate suspending agent having a particle size distribution in which the particle diameter at weight-cumulative 95%, from the minimum particle size, is 1 .mu.m or smaller. A method for efficiently producing the suspending agent-containing slurry is also disclosed. A process for suspension polymerization by use of the suspending agent-containing slurry is further disclosed. The slurry not only has such an excellent suspending ability in suspension polymerization that it enables the production of vinyl polymer beads having a narrow bead size distribution, but also is capable of retaining this ability over long and of exhibiting the same ability even when it has a high concentration. The suspension polymerization process enables vinyl polymer beads having a narrow bead size distribution to be produced efficiently due to the use of the slurry.

摘要翻译： 公开了一种含悬浮剂的浆料，其包含分散剂和粒度分布的颗粒状悬浮剂，其中粒径从最小颗粒尺寸的重量累积95％为1μm或更小。 还公开了一种有效生产含悬浮剂的浆料的方法。 进一步公开了通过使用含悬浮剂的浆料进行悬浮聚合的方法。 该浆料不仅在悬浮聚合中具有优异的悬浮能力，能够制造具有窄珠粒度分布的乙烯基聚合物珠粒，而且能够长时间保持该能力，并且即使具有相同的能力也能表现出相同的能力 高浓度 悬浮聚合方法使得能够由于使用浆料而有效地制备具有窄珠粒度分布的乙烯基聚合物珠粒。

公开(公告)号：US5607920A

公开(公告)日：1997-03-04

申请号：US278617

申请日：1994-07-21

申请人： Yukio Kato ,  Haiou Pan ,  Kazuyuki Doi

发明人： Yukio Kato ,  Haiou Pan ,  Kazuyuki Doi

IPC分类号： A61K38/00 ,  C07K14/51 ,  C07K1/00 ,  C12N9/00

CPC分类号： C07K14/51 ,  A61K38/00 ,  Y10S530/84

摘要： The invention is directed to purified and isolated concanavalin A-binding proteins from chondrocytes that are not present in dedifferentiated cells from chondrocytes. The invention is also directed to a purified and isolated chondrocyte membrane protein, (CMP), which is a concanavalin A-binding protein, with a molecular weight of about 76 kD in SDS-PAGE. After treatment with endoglycosidase, CMP has an apparent molecular weight of about 67 kD. The N-terminal amino acid sequence and several internal amino acid sequences are given for CMP. These proteins can be used in assays, methods, or treatments involving differentiation of chondrocytes and the control of cartilaginous osteogenesis.

摘要翻译： 本发明涉及来自软骨细胞的不存在于来自软骨细胞的去分化细胞中的纯化和分离的伴刀豆球蛋白A-结合蛋白。 本发明还涉及在SDS-PAGE中分子量约76kD的纯化和分离的软骨细胞膜蛋白（CMP），其是刀豆素A结合蛋白，分子量约为76kD。 用糖苷内切酶处理后，CMP的表观分子量约为67kD。 给出了N末端氨基酸序列和几个内部氨基酸序列。 这些蛋白质可用于测定，方法或涉及软骨细胞分化和控制软骨成骨的治疗。

公开(公告)号：US4972572A

公开(公告)日：1990-11-27

申请号：US929781

申请日：1986-11-13

申请人： Tatsuo Kimura ,  Yukio Kato ,  Sousuke Yamada ,  Kunio Kato ,  Masao Matsumoto ,  Shigeo Arai ,  Michio Takebe ,  Terukazu Kawarada ,  Haruo Masaki

发明人： Tatsuo Kimura ,  Yukio Kato ,  Sousuke Yamada ,  Kunio Kato ,  Masao Matsumoto ,  Shigeo Arai ,  Michio Takebe ,  Terukazu Kawarada ,  Haruo Masaki

IPC分类号： H01L21/00 ,  H05K13/00

CPC分类号： H01L21/67092 ,  H05K13/0092 ,  Y10T29/5139 ,  Y10T29/5149 ,  Y10T83/4635

摘要： An IC sheet cutting press performs a series of press cutting operations to separate ICs from an IC sheet while feeding the IC sheet stepwise. In the IC sheet cutting press, a series of press molds and an IC feed mechanism are mounted on a common die set to constitute a single press mold unit. When a different type of IC sheet is to be machined, the entire press mold unit can be exchanged with another one, so that various adjustments, e.g., adjustment of arrangement intervals of the press molds or a feed interval of the IC sheet can be omitted, and the operating efficiency of the cutting press and hence the compatibility are improved.An IC sheet processing apparatus, in which the IC sheet cutting press described aboved is combined with an automatic IC sheet loading unit, an IC lead bending press, and an IC storage unit for storing ICs after the lead bending operation, allows automatic IC sheet processing including the cutting operation of ICs from the IC sheet over a long period to time.

摘要翻译： IC片材切割机进行一系列的压制切割操作，以将IC片与IC片分离，同时逐步进给IC片。 在IC片材切割机中，将一系列压模和IC进给机构安装在共同的模具组上以构成单个压模单元。 当要加工不同类型的IC片材时，可以将整个压模模具单元更换为另一种，从而可以省略各种调整，例如调节压模的排列间隔或IC片的进给间隔 ，并且提高了切割机的操作效率，从而提高了相容性。 将上述IC片材切割机与自动IC片材装载单元，IC引线弯曲压力机，以及在引线弯曲操作之后存储IC的IC存储单元组合的IC片材处理装置允许自动IC片材加工 包括长时间从IC表切割IC的操作。

公开(公告)号：US4298961A

公开(公告)日：1981-11-03

申请号：US140839

申请日：1980-04-16

申请人： Atsuo Hotta ,  Yukio Kato

发明人： Atsuo Hotta ,  Yukio Kato

IPC分类号： G11C11/411 ,  G11C11/416 ,  H03K3/286 ,  G11C11/40

CPC分类号： H03K3/286 ,  G11C11/4116 ,  G11C11/416

摘要： An emitter junction type bipolar memory cell circuit having clamp diodes connected in parallel to collector resistances. The collector resistances are set to produce a potential difference of greater than 0.6 V or so caused by the stationary current. By so doing, it is possible to exclude any mal-function attributable to noises in the writing operation of the memory circuit.

摘要翻译： 具有与集电极电阻并联连接的钳位二极管的发射极结型双极存储单元电路。 集电极电阻被设定为产生由静止电流引起的大于0.6V左右的电位差。 通过这样做，可以排除在存储电路的写入操作中归因于噪声的任何不正常的功能。

公开(公告)号：US09234897B2

公开(公告)日：2016-01-12

申请号：US11887362

申请日：2006-03-30

申请人： Yukio Kato ,  Takeshi Kawamoto ,  Koichiro Tsuji ,  Akira Igarashi ,  Masakazu Shimizu

发明人： Yukio Kato ,  Takeshi Kawamoto ,  Koichiro Tsuji ,  Akira Igarashi ,  Masakazu Shimizu

IPC分类号： C12N5/00 ,  G01N33/68 ,  C12Q1/68 ,  G01N33/569

CPC分类号： G01N33/6845 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N33/56966

摘要： Disclosed is a method for distinguishing a mesenchymal stem cell comprising, using at least one gene selected from the genes having the nucleotide sequences indicated by the accession numbers shown in Table 1 as a distinguish marker, detecting the difference in expression of the distinguish marker between a mesenchymal stem cell and a connective tissue cell to distinguish the mesenchymal stem cell from the connective tissue cell. This method enables to distinguish an undifferentiated mesenchymal stem cell from other connective tissue cell such as fibroblasts, osteoblasts, chondrocytes and adipose cells with good accuracy. A mesenchymal stem cell given by this method or a composition comprising the mesenchymal stem sell can be used as a therapeutic for use in the regenerative medicine.

摘要翻译： 本发明公开了一种区分间充质干细胞的方法，其特征在于，使用选自具有表1所示的登录号的核苷酸序列的基因的至少一种基因作为区别标记，检测所述区别标记的表达差异 间充质干细胞和结缔组织细胞，以区分间充质干细胞与结缔组织细胞。 该方法能够以良好的精度将未分化的间充质干细胞与其他结缔组织细胞如成纤维细胞，成骨细胞，软骨细胞和脂肪细胞区分开。 通过该方法给予的间充质干细胞或包含间充质茎的组合物可用作再生医学中的治疗剂。

公开(公告)号：US20110212523A1

公开(公告)日：2011-09-01

申请号：US13127774

申请日：2009-10-22

申请人： Yukio Kato ,  Jin Chang Shao ,  Koichiro Tsuji

发明人： Yukio Kato ,  Jin Chang Shao ,  Koichiro Tsuji

IPC分类号： C12N5/077

CPC分类号： C12N5/0654 ,  C12N2500/25 ,  C12N2500/42 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/39 ,  C12N2501/40 ,  C12N2506/1353

摘要： Provided is a differentiation-inducing culture medium additive for inducing bone differentiation of at least one type of cell selected from the group consisting of a stem cell, a dental pulp cell, a periodontal ligament cell, a placenta, an amnion, and a fibroblast under a serum-free condition, and a use of the differentiation-inducing culture medium additive. The differentiation-inducing culture medium additive of the present invention for inducing differentiation of a stem cell under a serum-free condition at least contains at least one growth factor selected from the group consisting of EGF, FGF, and PDGF; dexamethasone; and β-glycerophosphate. The differentiation-inducing culture medium additive of the present invention does not require ascorbic acid 2-phosphate and ITS, which are normally essential for bone differentiation. Further, bone differentiation can be promoted by adding phospholipid.

摘要翻译： 本发明提供一种分化诱导培养基添加剂，其用于诱导选自干细胞，牙髓细胞，牙周韧带细胞，胎盘，羊膜和成纤维细胞中的至少一种类型的细胞的骨分化。 无血清条件，以及使用分化诱导培养基添加剂。 用于在无血清条件下诱导干细胞分化的本发明的分化诱导培养基添加剂至少含有至少一种选自EGF，FGF和PDGF的生长因子; 地塞米松 和甘油磷酸酯。 本发明的分化诱导培养基添加剂不需要抗坏血酸2-磷酸酯和ITS，它们通常对骨分化至关重要。 此外，通过添加磷脂可以促进骨分化。

公开(公告)号：US07969826B2

公开(公告)日：2011-06-28

申请号：US11940410

申请日：2007-11-15

申请人： Irizo Naniwa ,  Yukio Kato

发明人： Irizo Naniwa ,  Yukio Kato

IPC分类号： G11B11/00

CPC分类号： G11B5/4826 ,  G11B2005/0021

摘要： In a thermally assisted magnetic recording head support mechanism, an increase in the temperature of a heat source for supplying an optical near-field transducer with light, due to heat generation by the heat source, is checked while holding back an increase in weight of a slider and lessening transmission loss of light.The thermally assisted magnetic recording head support mechanism is made up such that a suspension 5 has one end holding a slider 4 on which a magnetic head 3 having a read element 31, write element 32, and an optical near-field transducer 34 is mounted, and the other end of the suspension 5 is held by a carriage 6. The optical block 15 provided with one light source 10, and an optical switching mechanism capable of switching a guidance destination of irradiated light from light source 10 is attached to an arm 62 or a support 61 of the carriage 6 having a wide heat release area, thereby guiding light from the optical block 15 to the optical near-field transducer 34 via an optical waveguide 14.

摘要翻译： 在热辅助磁记录头支撑机构中，检查由于热源产生热量而向光学近场换能器供给光的温度的增加，同时阻止重量增加 滑块和减轻传输损失的光。 热辅助磁记录头支撑机构被制成使得悬架5具有保持滑块4的一端，其上安装有具有读取元件31，写入元件32和光学近场换能器34的磁头3， 并且悬架5的另一端由滑架6保持。设置有一个光源10的光学块15和能够切换来自光源10的照射光的引导目的地的光学切换机构附接到臂62 或具有宽放热区域的托架6的支撑件61，从而经由光波导14将来自光学块15的光引导到光学近场换能器34。

公开(公告)号：US07891079B2

公开(公告)日：2011-02-22

申请号：US12489616

申请日：2009-06-23

申请人： Hiroshi Nozawa ,  Yukio Kato

发明人： Hiroshi Nozawa ,  Yukio Kato

IPC分类号： G11B5/127 ,  H04R31/00

CPC分类号： G11B5/4826 ,  B21D5/04 ,  Y10T29/49025 ,  Y10T29/49027 ,  Y10T29/4903 ,  Y10T29/49032 ,  Y10T29/53122 ,  Y10T29/53165

摘要： A bending apparatus of a head gimbal assembly includes a suction unit configured to attract the head gimbal assembly, a movement unit configured to move a long tail connected to a terminal part, a stopper against which the moved long tail is butted, a transmission type optical sensor configured to detect that the long tail is positioned to the stopper, a tail holder configured to hold a part of the long tail on a work table, a roller configured to compress the terminal part against the tail holder and to bend the terminal part relative to the long tail at a right angle, and a controller configured to control, based on a detection result of the transmission type optical sensor, a movement of the tail holder to the work table and a movement of the roller to the tail holder.

摘要翻译： 头万向架组件的弯曲装置包括：吸引单元，其构造成吸引头部万向架组件;移动单元，被配置为移动连接到端子部分的长尾端;移动的长尾巴对接的止动件;透射型光学 传感器，被配置为检测长尾部位于止动件上，尾部保持器构造成将长尾巴的一部分保持在工作台上;辊子，其构造成压缩终端部件抵靠尾座并弯曲终端部件相对 并且控制器被配置为基于透射型光学传感器的检测结果来控制尾座到工作台的移动以及辊到尾部保持器的移动。

公开(公告)号：US20090232773A1

公开(公告)日：2009-09-17

申请号：US11887362

申请日：2006-03-30

申请人： Yukio Kato ,  Takeshi Kawamoto ,  Koichiro Tsuji ,  Akira Igarashi ,  Masakazu Shimizu

发明人： Yukio Kato ,  Takeshi Kawamoto ,  Koichiro Tsuji ,  Akira Igarashi ,  Masakazu Shimizu

IPC分类号： A61K35/12 ,  C12Q1/68 ,  C12N5/00 ,  C40B30/04 ,  C40B40/02 ,  A61K31/7088 ,  C07K2/00 ,  C07K16/00 ,  C07H21/04

CPC分类号： G01N33/6845 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N33/56966

摘要： Disclosed is a method for distinguishing a mesenchymal stem cell comprising, using at least one gene selected from the genes having the nucleotide sequences indicated by the accession numbers shown in Table 1 as a distinguish marker, detecting the difference in expression of the distinguish marker between a mesenchymal stem cell and a connective tissue cell to distinguish the mesenchymal stem cell from the connective tissue cell. This method enables to distinguish an undifferentiated mesenchymal stem cell from other connective tissue cell such as fibroblasts, osteoblasts, chondrocytes and adipose cells with good accuracy. A mesenchymal stem cell given by this method or a composition comprising the mesenchymal stem sell can be used as a therapeutic for use in the regenerative medicine.

摘要翻译： 本发明公开了一种区分间充质干细胞的方法，其特征在于，使用选自具有表1所示的登录号的核苷酸序列的基因的至少一种基因作为区别标记，检测所述区别标记的表达差异 间充质干细胞和结缔组织细胞，以区分间充质干细胞与结缔组织细胞。 该方法能够以良好的精度将未分化的间充质干细胞与其他结缔组织细胞如成纤维细胞，成骨细胞，软骨细胞和脂肪细胞区分开。 通过该方法给予的间充质干细胞或包含间充质茎的组合物可用作再生医学中的治疗剂。

公开(公告)号：US20090221076A1

公开(公告)日：2009-09-03

申请号：US11817437

申请日：2006-02-28

申请人： Yukio Kato ,  Koichiro Tsuji ,  Katsuyuki Yamanaka

发明人： Yukio Kato ,  Koichiro Tsuji ,  Katsuyuki Yamanaka

IPC分类号： C12N5/02

CPC分类号： A61L27/18 ,  A61L27/3817 ,  A61L27/3852 ,  A61L27/56 ,  A61L2430/06 ,  C12N5/0655 ,  C12N2501/15 ,  C12N2501/39 ,  C12N2533/40 ,  C08L67/04

摘要： An objective of the present invention is to provide a production method of a sheet for regenerating a cartilage tissue, which uses a conventional sheet-shaped porous body comprising a biological absorbency synthetic high polymer, such as polylactic acid, polyglycolic acid and a copolymer of lactic acid and glycolic acid. The sheet for regenerating the cartilage tissue can differentiate chondrocytes or stem cells without culturing the cells by pressurizing such as a pressurizing culture like the conventional method, and can accumulate the cells in a supporting carrier with high efficiency. The sheet for regenerating the cartilage tissue is produced by the steps of; seeding chondrocytes or stem cells differentiating to the chondrocytes on a sheet-shaped porous body comprising a biological absorbency synthetic high polymer; taking the porous body into a culture liquid; applying acceleration of 100 to 1000 G to the porous body for a predetermined time; and culturing the porous body without applying the acceleration.

摘要翻译： 本发明的目的是提供一种用于再生软骨组织的片材的制造方法，其使用包含生物吸收性合成高分子如聚乳酸，聚乙醇酸和乳酸共聚物的常规片状多孔体 酸和乙醇酸。 用于再生软骨组织的片材可以分化软骨细胞或干细胞，而不用像常规方法那样通过加压培养细胞而不培养细胞，并且可以将细胞以高效率积累在支持载体中。 用于再生软骨组织的片材通过以下步骤制备： 在包含生物吸收性合成高分子的片状多孔体上接种与软骨细胞分化的软骨细胞或干细胞; 将多孔体吸入培养液中; 对多孔体施加100〜1000G的加速度一定时间; 并且在不施加加速度的情况下培养多孔体。