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公开(公告)号：US20080113418A1
公开(公告)日：2008-05-15
申请号：US10586109
申请日：2005-01-14
摘要： The present invention relates to processes for producing a fermentation product, such as ethanol, from milled starch-containing material comprising (a) saccharifying the milled starch-containing material with a glucoamylase having an amino acid sequence shown in SEQ ID NO: 2, or a glucoamylase being at least 70% identical thereto, at a temperature below the initial gelatinization temperature of said starch-containing material, (b) fermenting using a fermenting organism.
摘要翻译： 本发明涉及由含碾磨的含淀粉材料生产发酵产物如乙醇的方法，该方法包括：（a）用含有SEQ ID NO：2所示氨基酸序列的葡糖淀粉酶糖化含碾磨淀粉的材料，或 在低于所述含淀粉材料的初始糊化温度的温度下，（b）使用发酵生物发酵的葡糖淀粉酶与其至少70％相同。
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12.发明授权Cloud-point extraction of enzymes and polypeptides from a fermentation broth using a non-ionic surfactant 失效使用非离子表面活性剂从发酵液中提取酶和多肽的酶公开(公告)号：US07019120B2
公开(公告)日：2006-03-28
申请号：US10734431
申请日：2003-12-12
申请人： Prashant Iyer , Kishore Rane , Kevin S. Wenger , Fahd Azzabi
发明人： Prashant Iyer , Kishore Rane , Kevin S. Wenger , Fahd Azzabi
IPC分类号： C07K1/14
CPC分类号： C07K1/36 , C07K1/145 , C12N9/00 , Y10S435/816
摘要： A method of extracting a polypeptide of interest from a fermentation broth comprising: i) adjusting the pH close to pI of the polypeptide of interest; ii) adding a non-ionic surfactant with a hydrophile-lipophile balance (HLB) of 12 or lower; iii) cooling the mixture for solubilization and incubating at above cloud point for extraction; iv) phase separating at below cloud point to obtain liquid-liquid-solid fractions; and v) recovering the surfactant-rich top phase containing the polypeptide of interest.
摘要翻译： 一种从发酵液中提取目的多肽的方法，包括：i）调节pH值接近目的多肽的pI; ii）加入亲水亲油平衡（HLB）为12或更低的非离子表面活性剂; iii）冷却该混合物以溶解并在上述浊点温育以提取; iv）在低于浊点的相分离以获得液 - 液 - 固分数; 和v）回收含有目标多肽的富含表面活性剂的顶相。
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