公开(公告)号：US20190040400A1

公开(公告)日：2019-02-07

申请号：US16048920

申请日：2018-07-30

Applicant: MODERN MEADOW, INC.

Inventor： Lixin DAI ,  Julia BORDEN ,  Jeffrey NELSON ,  Kristin RUEBLING-JASS

IPC: C12N15/81 ,  C07K14/78 ,  C12P21/02

Abstract: Strains of yeast genetically engineered to produce increased amounts of non-hydroxylated collagen or hydroxylated collagen are described. A chimeric collagen DNA sequence, comprising from 10 to 40 percent or 60 to 90 percent of optimized DNA based on the total length of the chimeric collagen DN. An all-in-one vector including the DNA necessary to produce collagen, promotors, and hydroxylating enzymes is also described. Methods for producing non-hydroxylated or hydroxylated collagen are also provided.