公开(公告)号：US6107467A

公开(公告)日：2000-08-22

申请号：US924869

申请日：1997-09-05

申请人： Hans Ageland ,  Lena Nystrom ,  Josefine Persson ,  Folke Tjerneld

发明人： Hans Ageland ,  Lena Nystrom ,  Josefine Persson ,  Folke Tjerneld

IPC分类号： A61K38/00 ,  C07K20060101 ,  C07K14/775 ,  C07K1/14

CPC分类号： C07K14/775 ,  A61K38/00

摘要： The present invention relates to a process for purifying a hydrophobic or amphiphilic compound, by first mixing a starting material containing the hydrophobic or amphiphilic compound with a first polymeric material, water and at least one of a second polymeric material and a surfactant, wherein the first polymeric material and the second polymeric material and/or surfactant are immiscible in the resulting primary aqueous solution. The process further comprises maintaining the primary aqueous solution for a period of time sufficient for essentially separating the phases formed, and then removing the phase containing the main portion of the hydrophobic or amphiphilic compound and the second polymeric material and/or surfactant. The second polymeric material and/or surfactant are separated from the hydrophobic or amphiphilic compound, and subsequently recycled to the initial mixing step. The present invention further relates to a composition for use in purification of apolipoprotein A (ApoA) or apolipoprotein E (ApoE), said composition comprising a first polymeric material and a surfactant, said first polymeric material and surfactant being immiscible in the primary aqueous solution obtained after mixing with water. ApoA or ApoE produced by the inventive process can be used for the manufacture of a medicament in the treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis as well as in a method for treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis when administered in a therapeutically effective amount.

摘要翻译： 本发明涉及一种纯化疏水性或两亲性化合物的方法，首先将含有疏水性或两亲性化合物的原料与第一聚合物材料，水和至少一种第二聚合物材料和表面活性剂混合，其中第一 聚合物材料和第二聚合物材料和/或表面活性剂在所得初级水溶液中是不混溶的。 该方法还包括将初级水溶液保持一段时间，足以基本分离所形成的相，然后除去含有疏水性或两亲性化合物和第二聚合物材料和/或表面活性剂的主要部分的相。 将第二聚合物材料和/或表面活性剂与疏水性或两亲性化合物分离，随后循环至初始混合步骤。 本发明还涉及用于纯化载脂蛋白A（ApoA）或载脂蛋白E（ApoE）的组合物，所述组合物包含第一聚合物材料和表面活性剂，所述第一聚合物材料和表面活性剂在获得的主要水溶液中是不混溶的 与水混合后。 本发明方法产生的ApoA或ApoE可用于制备治疗动脉粥样硬化和心血管疾病，败血症或外周动脉粥样硬化的药物，以及用于治疗动脉粥样硬化和心血管疾病，败血症或外周动脉粥样硬化的方法 治疗有效量。