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    Labelling color, method of preparing the same and method for detecting methamphetamine with the color
    11.
    发明授权
    Labelling color, method of preparing the same and method for detecting methamphetamine with the color 失效
    标签颜色，制备方法和颜色检测甲基苯丙胺的方法

    公开(公告)号：US5488114A

    公开(公告)日：1996-01-30

    申请号：US306936

    申请日：1994-09-16

    申请人： Nobuyuki Shigetoh ,  Jipsei Miyazaki ,  Hiroshi Nakayama ,  Keiko Yugawa ,  Tadayasu Mitsumata

    发明人： Nobuyuki Shigetoh ,  Jipsei Miyazaki ,  Hiroshi Nakayama ,  Keiko Yugawa ,  Tadayasu Mitsumata

    IPC分类号： C09B23/08 ,  G01N33/58 ,  C07D209/04

    CPC分类号： C09B23/083 ,  G01N33/582 ,  Y10S435/968 ,  Y10S436/816 ,  Y10S436/901 ,  Y10T436/14

    摘要： A labelling color of pentamethine, its intermediate and a method for preparing and using the same to detect methamphetamine with high sensitivity.The invention is exemplified by pentamethine of the following Formula (1): ##STR1## wherein R.sup.1, R.sup.2, R.sup.3 and R.sup.4 each are a C.sub.1-6 alkyl group, and X is an anion.A method for using the labelling color to detect methamphetamine. The method comprises preparing a solution of the labelling color and methamphetamine-antibody in a buffer, measuring its fluorescent intensity at 660 nm in a 600 nm excited state, adding methamphetamine in a buffer to the solution, measuring its fluorescent intensity similarly, and examining the change in fluorescent intensity between both solutions.

    摘要翻译： 五甲胺的标记颜色及其中间体及其制备方法，以高灵敏度检测甲基苯丙胺。 本发明以下式（1）的五甲基胺为例：其中R1，R2，R3和R4各自为C1-6烷基，X为阴离子的式（1）。 一种使用标记颜色检测甲基苯丙胺的方法。 该方法包括在缓冲液中制备标记颜色和甲基苯丙胺抗体的溶液，在600nm激发状态下测量其在660nm处的荧光强度，向缓冲液中加入甲基苯丙胺，同样测量其荧光强度，并检查 两种溶液之间荧光强度的变化。

    Peptide fructose and protein conjugate with the same
    12.
    发明授权
    Peptide fructose and protein conjugate with the same 有权
    肽果糖和蛋白质缀合物相同

    公开(公告)号：US07109309B2

    公开(公告)日：2006-09-19

    申请号：US10312828

    申请日：2001-07-13

    申请人： Nobuyuki Shigetoh ,  Hiroshi Nakayama ,  Keiko Yugawa ,  Fumihisa Kitawaki

    发明人： Nobuyuki Shigetoh ,  Hiroshi Nakayama ,  Keiko Yugawa ,  Fumihisa Kitawaki

    IPC分类号： C07K17/10 ,  C07K16/18 ,  C12P21/08 ,  G01N33/531

    CPC分类号： C07K16/16 ,  C07K9/001 ,  C07K16/18 ,  C12P21/005 ,  Y10S436/823 ,  Y10S530/809

    摘要： It is intended to provide an antibody specific to HbA1c, antibody-producing cells capable of supplying the antibody in a stable state in the future, and a method of constructing the antibody-producing cells without any probability factors, and a method which comprises fusing mouse spleen cells, which have been sensitized with an immunogen composed of a compound containing the following structural formula (I) and a binding protein, with a myeloma-origin cell line, obtaining monoclonal antibody-producing cells by cloning, and then purifying and acquiring the monoclonal antibody produced by these cells into the culture supernatant

    摘要翻译： 旨在提供对HbA1c特异的抗体，能够在将来以稳定状态供给抗体的抗体产生细胞，以及构建没有任何概率因子的抗体产生细胞的方法，以及包括使小鼠融合的方法 已经使用由含有以下结构式（I）的化合物和结合蛋白构成的免疫原与骨髓源起始的细胞系致敏的脾细胞，通过克隆获得产生单克隆抗体的细胞，然后纯化和获得 由这些细胞产生的单克隆抗体进入培养上清液

    Immunoreaction measurement method
    13.
    发明申请
    Immunoreaction measurement method 有权
    免疫反应测定法

    公开(公告)号：US20050176063A1

    公开(公告)日：2005-08-11

    申请号：US10516067

    申请日：2003-12-09

    申请人： Akihito Kamei ,  Tatsurou Kawamura ,  Keiko Yugawa

    发明人： Akihito Kamei ,  Tatsurou Kawamura ,  Keiko Yugawa

    IPC分类号： G01N33/53 ,  G01N33/531

    CPC分类号： G01N33/5306 ,  Y10S435/961 ,  Y10S436/805

    摘要： The present invention relates to an immunoreaction measuring method for measuring an antigen or antibody contained as a subject substance in a sample, wherein the sample was mixed with at least one compound selected from the group consisting of dicarboxylic acids having a hydroxyl group, dicarboxylic acids having a double bond, straight-chain dicarboxylic acids expressed by the chemical formula (1): HOOC(CH2)nCOOH (n is an integer), and the salts of these dicarboxylic acids, and an antibody or antigen as a specifically binding substance capable of specifically binding to the subject substance, to obtain an acidic reaction solution, and then an antigen-antibody complex, generated by an antigen-antibody reaction of the subject substance with the specifically binding substance in the reaction solution was detected. This makes it possible to improve a measurement value and to relax a limitation of a measurement range due to a zone phenomenon that occurs in an antigen-excess region.

    摘要翻译： 本发明涉及一种用于测量样品中作为主体物质的抗原或抗体的免疫反应测定方法，其中将样品与选自由具有羟基的二羧酸组成的组中选择的至少一种化合物混合， 双键，由化学式（1）表示的直链二羧酸：HOOC（CH 2 CH 2）n COOH（n为整数），其盐 这些二羧酸和作为特异性结合物质的能够与本发明物质特异性结合的抗体或抗原，获得酸性反应溶液，然后将抗原 - 抗体复合物通过本发明物质的抗原 - 抗体反应与 检测反应溶液中特异性结合物质。 这使得可以改善测量值并且放松由于在抗原过量区域中发生的区域现象引起的测量范围的限制。