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    NOVEL METHODS AND CELL LINES
    12.
    发明申请
    NOVEL METHODS AND CELL LINES 审中-公开
    新的方法和细胞系

    公开(公告)号：US20110189734A1

    公开(公告)日：2011-08-04

    申请号：US12673526

    申请日：2008-08-14

    申请人： Alexander H. Taylor ,  Yuan Zhu

    发明人： Alexander H. Taylor ,  Yuan Zhu

    IPC分类号： C12P21/00 ,  C12N15/87 ,  C12N5/10

    CPC分类号： C07K16/18 ,  C07K16/00 ,  C07K16/248 ,  C12N2510/02

    摘要： This invention relates to fermentation processes for producing antibodies and other antigen binding compounds. Specifically, the present invention provides methods for increasing the specific productivity of an antibody produced in cell culture, increasing cell growth and increasing viability of cells in cell culture. Also provided are, expression stable, viable cell lines comprising DNA encoding adenovirus GAM1 and capable of producing a monoclonal antibody or fragment and/or variant thereof.

    摘要翻译： 本发明涉及产生抗体和其他抗原结合化合物的发酵方法。 具体而言，本发明提供了增加细胞培养中产生的抗体的比生产率，增加细胞生长和增加细胞培养中的细胞活力的方法。 还提供了表达稳定的活细胞系，其包含编码腺病毒GAM1的DNA，并且能够产生单克隆抗体或其片段和/或变体。

    NOVEL METHODS
    13.
    发明申请
    NOVEL METHODS 审中-公开
    新方法

    公开(公告)号：US20100105042A1

    公开(公告)日：2010-04-29

    申请号：US12520244

    申请日：2007-12-20

    申请人： Alexander H. Taylor ,  Delfi Krishna

    发明人： Alexander H. Taylor ,  Delfi Krishna

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1082 ,  C12N15/1086

    摘要： The present invention provides methods for site-specifically integrating at least one first nucleic acid into a genome of at least one cell, comprising: transforming said genome with a reporter nucleic acid construct that is linked to a first att site; selecting at least one first transformed cell having at least one integrated reporter nucleic acid construct; introducing said at least one first nucleic acid and a homologous recombination mediating enzyme into said cell wherein said at least one first nucleic acid comprises at least one second att site that is complementary to said first att site; and maintaining said cell under conditions sufficient for said at least one first nucleic acid to integrate into said first att site producing at least one stably integrated cell.

    摘要翻译： 本发明提供了将至少一种第一核酸位点特异性整合至至少一种细胞的基因组的方法，所述方法包括：用连接到第一att位点的报道核酸构建体转化所述基因组; 选择至少一个具有至少一个整合的报告核酸构建体的第一转化细胞; 将所述至少一种第一核酸和同源重组介导酶引入所述细胞中，其中所述至少一种第一核酸包含与所述第一att位点互补的至少一个第二att位点; 以及在足以使所述至少一种第一核酸整合入所述第一att位点的条件下维持所述细胞，产生至少一个稳定整合的细胞。