公开(公告)号：US20140113300A1

公开(公告)日：2014-04-24

申请号：US14058799

申请日：2013-10-21

Applicant: Raindance Technologies, Inc.

Inventor： Michael Samuels

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2527/107 ,  C12Q2535/131 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: The invention generally relates to methods for increasing the amount of DNA available for analysis when using partitioned samples and parallel processing. For example, double-stranded DNA can be dissociated into two single-stranded components, and the single strands partitioned into different droplets prior to analysis. The disclosed methods are useful for performing digital PCR analysis on samples where the target DNA is not in abundance, for example when the sample originates from a body fluid or an FFPE sample.

Abstract translation: 本发明通常涉及当使用分割样品和并行处理时增加可用于分析的DNA的量的方法。 例如，双链DNA可以解离成两个单链组分，并且在分析之前将单链分成不同的液滴。 所公开的方法对于目标DNA不丰富的样品进行数字PCR分析是有用的，例如当样品来自体液或FFPE样品时。