公开(公告)号：US20090263907A1

公开(公告)日：2009-10-22

申请号：US12377476

申请日：2007-08-27

申请人： Takaharu Asano ,  Katsumi Yabusaki ,  Keiichi Mizushina

发明人： Takaharu Asano ,  Katsumi Yabusaki ,  Keiichi Mizushina

IPC分类号： G01N33/20

CPC分类号： G01N31/22 ,  G01N21/77

摘要： To provide a lead concentration determination reagent which realizes accurate determination of the lead concentration of a sample solution in a simple manner with safety, even when the sample solution contains zinc ions, and a lead concentration determination method making use of the reagent.The lead concentration determination reagent contains (A) a porphyrin derivative and (B) at least one species selected from among ethylenediamine-N,N′-dipropionic acid, ethylenediamine-N,N′-diacetic acid, and 1,6-diaminohexane-N,N,N′,N′-tetraacetic acid.

摘要翻译： 为了提供铅浓度测定试剂，即使在样品溶液含有锌离子的情况下，也能够以简单的方式实现对样品溶液的铅浓度的准确测定，以及利用试剂的铅浓度测定方法。 铅浓度测定试剂含有（A）卟啉衍生物和（B）至少一种选自乙二胺-N，N'-二丙酸，乙二胺-N，N'-二乙酸和1,6-二氨基己烷 - N，N，N'，N'-四乙酸。

公开(公告)号：US06715444B1

公开(公告)日：2004-04-06

申请号：US10129272

申请日：2002-05-03

申请人： Katsumi Yabusaki ,  Hiroshi Nakao ,  Muneharu Ishikawa

发明人： Katsumi Yabusaki ,  Hiroshi Nakao ,  Muneharu Ishikawa

IPC分类号： A01K103

CPC分类号： A01K1/031 ,  A01K29/005 ,  G01H1/12

摘要： A method of measuring the number of times of a specific behavior of an animal in a breeding cage comprising a step in which a common pattern of the specific behavior is determined by receiving a signal of vibration of the cage caused by the specific behavior of the animal and extracting the feature of a frequency component of the signal, a step in which a measurement objective pattern is determined in the same way, and a step in which the common patterns in the measurement objective patterns is counted; a device for the measurement including means for detecting the vibration of the breeding cage, means for extracting the feature of the frequency component, and means for counting the common patterns in the measurement objective patterns; and a method for measuring the specific behavior such as a scratching of a small animal, e.g., a mouse in a breeding cage noninvasively, and an automatic measuring instrument.

摘要翻译： 一种测量育种笼中动物的特定行为次数的方法，包括通过接收由动物的特定行为引起的笼的振动信号来确定特定行为的共同模式的步骤 并提取信号的频率分量的特征，以相同的方式确定测量对象图案的步骤，以及对测量对象图案中的共同图案进行计数的步骤; 用于测量的装置包括用于检测育种笼的振动的装置，用于提取频率分量的特征的装置，以及用于对测量目标图案中的共同图案进行计数的装置; 以及用于非侵入性地测量育种笼中的小动物例如小鼠的特定行为的方法和自动测量仪器。

公开(公告)号：US06667177B1

公开(公告)日：2003-12-23

申请号：US09554056

申请日：2000-05-08

申请人： Katsumi Yabusaki

发明人： Katsumi Yabusaki

IPC分类号： G01N3100

CPC分类号： G01N15/1475 ,  G01N1/30 ,  G01N2015/008 ,  G01N2015/1486 ,  Y10T436/101666 ,  Y10T436/107497

摘要： A leukocyte accumulation container having an opening, a sidewall portion and a bottom portion, part or all of the sidewall portion having a horizontal section area gradually increasing in a direction from the bottom portion towards the opening. A cytolytic agent is added to a solution of a platelet preparation or a solution of an erythrocyte preparation to solubilize platelets or erythrocytes in the solution of the preparation. The leukocyte accumulation container then is set on a centrifuge to accumulate the leukocytes on the bottom portion of the leukocyte accumulation container, and the leukocytes accumulated on the bottom portion are counted.

摘要翻译： 具有开口，侧壁部分和底部的白细胞聚集容器，所述侧壁部分的一部分或全部具有在从底部朝向开口的方向上逐渐增加的水平截面积。 将细胞溶解剂加入到血小板制剂或红细胞制剂溶液中以在制剂溶液中溶解血小板或红细胞。 然后将白细胞积聚容器置于离心机上以将白细胞积聚在白细胞积聚容器的底部，并计数累积在底部的白细胞。

公开(公告)号：US20120003745A1

公开(公告)日：2012-01-05

申请号：US13256427

申请日：2010-03-12

申请人： Katsumi Yabusaki ,  Takuya Hara ,  Yuka Sugiura

发明人： Katsumi Yabusaki ,  Takuya Hara ,  Yuka Sugiura

IPC分类号： G01N33/00 ,  G01N27/00

CPC分类号： G01N33/579 ,  G01N21/82 ,  Y10T436/143333

摘要： When assessing the start time of the limulus reaction between biogenous biologically active substances and LAL and using the reaction start time to determine the concentration of the biogenous biologically active substances, in order to exclude the influence of progressive changes which occur regardless of the conditions of the limulus reaction, the strength of transmitted light or scattered light in the liquid mixture of the measurement sample and LAL is detected, the variation (delta) in the transmittance or number of gel particles is acquired at set intervals, and the time when the variation (delta) crosses a threshold value is taken as the reaction start time. Furthermore, the time intervals when acquiring the abovementioned delta are not uniform, and either change over time from the start of measurement as defined by a time function, or multiple sequences with differing time intervals are prepared in advance.

摘要翻译： 当评估生物生物活性物质与LAL之间的鲎反应的开始时间并且使用反应开始时间来确定生物生物活性物质的浓度时，为了排除发生的进行性变化的影响，而不管 鲎反应检测到测量样品和LAL的液体混合物中的透射光或散射光的强度，凝胶颗粒的透射率或数量的变化（delta）以设定的间隔获得，并且变化（ delta）跨越阈值作为反应开始时间。 此外，获取上述增量的时间间隔是不均匀的，并且随着时间函数定义的测量开始的时间而改变，或者预先准备具有不同时间间隔的多个序列。

公开(公告)号：US20110201015A1

公开(公告)日：2011-08-18

申请号：US12809337

申请日：2008-12-18

申请人： Katsumi Yabusaki

发明人： Katsumi Yabusaki

IPC分类号： G01N33/579

CPC分类号： C12Q1/37 ,  G01N33/54313 ,  G01N33/579

摘要： A technique which is less likely to be affected by turbid or color of samples and with which prompt and simple detection or concentration measurement of endotoxin can be achieved is provided. A reagent in which proteins contained in LAL are adsorbed or bound onto fine particles dispersed in a previously prepared drug liquid is prepared, and this reagent and a sample containing endotoxin are mixed. By doing this, endotoxin acts on the proteins on the fine particles and the fine particles are associated with one another to form a large aggregate at an early stage. By optically measuring the formation of this aggregate, detection or concentration measurement of endotoxin is performed.

摘要翻译： 提供了不太可能受到样品的混浊或颜色影响的技术，并且可以实现对内毒素的即时和简单的检测或浓度测量。 准备将LAL中含有的蛋白质吸附或结合到分散在预先制备的药液中的微粒子上的试剂，并将该试剂和含有内毒素的试样混合。 通过这样做，内毒素作用于细颗粒上的蛋白质，并且细颗粒彼此相关联以在早期形成大的聚集体。 通过光学测量该聚集体的形成，进行内毒素的检测或浓度测定。

公开(公告)号：US20110124036A1

公开(公告)日：2011-05-26

申请号：US13056100

申请日：2009-07-28

申请人： Katsumi Yabusaki

发明人： Katsumi Yabusaki

IPC分类号： C12Q1/02 ,  C12M1/34

CPC分类号： G01N21/51 ,  G01N21/77 ,  G01N21/82 ,  G01N33/579 ,  G01N2021/825

摘要： Disclosed is a measurement method which can largely reduce the time required for the detection of a physiologically active substance derived from an organism (e.g., an endotoxin, β-D-glucan) or the determination of the concentration of the physiologically active substance by utilizing the reaction between the physiologically active substance and LAL. Also disclosed is a measurement apparatus utilizing the measurement method. An incident light from a light source is focused onto a sample and delivered to the sample to cause the bombardment with coagulin which is a final product of a protease cascade (i.e., a coagulin monomer) and an extremely fine aggregate which is produced by the aggregation of the coagulin monomers (i.e. a coagulin aggregate), thereby generating a scattered light. The scattered light is detected by a light-receiving element. The concentration of the endotoxin can be determined based on the initial rate of increase in the scattered light detected.

摘要翻译： 公开了一种测量方法，其可以大大减少检测来自生物体的生理活性物质（例如内毒素，β-葡聚糖）或通过利用生物活性物质的浓度的测定所需的时间 生理活性物质与LAL之间的反应。 还公开了利用该测量方法的测量装置。 将来自光源的入射光聚焦在样品上并传送到样品中，以凝结蛋白（其为蛋白酶级联的最终产物（即凝固素单体））和由聚集产生的极细骨料进行轰击 的凝血酶单体（即凝血酶聚集体），从而产生散射光。 散射光由光接收元件检测。 可以基于检测到的散射光的初始增加速率来确定内毒素的浓度。

公开(公告)号：US20110091902A1

公开(公告)日：2011-04-21

申请号：US12996312

申请日：2009-06-04

申请人： Katsumi Yabusaki

发明人： Katsumi Yabusaki

IPC分类号： G01N33/53 ,  C12Q1/37 ,  C12M1/34

CPC分类号： G01N21/82 ,  G01N33/579

摘要： Provided is a technique whereby a physiologically active substance of biological origin such as an endotoxin or β-D-glucan can be more conveniently and accurately detected and the concentration thereof can be determined even in the case of using a sample which contains a substance affecting LAL activity. For example, a sample is brought into contact with a substance (2) capable of adsorbing an endotoxin and thus the endotoxin contained in the sample is preliminarily adsorbed. Next, the sample per se is washed away and the adsorbed endotoxin (4) is reacted with LAL. Then the gelation of LAL or the formation of gel particles (5) is detected to thereby detecting the endotoxin and determining the concentration thereof.

摘要翻译： 本发明提供一种能够更方便，准确地检测生物来源的生物活性物质，例如内毒素或β-葡聚糖的技术，即使在使用含有影响物质的样品的情况下也可以测定其浓度 LAL活动。 例如，使样品与能够吸附内毒素的物质（2）接触，从而预先吸附样品中所含的内毒素。 接下来，洗涤样品本身并吸附的内毒素（4）与LAL反应。 然后检测LAL的凝胶化或凝胶颗粒（5）的形成，从而检测内毒素并测定其浓度。

公开(公告)号：US07846339B2

公开(公告)日：2010-12-07

申请号：US11993588

申请日：2006-06-26

申请人： Hironori Suzuki ,  Katsumi Yabusaki

发明人： Hironori Suzuki ,  Katsumi Yabusaki

IPC分类号： C02F1/58 ,  C02F101/10

CPC分类号： B03D3/06 ,  B03D3/02 ,  C02F1/56 ,  C02F1/66 ,  C02F2101/10 ,  C02F2103/12 ,  C02F2103/16 ,  Y10S210/902

摘要： Boron is removed from an aqueous boron solution by contacting with the aqueous boron solution with a water-soluble cationic polyelectrolyte, and at least one of polyvinyl alcohol and anionic-modified polyvinyl alcohol under an alkaline condition, and then removing the generated coagulation.

摘要翻译： 通过与硼酸水溶液与水溶性阳离子聚电解质和在碱性条件下与聚乙烯醇和阴离子改性聚乙烯醇中的至少一种接触，然后除去所产生的凝结物，从硼水溶液中除去硼。

公开(公告)号：US20070217974A1

公开(公告)日：2007-09-20

申请号：US11575098

申请日：2005-09-26

申请人： Ming Lu ,  Katsumi Yabusaki

发明人： Ming Lu ,  Katsumi Yabusaki

IPC分类号： C08B13/00 ,  B01J20/26

CPC分类号： C08B5/00 ,  B01J20/26 ,  B01J20/262 ,  B01J20/265 ,  C08B11/04 ,  C08B11/08 ,  C08B11/20 ,  C08B13/00

摘要： The present invention is directed to a phosphoric ester of 2,3-dihydroxypropyl cellulose or 3-(2,3-dihydroxypropoxy)-2-hydroxypropyl cellulose, the cellulose having optionally undergone partial carbamidation, and to a metal absorbent employing the ester, and to a metal-absorbing apparatus employing the metal adsorbent. The cellulose derivative phosphoric ester serves as a metal adsorbent which exhibits high metal adsorption performance and metal adsorption rate per unit weight; has high mechanical strength; effectively soften water or a similar liquid; removes heavy metals from wastewater or a similar liquid at remarkably high efficiency; has excellent processability and can be formed into a variety of shapes and thus is applicable within a wide range: and can readily be reutilized.

摘要翻译： 本发明涉及2,3-二羟基丙基纤维素或3-（2,3-二羟基丙氧基）-2-羟丙基纤维素的磷酸酯，该纤维素任选经历部分氨基甲酰化，以及使用该酯的金属吸收剂，以及 涉及使用金属吸附剂的金属吸收装置。 纤维素衍生物磷酸酯用作金属吸附剂，其具有高的金属吸附性能和每单位重量的金属吸附速率; 机械强度高; 有效地软化水或类似液体; 以非常高的效率从废水或类似液体中除去重金属; 具有优异的加工性，可以形成各种形状，因此可以在宽范围内使用，并且可以容易地再利用。

公开(公告)号：US08507282B2

公开(公告)日：2013-08-13

申请号：US13256427

申请日：2010-03-12

申请人： Katsumi Yabusaki ,  Takuya Hara ,  Yuka Sugiura

发明人： Katsumi Yabusaki ,  Takuya Hara ,  Yuka Sugiura

IPC分类号： G01N33/92 ,  G01N33/48 ,  G01N21/47 ,  G01N21/64 ,  G01N21/76 ,  G01N21/82

CPC分类号： G01N33/579 ,  G01N21/82 ,  Y10T436/143333

摘要： When assessing the start time of the limulus reaction between biogenous biologically active substances and LAL and using the reaction start time to determine the concentration of the biogenous biologically active substances, in order to exclude the influence of progressive changes which occur regardless of the conditions of the limulus reaction, the strength of transmitted light or scattered light in the liquid mixture of the measurement sample and LAL is detected, the variation (delta) in the transmittance or number of gel particles is acquired at set intervals, and the time when the variation (delta) crosses a threshold value is taken as the reaction start time. Furthermore, the time intervals when acquiring the abovementioned delta are not uniform, and either change over time from the start of measurement as defined by a time function, or multiple sequences with differing time intervals are prepared in advance.

摘要翻译： 当评估生物生物活性物质与LAL之间的鲎反应的开始时间并且使用反应开始时间来确定生物生物活性物质的浓度时，为了排除发生的进行性变化的影响，而不管 鲎反应检测到测量样品和LAL的液体混合物中的透射光或散射光的强度，凝胶颗粒的透射率或数量的变化（delta）以设定的间隔获得，并且变化（ delta）跨越阈值作为反应开始时间。 此外，获取上述增量的时间间隔是不均匀的，并且随着时间函数定义的测量开始的时间而改变，或者预先准备具有不同时间间隔的多个序列。