公开(公告)号：US5607842A

公开(公告)日：1997-03-04

申请号：US230427

申请日：1994-04-19

申请人： Stanley N. Cohen ,  Martin Vogtli

发明人： Stanley N. Cohen ,  Martin Vogtli

IPC分类号： C12N15/63 ,  C12N15/76 ,  C12P21/02 ,  C12N1/21 ,  C12N15/70 ,  C12N15/74

CPC分类号： C12N15/76 ,  C12N15/63

摘要： Expression systems are provided, where plasmids can be safely maintained in a prokaryotic host, by providing for a tRNA gene for an essential tRNA in a tRNA gene negative background. A non-selective medium can be employed to permit vigorous growth of the host and efficient expression of a protein of interest.

摘要翻译： 提供表达系统，其中通过在tRNA基因阴性背景中提供tRNA基因作为必需tRNA，可以将质粒安全地维持在原核宿主中。 可以使用非选择性培养基来允许宿主的剧烈生长和目的蛋白质的有效表达。

公开(公告)号：US4468464A

公开(公告)日：1984-08-28

申请号：US959288

申请日：1978-11-09

申请人： Stanley N. Cohen ,  Herbert W. Boyer

发明人： Stanley N. Cohen ,  Herbert W. Boyer

IPC分类号： C12N15/00 ,  C12N1/00 ,  C12N1/20 ,  C12P21/00

CPC分类号： C12N15/00 ,  Y10S435/82

摘要： Method and compositions are provided for replication and expression of exogenous genes in microorganisms. Plasmids or virus DNA are cleaved to provide linear DNA having ligatable termini, which are bound to a gene having complementary termini, to provide a biologically functional replicon with a desired phenotypical property. The replicon is inserted into a microorganism cell by transformation. Isolation of the transformants provides cells for replication and expression of the DNA molecules present in the modified plasmid. The method provides a convenient and efficient way to introduce genetic capability into microorganisms for the production of nucleic acids are proteins, such as medically or commercially useful enzymes, which may have direct usefulness, or may find expression in the production of drugs, such as hormones, antibiotics, or the like, fixation of nitrogen, fermentation, utilization of specific feedstocks, or the like.The invention was supported by generous grants of NIH, NSF and the American Cancer Society.

摘要翻译： 提供了方法和组合物用于在微生物中复制和表达外源基因。 切割质粒或病毒DNA以提供具有可连接末端的线性DNA，其与具有互补末端的基因结合，以提供具有期望表型性质的生物功能复制子。 通过转化将复制子插入微生物细胞。 转化体的分离提供细胞用于复制和表达修饰质粒中存在的DNA分子。 该方法提供了将遗传能力引入微生物以便生产核酸的方便和有效的方法是蛋白质，例如可能具有直接有用性的蛋白质，例如医学或商业上有用的酶，或可能在药物生产中发现，例如激素 ，抗生素等，固氮，发酵，利用特定原料等。 该发明得到NIH，NSF和美国癌症协会的大量赠款的支持。

公开(公告)号：US20110251376A1

公开(公告)日：2011-10-13

申请号：US13116911

申请日：2011-05-26

申请人： Stanley N. Cohen ,  Limin Li

发明人： Stanley N. Cohen ,  Limin Li

IPC分类号： C07K19/00 ,  C07K16/18

CPC分类号： G01N33/57415 ,  C07K14/4702 ,  C12N15/00 ,  C12Q1/68 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/136

摘要： TSG101 is a tumor susceptibility gene whose homozygous functional knock out in fibroblasts leads to transformation and the ability of these cells to form metastatic tumors in nude mice. The cellular transformation that results from inactivation of TSG101 is reversible by restoration of TSG101 function. Decreased expression of TSG101 is associated with the occurrence of certain human cancers, including breast carcinomas. The TSG101 nucleic acid compositions find use in identifying homologous or related proteins and the DNA sequences encoding such proteins; in producing compositions that modulate the expression or function of the protein; and in studying associated physiological pathways. In addition, modulation of the gene activity in vivo is used for prophylactic and therapeutic purposes, such as treatment of cancer, identification of cell type based on expression, and the like. The DNA is further used as a diagnostic for a genetic predisposition to cancer, and to identify specific cancers having mutations in this gene.

摘要翻译： TSG101是一种肿瘤易感基因，其纯合功能敲除成纤维细胞导致转化和这些细胞在裸鼠中形成转移性肿瘤的能力。 由TSG101失活引起的细胞转化可通过恢复TSG101功能而逆转。 TSG101的表达降低与某些人类癌症（包括乳腺癌）的发生有关。 TSG101核酸组合物用于鉴定同源或相关蛋白质和编码此类蛋白质的DNA序列; 在制备调节蛋白质的表达或功能的组合物中; 并研究相关的生理学途径。 此外，体内基因活性的调节用于预防和治疗目的，例如癌症的治疗，基于表达的细胞类型的鉴别等。 该DNA进一步用于诊断癌症的遗传易感性，并鉴定具有该基因突变的特异性癌症。

公开(公告)号：US20100272728A1

公开(公告)日：2010-10-28

申请号：US12721583

申请日：2010-03-11

申请人： Limin Li ,  Stanley N. Cohen

发明人： Limin Li ,  Stanley N. Cohen

IPC分类号： A61K39/395 ,  A61P35/00

CPC分类号： C07K14/47 ,  C07K16/18

摘要： The present invention provides methods and compositions for regulating ubiquitination in a cell. In particular, the present invention provides purified polypeptides comprising an ubiquitination-regulating domain. The invention also provides methods of using such polypeptides for screening for agents, for producing antibodies, and for treatment of diseases, e.g., proliferative diseases, neurodegenerative diseases, autoimmune diseases, metabolic disease and developmental abnormalities. The invention further provides antibodies that bind an ubiquitination-regulating domain and agents and antibodies that regulate ubiquitination in cells, e.g., by modulating the interaction between a TSG101 protein and an MDM2 protein.

摘要翻译： 本发明提供用于调节细胞中泛素化的方法和组合物。 特别地，本发明提供了包含泛素调节结构域的纯化多肽。 本发明还提供了使用这种多肽筛选试剂，产生抗体和治疗疾病如增殖性疾病，神经变性疾病，自身免疫性疾病，代谢疾病和发育异常的方法。 本发明还提供结合泛素调节结构域的抗体以及调节细胞中的泛素化的试剂和抗体，例如通过调节TSG101蛋白和MDM2蛋白之间的相互作用。

公开(公告)号：US06835816B2

公开(公告)日：2004-12-28

申请号：US09804690

申请日：2001-03-12

申请人： Stanley N. Cohen ,  Limin Li

发明人： Stanley N. Cohen ,  Limin Li

IPC分类号： C07K1600

CPC分类号： G01N33/57415 ,  C07K14/4702 ,  C12N15/00 ,  C12Q1/68 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/136

摘要： TSG101 is a tumor susceptibility gene whose homozygous functional knock out in fibroblasts leads to transformation and the ability of these cells to form metastatic tumors in nude mice. The cellular transformation that results from inactivation of TSG101 is reversible by restoration of TSG101 function. Decreased expression of TSG101 is associated with the occurrence of certain human cancers, including breast carcinomas. The TSG101 nucleic acid compositions find use in identifying homologous or related proteins and the DNA sequences encoding such proteins; in producing compositions that modulate the expression or function of the protein; and in studying associated physiological pathways. In addition, modulation of the gene activity in vivo is used for prophylactic and therapeutic purposes, such as treatment of cancer, identification of cell type based on expression, and the like. The DNA is further used as a diagnostic for a genetic predisposition to cancer, and to identify specific cancers having mutations in this gene.

摘要翻译： TSG101是一种肿瘤易感基因，其纯合功能敲除成纤维细胞导致转化和这些细胞在裸鼠中形成转移性肿瘤的能力。 由TSG101失活引起的细胞转化可通过恢复TSG101功能而逆转。 TSG101的表达降低与某些人类癌症（包括乳腺癌）的发生有关。 TSG101核酸组合物用于鉴定同源或相关蛋白质和编码此类蛋白质的DNA序列; 在制备调节蛋白质的表达或功能的组合物中; 并研究相关的生理学途径。 此外，体内基因活性的调节用于预防和治疗目的，例如癌症的治疗，基于表达的细胞类型的鉴别等。 该DNA进一步用于诊断癌症的遗传易感性，并鉴定具有该基因突变的特异性癌症。

公开(公告)号：US06248523B1

公开(公告)日：2001-06-19

申请号：US09146187

申请日：1998-09-01

申请人： Stanley N. Cohen ,  Limin Li

发明人： Stanley N. Cohen ,  Limin Li

IPC分类号： C12Q168

CPC分类号： G01N33/57415 ,  C07K14/4702 ,  C12N15/00 ,  C12Q1/68 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/136

摘要： TSG101 is a tumor susceptibility gene whose homozygous functional knock out in fibroblasts leads to transformation and the ability of these cells to form metastatic tumors in nude mice. The cellular transformation that results from inactivation of TSG101 is reversible by restoration of TSG101 function. Decreased expression of TSG101 is associated with the occurrence of certain human cancers, including breast carcinomas. The TSG101 nucleic acid compositions find use in identifying homologous or related proteins and the DNA sequences encoding such proteins; in producing compositions that modulate the expression or function of the protein; and in studying associated physiological pathways. In addition, modulation of the gene activity in vivo is used for prophylactic and therapeutic purposes, such as treatment of cancer, identification of cell type based on expression, and the like. The DNA is further used as a diagnostic for a genetic predisposition to cancer, and to identify specific cancers having mutations in this gene.

摘要翻译： TSG101是一种肿瘤易感基因，其纯合功能敲除成纤维细胞导致转化和这些细胞在裸鼠中形成转移性肿瘤的能力。 由TSG101失活引起的细胞转化可通过恢复TSG101功能而逆转。 TSG101的表达降低与某些人类癌症（包括乳腺癌）的发生有关。 TSG101核酸组合物用于鉴定同源或相关蛋白质和编码此类蛋白质的DNA序列; 在制备调节蛋白质的表达或功能的组合物中; 并研究相关的生理学途径。 此外，体内基因活性的调节用于预防和治疗目的，例如癌症的治疗，基于表达的细胞类型的鉴别等。 该DNA进一步用于诊断癌症的遗传易感性，并鉴定具有该基因突变的特异性癌症。

公开(公告)号：US5891668A

公开(公告)日：1999-04-06

申请号：US670274

申请日：1996-06-13

申请人： Limin Li ,  Stanley N. Cohen

发明人： Limin Li ,  Stanley N. Cohen

IPC分类号： C07K14/47 ,  C12N15/00 ,  C12Q1/68 ,  C12P21/02 ,  C12N15/12

CPC分类号： C12N15/00 ,  C07K14/4702 ,  C12Q1/68

摘要： TSG101 is a tumor susceptibility gene whose homozygous functional knock out in fibroblasts leads to transformation and the ability of these cells to form metastatic tumors in nude mice. The cellular transformation that results from inactivation of TSG101 is reversible by restoration of TSG101 function. Decreased expression of TSG101 is associated with the occurrence of certain human cancers, including breast carcinomas. The TSG101 nucleic acid compositions find use in identifying homologous or related proteins and the DNA sequences encoding such proteins; in producing compositions that modulate the expression or function of the protein; and in studying associated physiological pathways. In addition, modulation of the gene activity in vivo is used for prophylactic and therapeutic purposes, such as treatment of cancer, identification of cell type based on expression, and the like. The DNA is further used as a diagnostic for a genetic predisposition to cancer, and to identify specific cancers having mutations in this gene.

公开(公告)号：US20100272729A1

公开(公告)日：2010-10-28

申请号：US12721584

申请日：2010-03-11

申请人： LIMIN LI ,  Stanley N. Cohen

发明人： LIMIN LI ,  Stanley N. Cohen

IPC分类号： A61K39/395 ,  C07K16/18 ,  A61P35/00

CPC分类号： C07K14/47 ,  C07K16/18

摘要： The present invention provides methods and compositions for regulating ubiquitination in a cell. In particular, the present invention provides purified polypeptides comprising an ubiquitination-regulating domain. The invention also provides methods of using such polypeptides for screening for agents, for producing antibodies, and for treatment of diseases, e.g., proliferative diseases, neurodegenerative diseases, autoimmune diseases, metabolic disease and developmental abnormalities. The invention further provides antibodies that bind an ubiquitination-regulating domain and agents and antibodies that regulate ubiquitination in cells, e.g., by modulating the interaction between a TSG101 protein and an MDM2 protein.

公开(公告)号：US20090233993A1

公开(公告)日：2009-09-17

申请号：US12399850

申请日：2009-03-06

申请人： Huaxi Xu ,  Limin Li ,  Francesca-Fang Liao ,  Yun-wu Zhang ,  Wu-bo Li ,  Paul Greengard ,  Stanley N. Cohen

发明人： Huaxi Xu ,  Limin Li ,  Francesca-Fang Liao ,  Yun-wu Zhang ,  Wu-bo Li ,  Paul Greengard ,  Stanley N. Cohen

IPC分类号： A61K31/7088 ,  C07H21/00 ,  C12N15/74 ,  C12P21/02 ,  C07K2/00 ,  C12N5/06 ,  A61P25/28 ,  G01N33/567

CPC分类号： C07K14/705 ,  A61K38/00 ,  G01N2333/91205 ,  G01N2800/2821

摘要： Disclosed herein are compositions and methods relating to peptides. The peptides can inhibit amyloid beta (Aβ) generation and reduce GSK-3 activities. Further provided are compositions and methods for treating or preventing, for example, Alzheimer's disease, cancer, and diabetes.

摘要翻译： 本文公开了与肽相关的组合物和方法。 肽可以抑制淀粉样蛋白β（Abeta）的产生并降低GSK-3活性。 还提供了用于治疗或预防例如阿尔茨海默病，癌症和糖尿病的组合物和方法。

公开(公告)号：US07494767B2

公开(公告)日：2009-02-24

申请号：US10478753

申请日：2002-05-21

申请人： Carol Carter ,  Arthur Goff ,  Lorna Ehrlich ,  Stanley N. Cohen

发明人： Carol Carter ,  Arthur Goff ,  Lorna Ehrlich ,  Stanley N. Cohen

IPC分类号： C12Q1/00 ,  C12Q1/70 ,  C12Q1/68 ,  G01N33/53

CPC分类号： G01N33/502 ,  A61K38/1709 ,  C07K14/005 ,  C07K14/4702 ,  C07K2319/00 ,  C12N2740/16222 ,  G01N33/5008 ,  G01N33/56983 ,  G01N33/56988 ,  G01N33/6878 ,  G01N2333/16 ,  G01N2333/161 ,  G01N2500/02 ,  G01N2500/10 ,  G01N2500/20

摘要： The present invention provides methods for identifying peptides in a mammalian. Tsg101 protein that binds to the PTAPP (SEQ ID NO: 3) motif or L domain of human immunodeficiency virus type I (HIV-1). Such peptides can be used to inhibit Tsg101-HIV Gag binding, and is therefore effective in reducing HIV particle production. The invention also provides the peptides identified by the method of the invention and to method of using such peptides for treating HIV infection.

摘要翻译： 本发明提供用于鉴定哺乳动物中的肽的方法。 结合人类免疫缺陷病毒I型（HIV-1）的PTAPP（SEQ ID NO：3）基序或L结构域的Tsg101蛋白。 这样的肽可用于抑制Tsg101-HIV Gag结合，因此有效地减少HIV颗粒的产生。 本发明还提供了通过本发明的方法鉴定的肽以及使用这些肽来治疗HIV感染的方法。