公开(公告)号：US20220073999A1

公开(公告)日：2022-03-10

申请号：US17514724

申请日：2021-10-29

Applicant: The University of Kansas ,  Kansas State University Research Foundation

Inventor： Mei He

IPC: C12Q1/6886 ,  A61K47/69 ,  G01N33/574 ,  G01N33/543 ,  B03C1/015 ,  B03C1/30 ,  B01L3/00 ,  A61K49/00

Abstract: Methods for producing engineered exosomes and other vesicle-like biological targets, including allowing a target vesicle-like structure to react and bind with immunomagnetic particles; capturing the immunomagnetic particle/vesicle complex by applying a magnetic field; further engineering the captured vesicles by surface modifying with additional active moieties or internally loading with active agents; and releasing the engineered vesicle-like structures, such as by photolytically cleaving a linkage between the particle and engineered vesicle-like structures, thereby releasing intact vesicle-like structures which can act as delivery vehicles for therapeutic treatments.

公开(公告)号：US11162143B2

公开(公告)日：2021-11-02

申请号：US17236561

申请日：2021-04-21

Applicant: The University of Kansas ,  Kansas State University Research Foundation

Inventor： Mei He

IPC: G01N27/00 ,  B03C5/00 ,  G01N27/12 ,  C12Q1/6886 ,  A61K47/69 ,  G01N33/574 ,  G01N33/543 ,  B03C1/015 ,  B03C1/30 ,  B01L3/00 ,  A61K49/00

Abstract: Methods for producing engineered exosomes and other vesicle-like biological targets, including allowing a target vesicle-like structure to react and bind with immunomagnetic particles; capturing the immunomagnetic particle/vesicle complex by applying a magnetic field; further engineering the captured vesicles by surface modifying with additional active moieties or internally loading with active agents; and releasing the engineered vesicle-like structures, such as by photolytically cleaving a linkage between the particle and engineered vesicle-like structures, thereby releasing intact vesicle-like structures which can act as delivery vehicles for therapeutic treatments.

公开(公告)号：US10350599B2

公开(公告)日：2019-07-16

申请号：US15357231

申请日：2016-11-21

Applicant: University of Kansas ,  Kansas State University Research Foundation

Inventor： Zheng Zhao ,  Mei He ,  Yong Zeng

IPC: B01F5/06 ,  G01N33/574 ,  B01L3/00 ,  B01F13/00

Abstract: A microfluidic exosome profiling platform integrating exosome isolation and targeted proteomic analysis is disclosed. This platform is capable of quantitative exosomal biomarker profiling directly from plasma samples with markedly enhanced sensitivity and specificity. Identification of distinct subpopulation of patient-derived exosomes is demonstrated by probing surface proteins and multiparameter analyzes of intravesicular biomarkers in the selected subpopulation. The expression of IGF-1R and its phosphorylation level in non-small cell lung cancer (NSCLC) patient plasma is assessed as a non-invasive alternative to the conventional biopsy and immunohistochemistry. Detection of ovarian cancer also is assessed. The microfluidic chip, which may be fabricated of a glass substrate and a layer of poly(dimethylsiloxane), includes a serpentine microchannel to mix a fluid and a microchamber for the collection and detection of exosomes.