公开(公告)号：US20030125496A1

公开(公告)日：2003-07-03

申请号：US10269561

申请日：2002-10-11

Inventor： Todd C. Strother ,  Lloyd M. Smith ,  Robert J. Hamers

IPC: B05D003/00 ,  C12M001/34 ,  C08G002/00 ,  C08G075/04

CPC classification number: B82Y30/00 ,  B05D1/185 ,  B82Y10/00 ,  B82Y40/00 ,  H01L51/0093

Abstract: Chemically-modified surfaces on unoxidized carbon, silicon, and germanium substrates are disclosed. Ultraviolet radiation mediates the reaction of protected null-modified, null-unsaturated aminoalkenes (preferred) with hydrogen-terminated carbon, silicon, or germanium surfaces. Removal of the protecting group yields an aminoalkane-modified silicon surface These amino groups can be coupled to terminal-modified oligonucleotides using a bifunctional crosslinker, thereby permitting the preparation of modified surfaces and arrays. Methods for controlling the surface density of molecules attached to the substrate are also disclosed.

Abstract translation: 公开了未氧化的碳，硅和锗衬底上的化学修饰的表面。 紫外线辐射介导受保护的ω-改性的α-不饱和氨基烯烃（优选）与氢封端的碳，硅或锗表面的反应。 保护基团的去除产生氨基烷烃改性的硅表面这些氨基可以使用双官能交联剂与末端修饰的寡核苷酸偶联，从而允许制备改性的表面和阵列。 还公开了用于控制附着于基底的分子的表面密度的方法。