公开(公告)号：US06599696B2

公开(公告)日：2003-07-29

申请号：US10139586

申请日：2002-05-02

Applicant: Merle Edwin Olson ,  Howard Ceri

Inventor： Merle Edwin Olson ,  Howard Ceri

IPC: C12Q100

CPC classification number: C12M25/00 ,  C12M23/12 ,  C12M25/06

Abstract: A method for testing the performance of surface coatings on the formation of biofilm and encrustation on materials. The method includes, providing a plurality of material retaining sites, providing the material retaining sites with a material, wherein the material models a surface likely to be involved in biofilm formation and encrustation deposit. The method further includes the steps of providing a liquid growth medium, wherein the liquid growth medium includes at least one microorganism, the liquid growth medium arranged to cover at least a portion of the material, and incubating microorganism and encrustation on the material retaining sites in the presence of the liquid growth medium.

Abstract translation: 一种用于测试表面涂层对材料形成生物膜和结垢的性能的方法。 该方法包括提供多个材料保留位置，为材料保留位置提供材料，其中该材料模拟可能参与生物膜形成和结垢沉积的表面。 该方法还包括提供液体生长培养基的步骤，其中液体生长培养基包括至少一种微生物，液体生长培养基布置成覆盖材料的至少一部分，并将微生物和结垢保温在材料保留位置上 存在液体生长培养基。

公开(公告)号：US06410256B1

公开(公告)日：2002-06-25

申请号：US09380714

申请日：2000-07-19

Applicant: Howard Ceri ,  Merle Edwin Olson ,  Douglas Walter Morck ,  Ronald Rae Read ,  Andre Gerald Buret

Inventor： Howard Ceri ,  Merle Edwin Olson ,  Douglas Walter Morck ,  Ronald Rae Read ,  Andre Gerald Buret

IPC: C12Q102

CPC classification number: B01L3/50853 ,  C12M23/38 ,  C12M25/06 ,  C12M27/16 ,  C12M35/04 ,  C12N9/20

Abstract: Biofilm forming organisms are incubated to form a biofilm on projections by providing a flow of liquid growth medium across projections, the direction of the flow of liquid being repeatedly changed, and an assay made of the resulting biofilm. Biofilm forming organisms are incubated to form a biofilm on projections arranged in rows, with several projections in each row, while providing a flow of liquid growth medium across each row of projections, and an assay made of the resulting biofilm. Sensitivity of the biofilm to antimicrobial reagent may be determined by treating the projections with antimicrobial reagent before carrying out the assay, by treating each row of projections with a different antimicrobial reagent, and each of the projections in a row with a different concentration of antimicrobial reagent.

Abstract translation: 将生物膜形成生物体孵育以在突起上形成生物膜，通过提供液体生长培养基跨过突起的流动，液体的流动方向被重复改变，以及由所得生物膜制成的测定。 将生物膜形成生物体孵育以在排列成排的突起上形成生物膜，每行中具有若干突起，同时在每排突起处提供液体生长介质流，以及由所得生物膜制成的测定。 生物膜对抗微生物试剂的敏感性可以通过在进行测定之前用抗微生物剂处理突出物，通过用不同的抗微生物试剂处理每一排突起，并且将每个突起以不同浓度的抗微生物剂 。