公开(公告)号：US20060234379A1

公开(公告)日：2006-10-19

申请号：US11405106

申请日：2006-04-17

申请人： Hee-kyun Lim ,  Kyu-youn Hwang ,  Joon-ho Kim ,  Kak Namkoong ,  Chin-sung Park

发明人： Hee-kyun Lim ,  Kyu-youn Hwang ,  Joon-ho Kim ,  Kak Namkoong ,  Chin-sung Park

IPC分类号： C12N5/02 ,  C12N5/00

CPC分类号： B01L3/502753 ,  B01L3/502746 ,  B01L2300/0681 ,  B01L2400/086 ,  B82Y30/00 ,  B82Y40/00 ,  C12M47/02

摘要： Provided is a method of separating cells using a hydrophobic solid support. The method comprises contacting a solution containing cells with a hydrophobic solid support having a water contact angle between 70 and 90 degrees. By allowing cells to be adsorbed to the hydrophobic solid substrate, the cells can be separated. Thus, the cell separation efficiency can be rapidly and simply increased.

摘要翻译： 提供了使用疏水性固体支持物分离细胞的方法。 该方法包括将含有细胞的溶液与70-90度的水接触角的疏水性固体支持物接触。 通过使细胞被吸附到疏水性固体底物上，可以分离细胞。 因此，可以快速且简单地增加细胞分离效率。

公开(公告)号：US20060166241A1

公开(公告)日：2006-07-27

申请号：US11325834

申请日：2006-01-05

申请人： Jeo-young Shim ,  Kyu-youn Hwang ,  Joon-ho Kim ,  Sung-ouk Jung ,  Kyu-tae Yoo ,  Joon-shik Park ,  Ji-na Namgoong

发明人： Jeo-young Shim ,  Kyu-youn Hwang ,  Joon-ho Kim ,  Sung-ouk Jung ,  Kyu-tae Yoo ,  Joon-shik Park ,  Ji-na Namgoong

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12N15/1006 ,  C12Q1/6806 ,  C12Q2563/173 ,  C12Q2523/308

摘要： Provided are nucleic acid isolation unit and method. The method includes immobilizing an aromatic compound-containing nucleic acid intercalator on a solid support; contacting a first buffer solution containing a nucleic acid sample to be purified to the intercalator immobilized on the solid support to bind the intercalator with nucleic acids contained in the nucleic acid sample; cleaning the resultant structure where the nucleic acids are bound to the intercalator immobilized on the solid support; and eluting the nucleic acids with a second buffer solution.

摘要翻译： 提供了核酸分离单元和方法。 该方法包括将含芳族化合物的核酸嵌入剂固定在固体支持物上; 将含有要纯化的核酸样品的第一缓冲溶液与固定在固体支持物上的嵌入剂接触，以使嵌入剂与核酸样品中所含的核酸结合; 清洗所得结构，其中核酸与固定在固相载体上的嵌入剂结合; 并用第二缓冲溶液洗脱核酸。

公开(公告)号：US20060154284A1

公开(公告)日：2006-07-13

申请号：US11317949

申请日：2005-12-23

申请人： Kyu-youn Hwang ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hun-joo Lee ,  Hee-kyun Lim ,  Sung-young Jeong ,  Jeo-young Shim

发明人： Kyu-youn Hwang ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hun-joo Lee ,  Hee-kyun Lim ,  Sung-young Jeong ,  Jeo-young Shim

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/1006 ,  B01D15/361 ,  B01D15/364 ,  B01J20/3285 ,  B01J43/00 ,  C12Q1/6806 ,  C12Q2527/119 ,  C12Q2523/308

摘要： A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.

摘要翻译： 提供了从含有核酸的样品中分离核酸的方法。 该方法包括使样品与含有氨基和羧基的双官能材料接触并在第一pH下带正电荷以允许核酸与双功能材料的结合; 以及从所述络合物中提取比所述第一pH高的第二pH的所述核酸。

公开(公告)号：US20060134777A1

公开(公告)日：2006-06-22

申请号：US11304083

申请日：2005-12-15

申请人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

发明人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

IPC分类号： G01N27/447 ,  C12M1/33

CPC分类号： C12N1/066 ,  C12M47/06

摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

公开(公告)号：US20050199917A1

公开(公告)日：2005-09-15

申请号：US11057642

申请日：2005-02-14

申请人： Kyu-tae Yoo ,  Geun-bae Lim ,  Joon-ho Kim ,  Kak Namkoong

发明人： Kyu-tae Yoo ,  Geun-bae Lim ,  Joon-ho Kim ,  Kak Namkoong

IPC分类号： G01N27/327 ,  C12Q1/68 ,  G01N27/414 ,  G01N33/487 ,  H01L29/08

CPC分类号： G01N27/4145

摘要： There is provided a biomolecule FET enhancing a sensitivity. The biomolecule FET includes a substrate, first and second impurity regions formed on both sides of the substrate, and doped with impurities of a polarity opposite to that of the substrate, a gate formed on the substrate and being in contact with the first and second impurity regions, and a probe biomolecule attached to the gate. A region of the gate adjacent to the first impurity region is wider than a region thereof adjacent to the second impurity region. A density of the probe biomolecule attached to the surface of the gate is increased, and when detecting a level of hybridization of the probe biomolecule and the target biomolecule, its sensitivity is improved.

摘要翻译： 提供了提高灵敏度的生物分子FET。 生物分子FET包括基板，形成在基板两侧的第一和第二杂质区，并且掺杂极性与基板极性相反的杂质;形成在基板上并与第一和第二杂质接触的栅极 区域，以及连接到门的探针生物分子。 与第一杂质区相邻的栅极的区域比与第二杂质区相邻的区域宽。 附着在栅极表面的探针生物分子的密度增加，并且当检测到探针生物分子和靶生物分子的杂交水平时，其灵敏度得到改善。

公开(公告)号：US06877769B2

公开(公告)日：2005-04-12

申请号：US10326066

申请日：2002-12-23

申请人： Joon-Ho Kim ,  Ik-Hwan Kim ,  Byong-Ryong Cho ,  Gun-Woo Kim

发明人： Joon-Ho Kim ,  Ik-Hwan Kim ,  Byong-Ryong Cho ,  Gun-Woo Kim

IPC分类号： B60R21/213 ,  B60R21/16 ,  B60R21/231 ,  B60R21/26 ,  B60R21/22

CPC分类号： B60R21/232 ,  B60R2021/2612

摘要： A curtain air bag includes a diffuser having an opening that emits operation gas, placed at a predetermined area along an inner side of a roof rail, a first cushion divided into first and second areas that limits a front seat and a rear seat, a third area that forms a front area of the first area, and a fourth area placed between the first area and the second area. The curtain airbag further includes a plurality of cushion holders that fix the first cushion into the roof rail by coupling to the top of the first cushion, a first downwardly extended portion of the first cushion connected to a lower surface of the first area and providing a predetermined area to protect ribs of a passenger sitting in the front seat, a second downwardly extended portion of the first cushion connected to a lower part of the second area and providing a predetermined area to protect ribs of a passenger sitting in the rear seat.

摘要翻译： 帘式气囊包括具有排出操作气体的开口的扩散器，放置在沿着车顶轨道的内侧的预定区域，分为限定前排座椅和后座椅的第一和第二区域的第一坐垫，第三 区域，其形成第一区域的前区域，以及放置在第一区域和第二区域之间的第四区域。 帘式安全气囊还包括多个衬垫保持器，其通过联接到第一衬垫的顶部将第一衬垫固定到屋顶轨道中，第一衬垫的第一向下延伸部分连接到第一区域的下表面，并且提供 预定区域以保护坐在前座中的乘客的肋条，第一缓冲垫的第二向下延伸部分连接到第二区域的下部，并提供预定区域以保护坐在后座椅中的乘客的肋。

公开(公告)号：US06545938B2

公开(公告)日：2003-04-08

申请号：US09886465

申请日：2001-06-22

申请人： Byung Jae Lee ,  Joon Ho Kim ,  Young Jun Nam ,  Kwang Rae Cho ,  Sang Kwon Lee

发明人： Byung Jae Lee ,  Joon Ho Kim ,  Young Jun Nam ,  Kwang Rae Cho ,  Sang Kwon Lee

IPC分类号： G11C800

CPC分类号： G11C7/109 ,  G11C7/1078 ,  G11C7/1084 ,  G11C11/4082 ,  G11C2207/2227

摘要： A buffering circuit of a semiconductor memory device is provided with a plurality of buffers divided into groups, comprising: a first controller for generating a first enable signal in response to a refresh signal and a clock enable signal; a second controller for generating a second enable signal in response to an auto-refresh signal and the first enable signal; a first buffer block including at least one of signal input buffers controlled by the first enable signal; and a second buffer block including at least one of signal input buffers controlled by the second enable signal. The groups of the buffers are independently assigned to their corresponding enable signals.

公开(公告)号：US06256244B1

公开(公告)日：2001-07-03

申请号：US09605417

申请日：2000-06-27

申请人： Joon Ho Kim

发明人： Joon Ho Kim

IPC分类号： G11C700

CPC分类号： G11C11/406

摘要： Disclosed is a self refresh apparatus in a semiconductor memory device wherein sense amplifier connection signals are varied in logic level only when associated cell block selection signals vary in the self refresh mode, respectively. Accordingly, it is possible to reduce electric power consumed in a self refresh operation.

摘要翻译： 公开了一种半导体存储器件中的自刷新装置，其中只有当相关联的单元块选择信号分别在自刷新模式中变化时，读出放大器连接信号在逻辑电平中变化。 因此，可以减少自刷新操作中消耗的电力。

公开(公告)号：US5973521A

公开(公告)日：1999-10-26

申请号：US960228

申请日：1997-10-29

申请人： Jung Pill Kim ,  Joon Ho Kim

发明人： Jung Pill Kim ,  Joon Ho Kim

IPC分类号： H03K19/00 ,  H03K19/0185 ,  H03B1/00

CPC分类号： H03K19/0027 ,  H03K19/018585

摘要： A semiconductor device for automatically detecting an external interface voltage is disclosed, including a voltage converter for receiving an external interface voltage, and decreasing the external interface voltage value, and a voltage comparator for comparing the voltage outputted from the voltage converter with the internal supply voltage of a semiconductor chip. The semiconductor device automatically detects an external interface voltage environment where the semiconductor chip is used, and also automatically controls the magnitude of output buffer of the semiconductor chip according to the detected external interface voltage value, to prevent the operation speed from being deterioration.

摘要翻译： 公开了一种用于自动检测外部接口电压的半导体器件，包括用于接收外部接口电压的电压转换器和减小外部接口电压值;以及电压比较器，用于将从电压转换器输出的电压与内部电源电压进行比较 的半导体芯片。 半导体器件自动检测使用半导体芯片的外部接口电压环境，并且还根据检测到的外部接口电压值自动控制半导体芯片的输出缓冲器的大小，以防止操作速度劣化。

公开(公告)号：US09267618B2

公开(公告)日：2016-02-23

申请号：US13110452

申请日：2011-05-18

申请人： Chin-sung Park ,  Won-jong Jung ,  Jae-young Kim ,  Joon-ho Kim

发明人： Chin-sung Park ,  Won-jong Jung ,  Jae-young Kim ,  Joon-ho Kim

IPC分类号： B01L99/00 ,  F16K99/00 ,  B01L3/00

CPC分类号： F16K99/0059 ,  B01L3/502707 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0638 ,  F16K99/0001 ,  F16K99/0015 ,  F16K2099/008 ,  F16K2099/0084

摘要： A microvalve device comprises: a first substrate having a first surface, where at least one fluidic channel is formed into the first surface of the first substrate; and at least one valve seat is formed in the fluidic channel; a second substrate having a second surface, the second substrate comprising at least one air channel and at least one air chamber formed on the second surface of the second substrate, where the air chamber is connected to the air channel; and an elastic film interposed between the first substrate and the second substrate, where an upper portion of the valve seat is lower than the first surface of the first substrate.

摘要翻译： 微阀装置包括：具有第一表面的第一基板，其中至少一个流体通道形成在第一基板的第一表面中; 并且至少一个阀座形成在流体通道中; 具有第二表面的第二基板，所述第二基板包括至少一个空气通道和形成在所述第二基板的第二表面上的至少一个空气室，其中所述空气室连接到所述空气通道; 以及插入在第一基板和第二基板之间的弹性膜，其中阀座的上部比第一基板的第一表面低。