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公开(公告)号：US11155789B2
公开(公告)日：2021-10-26
申请号：US16278030
申请日：2019-02-15
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.
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公开(公告)号：US10815515B2
公开(公告)日：2020-10-27
申请号：US16145168
申请日：2018-09-28
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
IPC分类号： C12Q1/00 , C12N9/02 , C12Q1/32 , C12N9/04 , G01N27/327
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US10669565B2
公开(公告)日：2020-06-02
申请号：US16145190
申请日：2018-09-28
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190177763A1
公开(公告)日：2019-06-13
申请号：US15928027
申请日：2018-03-21
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12N9/02 , C12Q1/32
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190177700A1
公开(公告)日：2019-06-13
申请号：US16278025
申请日：2019-02-15
发明人： Hironori OMURA , Hirokazu SANDA , Takako YADA , Tetsunari MORITA , Mika KUYAMA , Tokuji IKEDA , Kenji KANO , Seiya TSUJIMURA
摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.
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公开(公告)号：US20190048383A1
公开(公告)日：2019-02-14
申请号：US16145166
申请日：2018-09-28
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
IPC分类号： C12Q1/00 , C12N9/02 , C12N9/04 , C12Q1/32 , G01N27/327
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190040443A1
公开(公告)日：2019-02-07
申请号：US16145190
申请日：2018-09-28
发明人： Hironori OMURA , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
IPC分类号： C12Q1/00 , C12N9/02 , C12N9/04 , C12Q1/32 , G01N27/327
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190017087A1
公开(公告)日：2019-01-17
申请号：US16145165
申请日：2018-09-28
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12N9/02 , C12Q1/32
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron accepter and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190017086A1
公开(公告)日：2019-01-17
申请号：US16145146
申请日：2018-09-27
发明人： Hironori OMURA , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12N9/02 , C12Q1/32
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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