Cell-free protein expression systems and methods of use thereof
    24.
    发明申请
    Cell-free protein expression systems and methods of use thereof 审中-公开
    无细胞蛋白质表达系统及其使用方法

    公开(公告)号:US20070128633A1

    公开(公告)日:2007-06-07

    申请号:US11546655

    申请日:2006-10-11

    CPC classification number: C12N9/1205 C12P21/02 C12Q1/485

    Abstract: Provided herein are in vitro (cell-free) protein translation (IVT) systems for the expression of kinases. In particular, provided herein is an IVT system for the expression of a panel of protein tyrosine kinases (PTK), (e.g., receptor protein tyrosine kinases (RTK) and/or cytoplasmic tyrosine kinases (CTK)), and/or fragments thereof (e.g., kinase domains and/or active fragments thereof).

    Abstract translation: 本文提供用于表达激酶的体外(无细胞)蛋白质翻译(IVT)系统。 特别地,本文提供的是用于表达蛋白酪氨酸激酶(PTK)(例如,受体蛋白酪氨酸激酶(RTK)和/或细胞质酪氨酸激酶(CTK))和/或其片段(例如, 例如,激酶结构域和/或其活性片段)。

    Detection of human T1R expressing cells
    26.
    发明授权
    Detection of human T1R expressing cells 有权
    人类T1R表达细胞的检测

    公开(公告)号:US09040247B2

    公开(公告)日:2015-05-26

    申请号:US13600377

    申请日:2012-08-31

    CPC classification number: C07K14/723 C07K14/705 C07K2319/00

    Abstract: Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular tastant in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed.

    Abstract translation: 描述了新鉴定的哺乳动物味细胞特异性G蛋白偶联受体,以及编码所述受体的基因和cDNA。 具体地说,描述了活跃于味觉信号传导中的T1R G蛋白偶联受体以及编码相同基因的cDNA,以及用于分离这些基因并用于分离和表达此类受体的方法。 还描述了用于表示哺乳动物中特定促味剂的味觉的方法,以及用于产生在哺乳动物中引发预定味觉的新颖分子或分子组合的方法,以及用于模拟一种或多种口味的方法。 此外,还公开了哺乳动物刺激或阻止味觉的方法。

    T1R3 nucleic acid fragments for use thereof as probes and for generating T1R specific antibodies
    29.
    发明授权
    T1R3 nucleic acid fragments for use thereof as probes and for generating T1R specific antibodies 有权
    T1R3核酸片段,用于探针和产生T1R特异性抗体

    公开(公告)号:US08247542B2

    公开(公告)日:2012-08-21

    申请号:US13087556

    申请日:2011-04-15

    CPC classification number: C07K14/723 C07K14/705 C07K2319/00

    Abstract: Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular tastant in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed.

    Abstract translation: 描述了新鉴定的哺乳动物味细胞特异性G蛋白偶联受体,以及编码所述受体的基因和cDNA。 具体地说,描述了活跃于味觉信号传导中的T1R G蛋白偶联受体以及编码相同基因的cDNA,以及用于分离这些基因并用于分离和表达此类受体的方法。 还描述了用于表示哺乳动物中特定促味剂的味觉的方法,以及用于产生在哺乳动物中引发预定味觉的新颖分子或分子组合的方法,以及用于模拟一种或多种口味的方法。 此外,还公开了哺乳动物刺激或阻止味觉的方法。

    Human T1R2 taste receptor nucleic acids and cells containing these nucleic acids
    30.
    发明授权
    Human T1R2 taste receptor nucleic acids and cells containing these nucleic acids 有权
    人类T1R2味觉受体核酸和含有这些核酸的细胞

    公开(公告)号:US08153774B2

    公开(公告)日:2012-04-10

    申请号:US12436886

    申请日:2009-05-07

    Abstract: Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular taste stimulus in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed.

    Abstract translation: 描述了新鉴定的哺乳动物味细胞特异性G蛋白偶联受体,以及编码所述受体的基因和cDNA。 具体地说,描述了活跃于味觉信号传导中的T1R G蛋白偶联受体以及编码相同基因的cDNA,以及用于分离这些基因并用于分离和表达此类受体的方法。 还描述了用于表示哺乳动物中特定味觉刺激的味觉感知的方法,以及用于产生哺乳动物中引发预定味觉的新颖分子或分子组合的方法,以及用于模拟一种或多种口味的方法。 此外,还公开了哺乳动物刺激或阻止味觉的方法。

Patent Agency Ranking