公开(公告)号：US20120156678A1

公开(公告)日：2012-06-21

申请号：US13392940

申请日：2010-08-30

Applicant: Xiaochun Le ,  Hongquan Zhang ,  Xing-Fang Li

Inventor： Xiaochun Le ,  Hongquan Zhang ,  Xing-Fang Li

IPC: G01N33/53 ,  C12Q1/68

CPC classification number: G01N33/53 ,  C12Q1/6816 ,  G01N33/542 ,  G01N2458/10 ,  C12Q2525/301

Abstract: This application relates to a binding-induced hairpin detection system comprising: a. a first probe comprising a targeting molecule and an oligonucleotide that has a free end and an end attached to the targeting molecule; and b. a second probe comprising a targeting molecule and an oligonucleotide that has an end attached to the targeting molecule and a free end comprising a nucleotide sequence that is complementary to a nucleotide sequence at or near the free end of the oligonucleotide of the first probe; wherein upon binding of the targeting molecule to a target molecule, the free end of the oligonucleotide of the second probe hybridizes at or near the free end of the oligonucleotide of the first probe forming a hairpin stem, the non-hybridized portions of the first and second probes together with the target molecule bound thereto forming a hairpin loop, thereby providing a binding-induced hairpin.

Abstract translation: 本申请涉及绑定诱导的发夹检测系统，包括：a。 第一探针，其包含靶向分子和寡核苷酸，所述寡核苷酸具有连接于所述靶向分子的游离末端和末端; 和b。 第二探针，其包含靶向分子和具有连接于靶向分子的末端的寡核苷酸，以及包含与第一探针的寡核苷酸的游离末端或其附近的核苷酸序列互补的核苷酸序列的游离末端; 其中当靶向分子与靶分子结合时，第二探针的寡核苷酸的游离末端在形成发夹茎的第一探针的寡核苷酸的自由端或其附近杂交，第一和第二探针的非杂交部分 第二探针与结合的靶分子一起形成发夹环，从而提供结合诱导的发夹。