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30 条记录 (耗时 0.034 秒)

    Nucleotide sequences for encoding of the lysR2-gene
    23.
    发明授权
    Nucleotide sequences for encoding of the lysR2-gene 失效
    编码lysR2基因的核苷酸序列

    公开(公告)号：US07078502B2

    公开(公告)日：2006-07-18

    申请号：US09826909

    申请日：2001-04-06

    申请人： Bettina Moeckel ,  Mike Farwick ,  Thomas Hermann ,  Caroline Kreutzer ,  Walter Pfefferle

    发明人： Bettina Moeckel ,  Mike Farwick ,  Thomas Hermann ,  Caroline Kreutzer ,  Walter Pfefferle

    IPC分类号： C07H21/02 ,  C07H21/04 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63

    CPC分类号： C12P13/08 ,  C07K14/34

    摘要： Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.

    摘要翻译： 分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸，b）多核苷酸， 其编码多肽，其包含与SEQ ID No.2的氨基酸序列至少70％的程度相同的氨基酸序列，c）与a）或b）的多核苷酸互补的多核苷酸， 和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少lysR2基因存在于减毒 形式，以及使用多核苷酸序列作为杂交探针。

    Attenuated CCPA1 modified bacterial cell and its use for the preparation of L-amino acids
    25.
    发明授权
    Attenuated CCPA1 modified bacterial cell and its use for the preparation of L-amino acids 有权
    减毒CCPA1修饰细菌细胞及其用于制备L-氨基酸的用途

    公开(公告)号：US07101690B2

    公开(公告)日：2006-09-05

    申请号：US10895849

    申请日：2004-07-22

    申请人： Bettina Moeckel ,  Caroline Kreutzer

    发明人： Bettina Moeckel ,  Caroline Kreutzer

    IPC分类号： C12P13/04 ,  C12N1/20 ,  C12N15/00 ,  C07K1/00 ,  C07H21/02

    CPC分类号： C07K14/34 ,  C12P13/08

    摘要： The present invention provides isolated polynucleotides containing a polynucleotide sequence which is: a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, or b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, or c) polynucleotide which is complementary to the polynucleotides of a) or b), or d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the ccpA1 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.

    摘要翻译： 本发明提供了包含多核苷酸序列的分离的多核苷酸，其是：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸，或b ）多核苷酸，其编码多肽，其包含与SEQ ID No.2的氨基酸序列至少70％的程度相同的氨基酸序列，或c）与a）或 b）或d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少所述ccpA1基因是 以减毒形式存在，以及使用多核苷酸序列作为杂交探针。

    Nucleotide sequences coding for the ccpA1 gene
    26.
    发明申请
    Nucleotide sequences coding for the ccpA1 gene 有权
    编码ccpA1基因的核苷酸序列

    公开(公告)号：US20050032179A1

    公开(公告)日：2005-02-10

    申请号：US10895849

    申请日：2004-07-22

    申请人： Bettina Moeckel ,  Caroline Kreutzer

    发明人： Bettina Moeckel ,  Caroline Kreutzer

    IPC分类号： C07K14/34 ,  C12N1/21 ,  C12P13/08 ,  C07H21/04 ,  C12N9/10 ,  C12N15/74

    CPC分类号： C07K14/34 ,  C12P13/08

    摘要： The present invention provides isolated polynucleotides containing a polynucleotide sequence which is: a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, or b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, or c) polynucleotide which is complementary to the polynucleotides of a) or b), or d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the ccpA1 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.

    摘要翻译： 本发明提供了包含多核苷酸序列的分离的多核苷酸，其是：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸，或b ）多核苷酸，其编码多肽，其包含与SEQ ID No.2的氨基酸序列至少70％的程度相同的氨基酸序列，或c）与a）或 b）或d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少所述ccpA1基因是 以减毒形式存在，以及使用多核苷酸序列作为杂交探针。

    Nucleotide sequences which code for the lysR3 gene
    27.
    发明授权
    Nucleotide sequences which code for the lysR3 gene 失效
    编码lysR3基因的核苷酸序列

    公开(公告)号：US06812006B2

    公开(公告)日：2004-11-02

    申请号：US09867537

    申请日：2001-12-11

    申请人： Bettina Moeckel ,  Caroline Kreutzer

    发明人： Bettina Moeckel ,  Caroline Kreutzer

    IPC分类号： C12P1304

    CPC分类号： C12P13/08 ,  C07K14/34

    摘要： The present invention relates to polynucleotides corresponding to the lysR3 gene and which encode a LysR3 transcriptional regulator, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having LysR3 transcriptional regulator activity.

    摘要翻译： 本发明涉及对应于lysR3基因并编码LysR3转录调节剂的多核苷酸，产生L-氨基酸的方法，以及筛选编码具有LysR3转录调节活性的蛋白质的多核苷酸的方法。

    Nucleotide sequences coding for the ccpA1 gene
    29.
    发明授权
    Nucleotide sequences coding for the ccpA1 gene 失效
    编码ccpA1基因的核苷酸序列

    公开(公告)号：US06838267B2

    公开(公告)日：2005-01-04

    申请号：US09938540

    申请日：2001-08-27

    申请人： Bettina Moeckel ,  Caroline Kreutzer

    发明人： Bettina Moeckel ,  Caroline Kreutzer

    IPC分类号： C07K14/34 ,  C12N1/21 ,  C12P13/08 ,  C12P13/04

    CPC分类号： C07K14/34 ,  C12P13/08

    摘要： The present invention provides isolated polynucleotides containing a polynucleotide sequence which is: a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, or b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, or c) polynucleotide which is complementary to the polynucleotides of a) or b), or d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the ccpA1 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.

    Production of L-isoleucine by means of recombinant microorganisms with deregulated threonine dehydratase
    30.
    发明授权
    Production of L-isoleucine by means of recombinant microorganisms with deregulated threonine dehydratase 失效
    通过具有失调的苏氨酸脱水酶的重组微生物生产L-异亮氨酸

    公开(公告)号：US6107063A

    公开(公告)日：2000-08-22

    申请号：US669378

    申请日：1997-03-20

    申请人： Bettina Moeckel ,  Lothar Eggeling ,  Hermann Sahm

    发明人： Bettina Moeckel ,  Lothar Eggeling ,  Hermann Sahm

    IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/88 ,  C12N15/00 ,  C12N15/60 ,  C12P13/06 ,  C12R1/15 ,  C12N15/31 ,  C12N15/63

    CPC分类号： C12N9/88 ,  C12P13/06

    摘要： The invention relates to processes for the microbial production of L-isoleucine. To this end, in a gene in vitro of a threonine dehydratse, one or more bases in the gene region coding the enzyme's allosteric domains are exchanged in such a way that at least one amino acid in the amino acid sequence of the allosteric domains of the enzyme is replaced by another so that the enzyme is no longer inhibited by L-isoleucine feedback. Furthermore, concrete amino acid exchanges in the amino acid sequence of the enzyme are effected in a gene in vitro of a threonin dehydratase of Corynebacterium glutamicum by base exchange both outside and inside and outside the gene region coding the allosteric domains of the enzyme si that, after the transformation of such mutated threonine dehydratase genes into a threonine or L-isoleucine-producing host cell, the latter repeatedly forms L-isoleucine.

    摘要翻译： PCT No.PCT / DE95 / 00017 Sec。 371日期1997年3月20日 102（e）1997年3月20日PCT PCT 1995年1月9日PCT公布。 WO95 / 19442 PCT公开号 日期1995年7月20日本发明涉及L-异亮氨酸的微生物生产方法。 为此，在苏氨酸脱水体外的基因的基因中，编码酶变构域的基因区域中的一个或多个碱基以这样的方式交换，使得至少一个氨基酸序列的变构域 酶被另一个替代，使得酶不再被L-异亮氨酸反馈抑制。 此外，酶的氨基酸序列中的具体氨基酸交换通过在编码酶si的变构结构域的基因区域的外部和外部的碱基交换体外在谷氨酸棒杆菌的苏氨酸脱水酶的基因中进行， 在将这种突变的苏氨​​酸脱水酶基因转化成产生苏氨酸或L-异亮氨酸的宿主细胞后，后者重复形成L-异亮氨酸。