公开(公告)号：US11352622B2

公开(公告)日：2022-06-07

申请号：US16667446

申请日：2019-10-29

Applicant: Cepheid

Inventor： Russell Higuchi

IPC: C12Q1/68 ,  C12N15/11 ,  C12P19/34 ,  C12Q1/6876

Abstract: Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Modified bases can be employed in primers to provide this base-3 or greater amplification with satisfactory PCR cycle times, which are improved, as compared to those observed in the absence of modified bases.

公开(公告)号：US20190203266A1

公开(公告)日：2019-07-04

申请号：US16359947

申请日：2019-03-20

Applicant: Cepheid

Inventor： Russell Higuchi

IPC: C12Q1/686

CPC classification number: C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/191 ,  C12Q2531/119

Abstract: Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR.

公开(公告)号：US10273534B2

公开(公告)日：2019-04-30

申请号：US15535009

申请日：2015-12-15

Applicant: CEPHEID

Inventor： Russell Higuchi

IPC: C12Q1/68 ,  C12Q1/686

Abstract: Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR.

公开(公告)号：US10202653B2

公开(公告)日：2019-02-12

申请号：US14394352

申请日：2013-04-19

Applicant: Cepheid

Inventor： Russell Higuchi ,  Stacey Ellen Wallace ,  Edwin Wei-Lung Lai

IPC: C12Q1/68 ,  C12Q1/6886

Abstract: Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting a set of markers consisting of CRH, IGF2, KRT20, and ANXA10.

公开(公告)号：US20150376683A1

公开(公告)日：2015-12-31

申请号：US14402679

申请日：2013-05-22

Applicant: CEPHEID ,  JAMES WANG

Inventor： Alfhous Weir ,  David Persing ,  Russell Higuchi ,  James Wang ,  Reuel VanAtta ,  William E. Murray

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12N15/111 ,  C12N2310/141 ,  C12N2320/10 ,  C12Q1/6883 ,  C12Q2600/112 ,  C12Q2600/156

Abstract: Compositions and methods for detecting Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are provided. The present invention also provides methods and compositions for screening for infection/inflammation based on genomic copy number. Described herein is a method that entails assaying a sample obtained from the urogenital tract of the mammal for an indicator of genomic copy number, wherein a genomic copy number level that is higher than a control genomic copy number level is indicative of the presence of infection or inflammation of the urogenital tract. Also described in a kit of the invention that includes a primer and/or probe for detecting or sequencing an indicator of genomic copy number, wherein the indicator of genomic copy number comprises a nucleic acid sequence that is expected to be present in the genome of the mammal in one or two copies; and a primer and/or probe for detecting or sequencing a nucleic acid sequence that is indicative of a pathogen that infects the urogenital tract or a miRNA correlated with inflammation.

Abstract translation: 提供了检测沙眼衣原体（CT）和淋病奈瑟氏球菌（NG）的组合物和方法。 本发明还提供了基于基因组拷贝数筛选感染/炎症的方法和组合物。 本文描述了一种方法，其需要测定从哺乳动物的泌尿生殖道获得的样品用于基因组拷贝数的指标，其中高于对照基因组拷贝数水平的基因组拷贝数水平指示感染的存在或 泌尿生殖道炎症。 还在本发明的试剂盒中描述，其包括用于检测或测序基因组拷贝数指标的引物和/或探针，其中基因组拷贝数的指示符包含预期存在于基因组拷贝数的基因组中的核酸序列 哺乳动物一至二份; 以及用于检测或测序指示感染泌尿生殖道的病原体的核酸序列或与炎症相关的miRNA的引物和/或探针。