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43 条记录 (耗时 0.026 秒)

    Nephrin gene and protein
    22.
    发明申请
    Nephrin gene and protein 有权
    Nephrin基因和蛋白质

    公开(公告)号：US20070009952A1

    公开(公告)日：2007-01-11

    申请号：US11519314

    申请日：2006-09-12

    申请人： Karl Tryggvason ,  Marjo Kestila ,  Ulla Lenkkeri ,  Minna Mannikko

    发明人： Karl Tryggvason ,  Marjo Kestila ,  Ulla Lenkkeri ,  Minna Mannikko

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6883 ,  A61K38/00 ,  A61K48/00 ,  C07K14/70525 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/172 ,  Y10S435/81

    摘要： The present invention provides for compositions and methods for detecting susceptibility for basement membrane disease, in particular congenital nephrotic syndromes of the Finnish type. The present invention provides for nucleic acids and protein for use in methods and compositions for the diagnosis of disease and identification of small molecule therapeutics for treatment of such disease, in particular of proteinuria associated with kidney disease.

    摘要翻译： 本发明提供用于检测基底膜疾病，特别是芬兰型先天性肾病综合征的易感性的组合物和方法。 本发明提供用于诊断疾病的方法和组合物中的核酸和蛋白质，以及用于治疗这种疾病，特别是与肾脏疾病相关的蛋白尿的小分子治疗剂的鉴定。

    Isolated DNA sequence for a novel macrophage receptor with a collagenous domain
    23.
    发明授权
    Isolated DNA sequence for a novel macrophage receptor with a collagenous domain 失效
    用于具有胶原结构域的新型巨噬细胞受体的分离的DNA序列

    公开(公告)号：US5691197A

    公开(公告)日：1997-11-25

    申请号：US392367

    申请日：1995-02-21

    申请人： Karl Tryggvason ,  Outi Elomaa ,  Maarit Kangas

    发明人： Karl Tryggvason ,  Outi Elomaa ,  Maarit Kangas

    IPC分类号： C07K14/705 ,  C12N15/12 ,  C12N15/85

    CPC分类号： C07K14/705

    摘要： The present invention is directed to processes for isolating and identifying the nucleotide sequence of a gene for a novel macrophage receptor with collagenous structure, termed "MARCO". The new macrophage receptor with a collagenous domain binds gram positive and negative bacteria and acetylated LDL. Moreover, the invention relates to the nucleotide sequence for MARCO identified by the process of the invention and the isolated and purified polypeptide chain encoded by such a sequence.

    摘要翻译： 本发明涉及用于分离和鉴定具有胶原结构的新型巨噬细胞受体的基因的核苷酸序列的方法，称为“MARCO”。 具有胶原结构域的新型巨噬细胞受体结合革兰氏阳性和阴性细菌和乙酰化LDL。 此外，本发明涉及通过本发明的方法鉴定的MARCO的核苷酸序列和由这种序列编码的分离和纯化的多肽链。

    Immunological methods for the detection of the human type IV collagen .alpha.5 chain
    25.
    发明授权
    Immunological methods for the detection of the human type IV collagen .alpha.5 chain 失效
    用于检测人类IV型胶原α5链的免疫学方法

    公开(公告)号：US5593900A

    公开(公告)日：1997-01-14

    申请号：US321084

    申请日：1994-10-11

    申请人： Karl Tryggvason ,  Sirkka L. Hostikka ,  Matti Hoyhtya

    发明人： Karl Tryggvason ,  Sirkka L. Hostikka ,  Matti Hoyhtya

    IPC分类号： C07K14/78 ,  C12Q1/68 ,  C12P21/00 ,  C07K16/18

    CPC分类号： C12Q1/6883 ,  C07K14/78

    摘要： The present invention provides for a method for preparing antibodies that specifically detect the type IV collagen .alpha.5 chain that is defective in various basement membrane disorders such as in X chromosome-linked Alport's syndrome. The invention further provides for the use of such antibodies to detect the .alpha.5(IV) collagen chain in solutions and other human tissue specimens using immunological methods comprised of antibodies specific for said protein. Along this line, the invention relates to the use of the specific antibodies to examine the presence or absence of the .alpha.5(IV) collagen chain in the tissues, e.g. skin or kidneys, etc. of patients with renal failure, possibly due to Alport's syndrome. In addition, a further object of the present invention is to provide compositions, comprising both labeled and unlabeled peptides containing sequences from the .alpha.5(IV) chain antigen (either as single peptides or conjugated to a carrier molecule or matrix) or labeled or unlabeled antibodies to said .alpha.5(IV) collagen chain or peptides containing sequence specific for the said polypeptide chain, and the use of the compositions for the diagnosis of basement membrane disorders, such as Alport's syndrome.

    摘要翻译： 本发明提供了一种制备抗体的方法，所述抗体特异性检测了各种基底膜病症如X染色体连锁的阿尔波特综合征中有缺陷的IV型胶原α5链。 本发明进一步提供了使用这种抗体以使用由所述蛋白质特异性抗体组成的免疫学方法来检测溶液和其它人体组织标本中的α5（IV）胶原链。 沿着这条线，本发明涉及特异性抗体用于检查组织中α5（IV）胶原链的存在或不存在的情况，例如， 肾衰竭患者的皮肤或肾脏等，可能是由于阿尔波特综合症。 此外，本发明的另一个目的是提供包含标记的和未标记的肽的组合物，其含有来自α5（IV）链抗原的序列（作为单一肽或与载体分子或基质缀合）或标记或未标记 对所述α5（IV）胶原链或含有针对所述多肽链特异性序列的肽的抗体，以及所述组合物用于诊断基底膜疾病如阿尔波特综合征的用途。

    Composition and method for enabling proliferation of pluripotent stem cells
    28.
    发明授权
    Composition and method for enabling proliferation of pluripotent stem cells 有权
    使多能干细胞增殖的组成和方法

    公开(公告)号：US08951799B2

    公开(公告)日：2015-02-10

    申请号：US12583750

    申请日：2009-08-25

    申请人： Anna Domogatskaya ,  Sergey Rodin ,  Karl Tryggvason

    发明人： Anna Domogatskaya ,  Sergey Rodin ,  Karl Tryggvason

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/07 ,  C12N5/10 ,  C12N5/071 ,  C12N5/0735

    CPC分类号： C12N5/0606 ,  C12N2533/52

    摘要： The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, that both maintain stem cells in vitro pluripotency and enable self-renewal. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or their functional domains, in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferated and maintained their pluripotency.

    摘要翻译： 本公开涉及组合物的发展，例如细胞外基质及其使用方法，其既维持干细胞体外多能性并且实现自我更新。 在这方面，已经发现，当在不存在分化抑制剂或饲养细胞的情况下，将多能小鼠和人胚胎干细胞培养在用重组层粘连蛋白-10（laminin-511）或其功能结构域包被的平板上时，胚胎干 细胞增殖并维持其多能性。

    Recombinant laminin-521
    29.
    发明授权
    Recombinant laminin-521 有权
    重组层粘连蛋白-521

    公开(公告)号：US08415156B2

    公开(公告)日：2013-04-09

    申请号：US13328145

    申请日：2011-12-16

    申请人： Karl Tryggvason ,  Sergey Rodin

    发明人： Karl Tryggvason ,  Sergey Rodin

    IPC分类号： C12N5/00 ,  A01N63/00

    CPC分类号： C07K14/78 ,  C12N5/0606 ,  C12N5/0696 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2501/608 ,  C12N2510/00 ,  C12N2533/52 ,  Y10T428/13

    摘要： The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with a matrix of recombinant laminin-521 (laminin 11), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein.

    摘要翻译： 本公开涉及分离的层粘连蛋白-521，制备重组层粘连蛋白521的方法，表达重组层粘连蛋白-521的宿主细胞和含有层粘连蛋白-51的组合物。 层粘连蛋白-521可以维持干细胞的体外多能性，实现自我更新，并能使人类胚胎干细胞的单细胞存活。 当多能人胚胎干细胞在涂有重组层粘连蛋白-51（层粘连蛋白11）基质的平板上培养时，在不存在分化抑制剂或饲养细胞的情况下，胚胎干细胞增殖并保持其多能性。 还已经发现人重组层粘连蛋白-51（层粘连蛋白-11）在完全解离成单细胞悬液后提供干细胞的单细胞存活。 含有至多3.9ng / ml的β成纤维细胞生长因子（bFGF）的有用细胞培养基也在本文中描述。

    ISOLATED LAMININ-421
    30.
    发明申请
    ISOLATED LAMININ-421 有权
    隔离的LAMININ-421

    公开(公告)号：US20120302512A1

    公开(公告)日：2012-11-29

    申请号：US13409594

    申请日：2012-03-01

    申请人： Karl Tryggvason ,  Sergey Rodin

    发明人： Karl Tryggvason ,  Sergey Rodin

    IPC分类号： C07K14/435 ,  C12P21/02 ,  A61K38/14

    CPC分类号： C07K14/78 ,  A61K38/00 ,  C07K2319/033 ,  C07K2319/036 ,  C07K2319/09 ,  C07K2319/41 ,  C07K2319/43

    摘要： The present disclosure provides isolated laminin-421, methods for making recombinant laminin-421, and host cells that express recombinant laminin-421. The present disclosure also provides nucleic acid sequences encoding full length human laminin β2 chain, expression vectors and host cells thereof.

    摘要翻译： 本公开提供分离的层粘连蛋白421，用于制备重组层粘连蛋白421的方法和表达重组层粘连蛋白421的宿主细胞。 本公开还提供了编码全长人层粘连蛋白和其2链，表达载体和宿主细胞的核酸序列。