公开(公告)号：US20070057180A1

公开(公告)日：2007-03-15

申请号：US11224093

申请日：2005-09-13

申请人： Stuart Hansen ,  Gangqiang Li ,  Gavin Reid

发明人： Stuart Hansen ,  Gangqiang Li ,  Gavin Reid

IPC分类号： H01J49/42

CPC分类号： H01J49/4225

摘要： A method and apparatus for processing ions in mass spectrometry is provided. The apparatus includes an ion processing cell having segmented multipole rods and a means for admitting reactive reagent ions to the cell. Provision for timed sequence control of RF and DC voltages to the segmented multipole rods is included. Processing includes trapping analyte ions and subjecting them to selected reactions with reactive reagent ions and/or physical manipulation of the ions by changing the characteristics of the electric field and/or creating discrete regions in the field.

摘要翻译： 提供了一种在质谱法中处理离子的方法和装置。 该装置包括具有分段多极棒的离子处理单元和用于将反应性试剂离子引入该单元的装置。 包括对分段多极杆的RF和DC电压的定时序列控制。 处理包括捕获分析物离子并使其与反应性试剂离子进行选择的反应和/或通过改变电场的特性和/或在场中产生离散区域来离子的物理操作。

公开(公告)号：US06994960B1

公开(公告)日：2006-02-07

申请号：US09424629

申请日：1998-05-28

申请人： Simon Foote ,  Colleen Elso ,  Richard Simpson ,  Gavin Reid

发明人： Simon Foote ,  Colleen Elso ,  Richard Simpson ,  Gavin Reid

IPC分类号： C12Q1/68 ,  C12N15/00 ,  C12N15/63 ,  C12N1/20 ,  C07H21/04

CPC分类号： C12Q1/683 ,  C12Q2565/627

摘要： A method of detecting a mutation or a difference of one or more nucleotides between a nucleic acid molecule to be tested and a reference nucleic acid molecule, said method comprising subjecting the test nucleic acid molecule to base specific cleavage to generate oligonucleotide fragments, separating the resulting oligonucleotide fragments based on mass by MALDI-ATOF MS and/or other equivalent procedure to produce a fingerprint of then oligonucleotide fragments comprising one or more peaks wherein a peak represents the mass of each fragment and identifying an altered peak relative to a reference nucleic acid molecule subjected to the same procedure wherein the presence of an altered peak is indicative of a difference of one or more nucleotides in said tested nucleic acid molecule.

摘要翻译： 一种检测待测核酸分子与参考核酸分子之间的一个或多个核苷酸的突变或差异的方法，所述方法包括使测试核酸分子对特异性切割进行碱基产生寡核苷酸片段，分离所得的 基于质量的基于质粒的寡核苷酸片段通过MALDI-ATOF MS和/或其他等同方法产生包含一个或多个峰的寡核苷酸片段的指纹，其中峰表示每个片段的质量，并鉴定相对于参照核酸分子的改变的峰 进行相同的程序，其中改变的峰的存在指示所述测试的核酸分子中一个或多个核苷酸的差异。

公开(公告)号：US20050221500A1

公开(公告)日：2005-10-06

申请号：US10514693

申请日：2003-05-20

申请人： Gavin Reid ,  Jason Hogan ,  Scott McLuckey

发明人： Gavin Reid ,  Jason Hogan ,  Scott McLuckey

IPC分类号： B01D59/44 ,  C07K16/00 ,  C12Q1/00 ,  G01N20060101 ,  G01N1/00 ,  G01N24/00 ,  G01N33/48 ,  G01N33/50 ,  G01N33/68 ,  G06F19/00

CPC分类号： G01N33/6848 ,  H01J49/00

摘要： Mass spectrometry is used to identify a protein of interest. The protein is first ionized then fragmented into protein product ion. Masses of the observed product ions are compared to product ion masses calculated in silico for database protein sequences to identify product ion matches within a predetermined mass tolerance. An algorithm that weights the product ion to matches based upon one or more factors such as product ion abundance, favored cleavage sites, product ion type, precursor ion charge state and polarity is used to score the possible matches to database proteins in order to identify the protein of interest. The invention represents a “top down” approach and is particularly well-suited for identification of a protein in a complex mixture.

摘要翻译： 质谱用于鉴定感兴趣的蛋白质。 蛋白质首先被电离，然后分裂成蛋白质产物离子。 将观察到的产物离子的质量与对于数据库蛋白质序列计算的产物离子质量进行比较，以在预定质量公差内鉴定产物离子匹配。 使用基于一个或多个因素（例如产物离子丰度，有利的切割位点，产物离子类型，前体离子电荷状态和极性）对产物离子进行匹配的算法来评估与数据库蛋白质的可能匹配，以便鉴定 感兴趣的蛋白质 本发明代表“自上而下”的方法，并且特别适合于鉴定复杂混合物中的蛋白质。