公开(公告)号：US20210002704A1

公开(公告)日：2021-01-07

申请号：US17025735

申请日：2020-09-18

Applicant: Gen-Probe Incorporated

Inventor： David Buse ,  David Howard Combs ,  Norbert D. Hagen ,  David Opalsky ,  Bruce Richardson ,  Anita Prasad ,  Keith Moravick ,  Tyler Moore ,  Byron J. Knight

IPC: C12Q1/686 ,  B01L7/00 ,  G01N21/64

Abstract: Receptacles containing a reaction mixture are transported to receptacle wells of a thermally conductive receptacle holder, and the temperature of the holder is cycled with a thermoelectric device situated between a support and a first side of the holder while a first force is applied onto a second side of the holder. Optical communication between each well and an excitation signal source and an emission signal detector is established to determine whether an emission signal is emitted from any of the receptacles during temperature cycling as an indication of the presence of a target nucleic acid. The thermoelectric device may be situated between an upright portion of the support and the first side of the holder. A second force may be applied onto a top end of each receptacle in the holder by a cover moveable with respect to the receptacles between an open position and a closed position.

公开(公告)号：US20190284614A1

公开(公告)日：2019-09-19

申请号：US16434910

申请日：2019-06-07

Applicant: Gen-Probe Incorporated

Inventor： David BUSE ,  David Howard Combs ,  Norbert D. Hagen ,  David Opalsky ,  Bruce Richardson ,  Anita Prasad ,  Keith Moravick ,  Tyler Moore

IPC: C12Q1/686 ,  G01N21/64 ,  B01L7/00

Abstract: A thermally conductive receptacle holder includes a plurality of wells that each receive a receptacle. A cross-brace is mounted to a support to exert a force onto an outer surface of a compressive housing such that the compressive housing exerts a force onto the receptacle holder. An optical fiber associated with each well provides optical communication between the receptacle well and an excitation signal source and/or an emission signal detector.