公开(公告)号：US20080239311A1

公开(公告)日：2008-10-02

申请号：US12051245

申请日：2008-03-19

申请人： Tsuyoshi SONEHARA ,  Takashi Anazawa ,  Kenko Uchida ,  Tomoyuki Sakai

发明人： Tsuyoshi SONEHARA ,  Takashi Anazawa ,  Kenko Uchida ,  Tomoyuki Sakai

IPC分类号： G01J3/30 ,  G02B5/08

CPC分类号： G01N21/6452 ,  G02B2207/113

摘要： In order to provide a fluorescence detection apparatus having a high sensitivity, a high processing capacity and a competitive edge in cost, the fluorescence detection apparatus according to this invention irradiate the sample with light so that the aspect ratio of the form of the irradiated region by light on the arrangement surface of the sample may be 1±0.1. The preferable form of irradiate region is not limited to one and varies to some extent depending on the item to be optimized. The form of irradiated region may be, for example, a circle, an equilateral triangle, a square, a regular hexagon and the like.

摘要翻译： 为了提供具有高灵敏度，高处理能力和成本方面的竞争优势的荧光检测装置，根据本发明的荧光检测装置用光照射样品，使得照射区域的形式的纵横比由 样品排列表面上的光可能为1±0.1。 照射区域的优选形式不限于一个，并且根据要优化的项目而在一定程度上变化。 照射区域的形式可以是例如圆形，等边三角形，正方形，正六边形等。

公开(公告)号：US5523058A

公开(公告)日：1996-06-04

申请号：US240733

申请日：1994-05-12

申请人： Shinichiro Umemura ,  Kenichi Kawabata ,  Kenko Uchida ,  Kenji Yasuda ,  Yasuo Wada ,  Atsushi Hiraiwa

发明人： Shinichiro Umemura ,  Kenichi Kawabata ,  Kenko Uchida ,  Kenji Yasuda ,  Yasuo Wada ,  Atsushi Hiraiwa

IPC分类号： A61B17/22 ,  A61N7/00 ,  A61N7/02 ,  B01F11/02 ,  A61B8/00 ,  B06B1/00

CPC分类号： A61N7/02 ,  A61B17/22004 ,  A61N2007/006 ,  A61N2007/0078 ,  A61N2007/0095

摘要： By providing an ultrasonic irradiation apparatus for generating acoustic cavitation efficiently, it is intended to realize an ultrasonic therapeutic apparatus for generating the action of cavitation on a living body suitable for medical treatment of malignant tumors and medical treatment of thrombi and calculi, an ultrasonic diagnostic apparatus for generating cavitation for emphasizing an ultrasonic echo image such as a blood flow and utilizing the reflection capability of the cavitation, an ultrasonic chemical reaction accelerating apparatus, an ultrasonic cleaning apparatus or an ultrasonic sterilizing apparatus. Irradiation focus/code signals for defining irradiated acoustic fields of a fundamental wave and a second harmonic wave as well as focus positions/acoustic pressure distribution forms of the respective waves are applied from an irradiation unit main control circuit to drive phase generating circuits. Generated drive phases are applied to drive signal generating circuits, generated drive signals are applied to element drive circuits and a group of fundamental frequency elements and a group of second harmonic elements are driven. The drive phases are controlled such that the fundamental wave and second harmonic wave are superimposed on each other in a medium near a focal point, thus generating acoustic caviation locally and efficiently.

摘要翻译： PCT No.PCT / JP93 / 01310 Sec。 371日期：1994年5月12日 102（e）日期1994年5月12日PCT提交1993年9月14日PCT公布。 公开号WO94 / 06380 日期为1994年3月31日。通过提供用于有效产生声孔的超声波照射装置，旨在实现用于产生适于恶性肿瘤治疗的生物体上的空化作用的超声波治疗装置和血栓的治疗 和结石，用于产生用于强调超声波回波图像如血流并利用空化反射能力的超声波诊断装置，超声波化学反应加速装置，超声波清洗装置或超声波消毒装置。 用于定义基波和二次谐波的辐射声场以及各波的聚焦位置/声压分布形式的照射焦点/码信号从照射单元主控制电路施加到驱动相位产生电路。 产生的驱动相位被施加到驱动信号发生电路，所产生的驱动信号被施加到元件驱动电路，并且一组基频元件和一组二次谐波元件被驱动。 控制驱动相位使得基波和二次谐波在焦点附近的介质中彼此叠加，从而局部且有效地产生声空化。

公开(公告)号：US5183744A

公开(公告)日：1993-02-02

申请号：US425028

申请日：1989-10-23

申请人： Yoshio Kawamura ,  Shinji Tanaka ,  Kazuo Sato ,  Kenko Uchida ,  Hiroyuki Kohida

发明人： Yoshio Kawamura ,  Shinji Tanaka ,  Kazuo Sato ,  Kenko Uchida ,  Hiroyuki Kohida

IPC分类号： C12M1/32 ,  C12M3/00 ,  C12N13/00 ,  C12Q1/24

CPC分类号： C12Q1/24 ,  C12M35/02 ,  C12N13/00

摘要： A method and an apparatus for the treatment of particles or biological cells are disclosed, wherein the particles or cells are precipitated or ascended depending on the specific gravity of a liquid used, and the particles or cells are handled with the aid of a holding plate. A method and an apparatus for the fusion of particles or cells are also disclosed, wherein an electric voltage is loaded on a position between electrodes in a microchamber to fuse the particles or cells held in the microchamber.

摘要翻译： 公开了一种用于处理颗粒或生物细胞的方法和装置，其中所述颗粒或细胞根据所用液体的比重而沉淀或上升，并且借助于保持板处理颗粒或细胞。 还公开了一种用于熔化颗粒或细胞的方法和装置，其中电压被加载在微室中的电极之间的位置，以熔化保持在微室中的颗粒或细胞。

公开(公告)号：US20100167281A1

公开(公告)日：2010-07-01

申请号：US12389151

申请日：2009-02-19

申请人： Maiko Tanabe ,  Chihiro Uematsu ,  Hirokazu Nishida ,  Kenko Uchida

发明人： Maiko Tanabe ,  Chihiro Uematsu ,  Hirokazu Nishida ,  Kenko Uchida

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  C12Q2521/501 ,  C12Q2565/301

摘要： It is intended to provide an assay for the presence, absence or amount of a nucleic-acid fragment having a certain nucleotide sequence, for example, a polyA length, a difference in the number of repetition of a direct repeat sequence (e.g., microsatellite), single nucleotide substitution (or single nucleotide polymorphism), and nucleotide sequence insertion or deletion, and to provide a genetic testing using the same. The present invention relates to a nucleotide analysis method, comprising: hybridizing at least two probes to a nucleic-acid fragment; ligating the at least two probes using ligase; exchanging, to ATP, pyrophosphoric acid produced through the ligation reaction; and detecting chemiluminescence reaction dependent on the ATP.

摘要翻译： 旨在提供具有某一核苷酸序列的核酸片段的存在，不存在或量的测定，例如polyA长度，直接重复序列（例如，微卫星）的重复次数的差异， ，单核苷酸取代（或单核苷酸多态性）和核苷酸序列插入或缺失，并提供使用其的遗传检测。 本发明涉及一种核苷酸分析方法，包括：将至少两个探针与核酸片段杂交; 使用连接酶连接至少两个探针; 通过连接反应产生的ATP，焦磷酸交换; 并根据ATP检测化学发光反应。

公开(公告)号：US20090189063A1

公开(公告)日：2009-07-30

申请号：US12330374

申请日：2008-12-08

申请人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

发明人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

IPC分类号： H01J49/00

CPC分类号： G01N33/6851 ,  G01N33/6848 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/004

摘要： During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. The information contained in the MSn spectrum is effectively utilized in each step of the MSn involving a multi-stage dissociation and mass spectroscopy (MSn), so that the flows for the determination of the next analysis content and the selection of the parent ion for the MSn+1 analysis, for example, can be optimized within the real time of measurement and with high efficiency and accuracy. Thus, a target of concern to the user can be subjected to tandem mass spectroscopy without wasteful measurement.

摘要翻译： 在通过串联质谱法对蛋白质或肽进行结构分析期间，作为串联质谱目标，可以避免衍生自已经测量并且大量表达的蛋白质的肽离子。 迄今为止难以分析的，从微量蛋白质衍生的肽可以在实时测定中作为串联质谱图目标自动确定。 关于已经测量的蛋白质和源自蛋白质的肽的数据被自动存储在内部数据库中。 存储的数据与测量数据高精度对照以确定同位素峰。 以这种方式，可以在测量的实际时间内进行未被测定为下一个串联分析的靶标的肽峰的过程，并且可以避免衍生自相同蛋白质的肽的冗余测量。 包含在MSn谱中的信息在涉及多级解离和质谱（MSn）的MSn的每个步骤中被有效地利用，使得用于确定下一个分析内容的流程和为母体离子的选择 例如，MSn + 1分析可以在实时测量和高效率和精确度下进行优化。 因此，用户关注的目标可以进行串联质谱，无需浪费测量。

公开(公告)号：US20070292897A1

公开(公告)日：2007-12-20

申请号：US11812156

申请日：2007-06-15

申请人： Yoshiaki Yazawa ,  Takashi Anazawa ,  Kenko Uchida

发明人： Yoshiaki Yazawa ,  Takashi Anazawa ,  Kenko Uchida

IPC分类号： G01N33/542 ,  G01N33/53 ,  C12M3/00

CPC分类号： G01N33/558 ,  G01N33/54386

摘要： There is provided a simple, inexpensive reaction analysis kit and system capable of performing highly sensitive, quantitative measurement. A chemical luminescence reaction is employed and a sensor element is used to detect the reaction in a highly sensitive manner. That is, a reaction detection plate is used to transmit a signal detected in the sensor element via a reader coil and a reader and then analyze the reaction. The reaction detection plate has a) a membrane, b) a first antibody impregnated section that is disposed such that it faces the membrane and holds a first labeled antibody that specifically binds to a substance to be analyzed, c) a second antibody immobilized section that is provided in part of the membrane and has an immobilized second antibody, the second antibody specifically binding to the substance to be analyzed, and d) a sensor element that is disposed such that it faces the second antibody immobilized section and includes a light detector and a signal transceiver.

摘要翻译： 提供了一种简单，便宜的反应分析试剂盒和系统，能够进行高灵敏度，定量测量。 采用化学发光反应，使用传感器元件以高度敏感的方式检测反应。 也就是说，反应检测板用于通过读取器线圈和读取器传输在传感器元件中检测到的信号，然后分析反应。 反应检测板具有a）膜，b）第一抗体浸渍部分，其被设置为使其面向膜并保持特异性结合待分析物质的第一标记抗体，c）第二抗体固定化部分，其中 提供在膜的一部分中，并且具有固定的第二抗体，特异性结合待分析物质的第二抗体，和d）设置为使得其面对第二抗体固定化部分并且包括光检测器的传感器元件， 一个信号收发器。

公开(公告)号：US06756014B2

公开(公告)日：2004-06-29

申请号：US10022735

申请日：2001-12-20

申请人： Michael Himmelhaus ,  Kenko Uchida ,  Hiroyuki Takei

发明人： Michael Himmelhaus ,  Kenko Uchida ,  Hiroyuki Takei

IPC分类号： G01N3348

CPC分类号： G01N33/54313 ,  Y10T436/25 ,  Y10T436/255

摘要： The invention provides a biochemical sensor with probes uniformly caught in each section. The probes used for detecting a substance of interest are caught in advance on particles, and the particles are fixed in each of sections arranged in form of lattice using a chemical patterning method on the surface of a baseplate. In each section, the particles attached with probes caught on the surface are fixed in single layer and tightly packed. The quantity of the particles fixed on the baseplate is determined by using a light scattering from the particles or by labeling the particles in advance with fluorescent substance. Therefore, the number of probes caught in each section of individual biochemical sensor is determined so as to allow the substance of interest to be detected with high accuracy.

摘要翻译： 本发明提供一种生物化学传感器，其中每个部分均匀地夹有探针。 用于检测感兴趣物质的探针被预先捕获在颗粒上，并且在基板的表面上使用化学图案化方法将颗粒固定在格子形式的每个部分中。 在每个部分中，附着在表面上的探针附着的颗粒固定在单层中并紧密包装。 通过使用来自颗粒的光散射或通过用荧光物质预先标记颗粒来确定固定在基板上的颗粒的量。 因此，确定各个生物化学传感器的每个部分中捕获的探针的数量，以便能高精度地检测感兴趣的物质。

公开(公告)号：US06216538B1

公开(公告)日：2001-04-17

申请号：US08742695

申请日：1996-11-04

申请人： Kenji Yasuda ,  Shin-ichiro Umemura ,  Kenichi Kawabata ,  Kazuo Takeda ,  Kenko Uchida ,  Yoshinori Harada ,  Masao Kamahori ,  Kazuaki Sasaki

发明人： Kenji Yasuda ,  Shin-ichiro Umemura ,  Kenichi Kawabata ,  Kazuo Takeda ,  Kenko Uchida ,  Yoshinori Harada ,  Masao Kamahori ,  Kazuaki Sasaki

IPC分类号： G01H1700

CPC分类号： B01D21/283 ,  G10K15/00

摘要： An ultrasonic manipulation apparatus has a plurality of ultrasonic wave oscillators arranged in two dimensions to trap, fix or move particles to an optional position in the solution or perform cell fusion by using a gradient force obtained by superposing one over another the gradient force fields generated by ultrasonic waves produced by a plurality of ultrasonic wave oscillators. The ultrasonic wave oscillators, functioning independently of one another, can emit ultrasonic waves with optional intensities and phases, and by using an external force produced by superposed gradient force fields generated by ultrasonic waves, particles are handled easily.

摘要翻译： 超声波操作装置具有多个超声波振荡器，其被设置成二维排列，以将颗粒捕获，固定或移动到溶液中的可选位置，或者通过使用通过将颗粒叠加在另一个上而产生的梯度力场而获得的梯度力进行细胞融合 由多个超声波振荡器产生的超声波。 彼此独立起作用的超声波振荡器可以发射具有可选强度和相位的超声波，并且通过使用由超声波产生的叠加梯度力场产生的外力，可以容易地处理颗粒。

公开(公告)号：US07985384B2

公开(公告)日：2011-07-26

申请号：US11812156

申请日：2007-06-15

申请人： Yoshiaki Yazawa ,  Takashi Anazawa ,  Kenko Uchida

发明人： Yoshiaki Yazawa ,  Takashi Anazawa ,  Kenko Uchida

IPC分类号： G01N21/75 ,  G01N31/22 ,  G01N33/52

CPC分类号： G01N33/558 ,  G01N33/54386

摘要： There is provided a simple, inexpensive reaction analysis kit and system capable of performing highly sensitive, quantitative measurement. A chemical luminescence reaction is employed and a sensor element is used to detect the reaction in a highly sensitive manner. That is, a reaction detection plate is used to transmit a signal detected in the sensor element via a reader coil and a reader and then analyze the reaction. The reaction detection plate has a) a membrane, b) a first antibody impregnated section that is disposed such that it faces the membrane and holds a first labeled antibody that specifically binds to a substance to be analyzed, c) a second antibody immobilized section that is provided in part of the membrane and has an immobilized second antibody, the second antibody specifically binding to the substance to be analyzed, and d) a sensor element that is disposed such that it faces the second antibody immobilized section and includes a light detector and a signal transceiver.

摘要翻译： 提供了一种简单，便宜的反应分析试剂盒和系统，能够进行高灵敏度，定量测量。 采用化学发光反应，使用传感器元件以高度敏感的方式检测反应。 也就是说，反应检测板用于通过读取器线圈和读取器传输在传感器元件中检测到的信号，然后分析反应。 反应检测板具有a）膜，b）第一抗体浸渍部分，其被设置为使其面向膜并保持特异性结合待分析物质的第一标记抗体，c）第二抗体固定化部分，其中 提供在膜的一部分中并且具有固定的第二抗体，特异性结合待分析物质的第二抗体，以及d）设置成使得其面向第二抗体固定化部分并且包括光检测器的传感器元件， 一个信号收发器。

公开(公告)号：US20090069663A1

公开(公告)日：2009-03-12

申请号：US12267056

申请日：2008-11-07

申请人： Akihiko Kandori ,  Tsuyoshi Miyashita ,  Keiji Tsukada ,  Kenko Uchida ,  Hideo Kawaguchi ,  Minoru Sakairi

发明人： Akihiko Kandori ,  Tsuyoshi Miyashita ,  Keiji Tsukada ,  Kenko Uchida ,  Hideo Kawaguchi ,  Minoru Sakairi

IPC分类号： A61B5/05

CPC分类号： A61B5/1101 ,  A61B5/4082 ,  A61B5/6824 ,  A61B5/6826 ,  A61B5/6828 ,  A61B5/6838 ,  A61B5/7239

摘要： A living body inspection apparatus including an oscillation coil which passes an AC current, a detection coil which detects an AC magnetic field generated from the detection coil, an amplification circuit which amplifies a voltage generated by the magnetic field induced by the detection coil, a detecting unit for detecting the output signal of the amplification circuit, a low pass filter to which the output signal of the detecting unit is input, a unit for setting the oscillation coil and detection coil in first and second regions of the living body, a recording unit for recording the output of the low pass filter while the first region and the second region of the living body are moving and a displaying unit for displaying the data recorded in the recording unit or results of analysis of the recorded data.

摘要翻译： 一种生物体检查装置，包括通过交流电流的振荡线圈，检测由检测线圈产生的交流磁场的检测线圈，放大电路，放大电路，放大由检测线圈感应出的磁场产生的电压， 用于检测放大电路的输出信号的单元，输入检测单元的输出信号的低通滤波器，用于在生物体的第一和第二区域设置振荡线圈和检测线圈的单元，记录单元 用于在活体的第一区域和第二区域移动时记录低通滤波器的输出，以及用于显示记录在记录单元中的数据的显示单元或记录数据的分析结果。