公开(公告)号：US20060257953A1

公开(公告)日：2006-11-16

申请号：US11410227

申请日：2006-04-24

申请人： Shohei Koide

发明人： Shohei Koide

IPC分类号： G01N33/574

CPC分类号： C07K14/78 ,  C07K16/00 ,  C07K16/18 ,  C07K16/40 ,  C07K2317/565 ,  C07K2318/20 ,  C12N15/1037 ,  C12N15/1044 ,  C40B40/02 ,  G01N33/68 ,  G01N33/6845 ,  G01N33/78 ,  G01N2333/78

摘要： A fibronectin type III (Fn3) polypeptide monobody, a nucleic acid molecule encoding said monobody, and a variegated nucleic acid library encoding said monobody, are provided by the invention. Also provided are methods of preparing a Fn3 polypeptide monobody, and kits to perform said methods. Further provided is a method of identifying the amino acid sequence of a polypeptide molecule capable of binding to a specific binding partner (SBP) so as to form a polypeptide:SSP complex, and a method of identifying the amino acid sequence of a polypeptide molecule capable of catalyzing a chemical reaction with a catalyzed rate constant, kcat, and an uncatalyzed rate constant, kuncat, such that the ratio of kcat/kuncat is greater than 10.

摘要翻译： 本发明提供了纤维连接蛋白III型（Fn3）多肽单体，编码所述单体的核酸分子和编码所述单体的杂化核酸文库。 还提供了制备Fn3多肽单体的方法和用于实施所述方法的试剂盒。 还提供了鉴定能够结合特异性结合配偶体（SBP）以形成多肽SSP复合物的多肽分子的氨基酸序列的方法，以及鉴定能够分解多肽分子的氨基酸序列的方法 以催化速率常数k catcat催化化学反应，以及未催化速率常数kcat，使得k cat / SUB的比例 > / k 大于10。

公开(公告)号：US20060114280A1

公开(公告)日：2006-06-01

申请号：US11288359

申请日：2005-11-29

申请人： Shohei Koide ,  Yuji Koga ,  Yusaku Watanabe

发明人： Shohei Koide ,  Yuji Koga ,  Yusaku Watanabe

IPC分类号： B41J29/38

CPC分类号： B41J13/0036 ,  B41J13/106

摘要： When a print process is started, discharge speed of recording papers is first set to a first discharge speed set based on the case in which a recording paper receiving unit for placing recording papers is in a reference state. Then, when a record instruction is given for starting a process of recording images onto recording papers, it is determined whether or not the recording paper receiving unit is in the reference state. If it is determined that the recording paper receiving unit is in an extended state in which the recording paper receiving unit is extended from the reference state, the discharge speed of the recording papers is set to a second discharge speed that is faster than the first discharge speed. Then, following a paper feed operation and an image record operation, the recording papers are discharged according to the set discharge speed.

摘要翻译： 当打印处理开始时，记录纸的排出速度首先被设定为基于用于放置记录纸的记录纸接收单元处于基准状态的情况设定的第一排出速度。 然后，当记录指令用于启动将记录图像记录在记录纸上的处理时，确定记录纸接收单元是否处于参考状态。 如果确定记录纸接收单元处于其中记录纸接收单元从基准状态延伸的扩展状态，则记录纸的排出速度被设定为比第一次放电快的第二排出速度 速度。 然后，在进纸操作和图像记录操作之后，记录纸根据设定的排出速度排出。

公开(公告)号：US20140057807A1

公开(公告)日：2014-02-27

申请号：US13813409

申请日：2011-08-01

申请人： Andreas Loew ,  Brian Edward Vash ,  Shohei Koide ,  John Bernard Wojcik ,  Akiko Koide ,  Ryan Nicholas Gilbreth

发明人： Andreas Loew ,  Brian Edward Vash ,  Shohei Koide ,  John Bernard Wojcik ,  Akiko Koide ,  Ryan Nicholas Gilbreth

IPC分类号： C07K14/78

CPC分类号： C07K14/78 ,  C07K16/18 ,  C07K16/40 ,  C07K16/42 ,  C07K2317/34 ,  C07K2318/20

摘要： The here described invention discloses a combination of a top and bottom loop binder library using the CD and the FG loops of a number of FnIII domains (FnIII) (e.g., FnIII7, FnIII10 and FnIII14) together with the surface exposed residues of the beta-sheet. The invention also pertains to a method of forming a library of FnIII domain polypeptides useful in screening for the presence of one or more polypeptides having a selected binding or enzymatic activity.

摘要翻译： 本文所述的本发明公开了使用CD和多个FnIII结构域（FnIII）（例如FnIII7，FnIII10和FnIII14）的FG环的顶部和底部环结合物文库的组合以及β- 片。 本发明还涉及形成用于筛选具有选定结合或酶活性的一种或多种多肽的存在的FnIII结构域多肽文库的方法。

公开(公告)号：US20130267676A1

公开(公告)日：2013-10-10

申请号：US13565559

申请日：2012-08-02

申请人： Shohei Koide

发明人： Shohei Koide

IPC分类号： C07K14/78 ,  C12N15/10

CPC分类号： C07K14/78 ,  C07K16/00 ,  C07K2317/565 ,  C12N15/1037 ,  C12N15/1044 ,  C40B30/04 ,  G01N33/6845

摘要： The present invention provides a fibronectin type III (Fn3) molecule, wherein the Fn3 contains a stabilizing mutation. The present invention also provides Fn3 polypeptide monobodies, nucleic acid molecules encoding monobodies, and variegated nucleic acid libraries encoding such monobodies. Also provided are methods of preparing a Fn3 polypeptide monobody, and kits to perform the methods.

摘要翻译： 本发明提供了III型（Fn3）纤连蛋白，其中Fn3含有稳定突变。 本发明还提供Fn3多肽单体，编码单体的核酸分子，以及编码这种单体的杂化核酸文库。 还提供了制备Fn3多肽单体的方法和用于实施该方法的试剂盒。

公开(公告)号：US08263350B2

公开(公告)日：2012-09-11

申请号：US12826322

申请日：2010-06-29

申请人： Shohei Koide ,  Jin Huang ,  Akiko Koide

发明人： Shohei Koide ,  Jin Huang ,  Akiko Koide

IPC分类号： G01N33/53

CPC分类号： C07K7/06

摘要： The invention provides a molecular affinity clamp. The architecture of the affinity clamp is modular with two biorecognition modules, each capable of binding a target motif. The first biorecognition module has a recognition domain that possesses inherent or natural specificity for the target motif. The second biorecognition module also has a recognition domain that binds the motif. The two biorecognition modules are tethered together either directly, e.g., via a peptide bond between the two modules, or indirectly, e.g., via a linker moiety or linker. The invention further provides a novel affinity ligand which is specifically bound by the molecular affinity clamps of the invention.

摘要翻译： 本发明提供了一种分子亲和力钳。 亲和力夹具的结构是模块化的，具有两个生物识别模块，每个模块能够结合靶基序。 第一个生物识别模块具有对目标基序具有固有或自然特异性的识别结构域。 第二种生物识别模块还具有结合基序的识别结构域。 两个生物识别模块直接连接在一起，例如通过两个模块之间的肽键，或间接地例如通过连接体部分或连接体。 本发明还提供了通过本发明的分子亲和性夹具特异性结合的新的亲和配体。

公开(公告)号：US07858090B2

公开(公告)日：2010-12-28

申请号：US11409939

申请日：2006-04-24

申请人： Shohei Koide

发明人： Shohei Koide

IPC分类号： A61K39/395 ,  C07K16/22

CPC分类号： C07K14/78 ,  C07K16/00 ,  C07K16/18 ,  C07K16/40 ,  C07K2317/565 ,  C07K2318/20 ,  C12N15/1037 ,  C12N15/1044 ,  C40B40/02 ,  G01N33/68 ,  G01N33/6845 ,  G01N33/78 ,  G01N2333/78

摘要： A fibronectin type III (Fn3) polypeptide monobody, a nucleic acid molecule encoding said monobody, and a variegated nucleic acid library encoding said monobody, are provided by the invention. Also provided are methods of preparing a Fn3 polypeptide monobody, and kits to perform said methods. Further provided is a method of identifying the amino acid sequence of a polypeptide molecule capable of binding to a specific binding partner (SBP) so as to form a polypeptide:SSP complex, and a method of identifying the amino acid sequence of a polypeptide molecule capable of catalyzing a chemical reaction with a catalyzed rate constant, kcat, and an uncatalyzed rate constant, kuncat, such that the ratio of kcat/kuncat is greater than 10.

摘要翻译： 本发明提供了纤维连接蛋白III型（Fn3）多肽单体，编码所述单体的核酸分子和编码所述单体的杂化核酸文库。 还提供了制备Fn3多肽单体的方法和用于实施所述方法的试剂盒。 还提供了鉴定能够结合特异性结合配偶体（SBP）以形成多肽SSP复合物的多肽分子的氨基酸序列的方法，以及鉴定能够分解多肽分子的氨基酸序列的方法 以催化速率常数kcat和未催化速率常数kuncat催化化学反应，使得kcat / kuncat的比例大于10。

公开(公告)号：US20100099168A1

公开(公告)日：2010-04-22

申请号：US11981784

申请日：2007-10-31

申请人： Shohei Koide

发明人： Shohei Koide

IPC分类号： C07H21/04 ,  C12N15/63 ,  C12N1/21

CPC分类号： C07K14/78 ,  C07K16/00 ,  C07K16/18 ,  C07K16/40 ,  C07K2317/565 ,  C07K2318/20 ,  C12N15/1037 ,  C12N15/1044 ,  C40B40/02 ,  G01N33/68 ,  G01N33/6845 ,  G01N33/78 ,  G01N2333/78

摘要： A fibronectin type III (Fn3) polypeptide monobody, a nucleic acid molecule encoding said monobody, and a variegated nucleic acid library encoding said monobody, are provided by the invention. Also provided are methods of preparing a Fn3 polypeptide monobody, and kits to perform said methods. Further provided is a method of identifying the amino acid sequence of a polypeptide molecule capable of binding to a specific binding partner (SBP) so as to form a polypeptide:SSP complex, and a method of identifying the amino acid sequence of a polypeptide molecule capable of catalyzing a chemical reaction with a catalyzed rate constant, kcat, and an uncatalyzed rate constant, kuncat, such that the ratio of kcat/kuncat is greater than 10.

公开(公告)号：US20090326200A1

公开(公告)日：2009-12-31

申请号：US12313443

申请日：2008-11-19

申请人： Benjamin J. Luft ,  John J. Dunn ,  Shohei Koide ,  Catherine L. Lawson

发明人： Benjamin J. Luft ,  John J. Dunn ,  Shohei Koide ,  Catherine L. Lawson

IPC分类号： C07K14/195

CPC分类号： C07K14/20 ,  A61K39/00 ,  C07K2319/00 ,  Y02A50/401

摘要： Provided herein are OspA polypeptides from Lyme Disease-causing Borrelia having certain alteration(s). In one embodiment, the alteration(s) increase the conformational stability of the OspA polypeptide containing the alteration(s) while maintaining at least some of the antigenicity of the corresponding unaltered OspA polypeptide. In another embodiment, the altered OspA polypeptide has reduced cross-reactivity to hLFA-1, as compared to the corresponding unaltered OspA polypeptide.

摘要翻译： 本文提供了具有某些改变的来自莱姆病引起的疏螺旋体的OspA多肽。 在一个实施方案中，所述改变增加含有所述改变的OspA多肽的构象稳定性，同时保持相应未改变的OspA多肽的至少一些抗原性。 在另一个实施方案中，与相应的未改变的OspA多肽相比，改变的OspA多肽具有降低的与hLFA-1的交叉反应性。

公开(公告)号：US20090253899A1

公开(公告)日：2009-10-08

申请号：US11848135

申请日：2007-08-30

申请人： Shohei Koide

发明人： Shohei Koide

IPC分类号： C07K16/00

CPC分类号： C07K16/18 ,  C07K14/78 ,  C07K16/00 ,  C07K2317/565 ,  C07K2318/20

摘要： The present invention provides modified fibronectin type III (Fn3) molecules, and nucleic acid molecules encoding the modified Fn3 molecules. Also provided are methods of preparing these molecules, and kits to perform the methods.

摘要翻译： 本发明提供修饰的纤连蛋白III（Fn3）分子和编码修饰的Fn3分子的核酸分子。 还提供了制备这些分子的方法和用于执行方法的试剂盒。

公开(公告)号：US07588300B2

公开(公告)日：2009-09-15

申请号：US11236659

申请日：2005-09-28

申请人： Yuji Koga ,  Shohei Koide ,  Tetsuya Ouchi

发明人： Yuji Koga ,  Shohei Koide ,  Tetsuya Ouchi

IPC分类号： B41J29/38 ,  B41J19/00 ,  B41J11/42

CPC分类号： B41J11/0065

摘要： An image-forming apparatus is capable of performing a non-margin recording operation without a margin on a recording medium and providing a waiting time between a moment of termination of a movement of a carriage in one of opposite directions and a moment of initiation of a subsequent movement of the carriage in the other direction, if the non-margin recording operation is performed during the movement of the carriage in the above-indicated one direction. The carriage is moved in the other direction (returned) at a speed lower than that of the movement in the above-indicated one direction, so that the back surface of an edge portion (e.g., leading end portion) of the recording medium is protected from contamination with an ink mist produced from the ink droplets. The waiting time and the return speed of the carriage can be manually selected or changed by using an operator's control panel, so that the non-margin recording operation can be performed in a desired manner (in a high-image-quality mode or a high-speed mode).

摘要翻译： 图像形成装置能够在记录介质上执行无裕度的无边缘记录操作，并且在相反方向中的一个方向上的滑架的移动终止时刻和提供一个等待时间之间提供等待时间 如果在滑架在上述一个方向的移动期间执行非余量记录操作，则滑架在另一个方向上的后续移动。 滑架以比上述一个方向的运动速度低的方式在另一方向（返回）移动，使得记录介质的边缘部分（例如，前端部分）的后表面被保护 从由墨滴产生的墨雾的污染。 可以通过使用操作者的控制面板手动选择或更改托架的等待时间和返回速度，从而可以以期望的方式（以高图像质量模式或高的方式）执行非余量记录操作 速度模式）。