公开(公告)号：US20060143737A1

公开(公告)日：2006-06-29

申请号：US10611748

申请日：2003-06-30

申请人： Narendra Yadav

发明人： Narendra Yadav

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8218 ,  C12N15/8213

摘要： This invention relates to methods of controlling gene silencing using site-specific recombination. A variety of constructs are provided which are useful for conditional or regulated gene silencing in plants, comprising a suite of constitutive, inducible, tissue-specific or developmental stage-specific promoters operably linked to target sequences (TS). Recombinase inversion or excision yields double-stranded TS RNA, which thereby functions to trigger endogenous gene silencing mechanisms. By matching promoters, responsive to various inducers, plant tissues or plant developmental states with the recombinase systems, transcriptional stop fragments or introns and target sequences, gene silencing of virtually any target sequence may be modulated at any plant development stage or in any plant generation. This is especially useful, when genes responsible for gene silencing are down-regulated to permit expression of particular transgenes at levels greater than permitted when gene silencing is activated.

摘要翻译： 本发明涉及使用位点特异性重组来控制基因沉默的方法。 提供了可用于植物中条件或调节基因沉默的各种构建体，其包含与靶序列（TS）可操作地连接的组合的一组组成型，诱导型组织特异性或发育阶段特异性启动子。 重组酶反转或切除产生双链TS RNA，从而起到引发内源性基因沉默机制的作用。 通过匹配启动子，响应于各种诱导剂，植物组织或植物发育状态与重组酶系统，转录终止片段或内含子和靶序列，几乎任何靶序列的基因沉默可以在任何植物发育阶段或任何植物生成中调节。 当基因沉默的基因被下调以允许特异转基因在基因沉默被激活时大于许可的水平表达时，这是特别有用的。