公开(公告)号：US07195885B2

公开(公告)日：2007-03-27

申请号：US10639150

申请日：2003-08-12

申请人： Min Gao ,  Julie A. Lemm ,  Donald R. O'Boyle ,  Peter Nower ,  Karen Rigat ,  Jin-hua Sun

发明人： Min Gao ,  Julie A. Lemm ,  Donald R. O'Boyle ,  Peter Nower ,  Karen Rigat ,  Jin-hua Sun

IPC分类号： C12Q1/00 ,  C12Q1/70 ,  C12Q1/68 ,  G01N33/53 ,  G01N1/30

CPC分类号： C07D417/04 ,  A61K31/00 ,  A61K31/426 ,  A61K45/06 ,  C07D277/42 ,  C07D417/06 ,  C07D417/12 ,  C07D417/14 ,  C07D513/04 ,  G01N33/5767 ,  G01N2333/95 ,  G01N2500/10 ,  A61K2300/00

摘要： The present invention includes an assay useful for identifying inhibitors of Hepatitis C virus (HCV) activity. Particularly, the present invention is directed to a dual HCV assay useful for high throughput screening that quantifies both the amount of HCV RNA replication inhibitory activity associated with a test compound and the amount of cytotoxicity associated with that test compound. The present invention also includes compounds discovered using this assay, compositions containing such compounds and methods of treating Hepatitis C by the administration of such compounds. The present invention also includes reporter assays using enzymes associated with HCV RNA replication, as well as a cell line having ATTC Accession No. PTA-4583.

摘要翻译： 本发明包括可用于鉴定丙型肝炎病毒（HCV）活性抑制剂的测定法。 特别地，本发明涉及用于高通量筛选的双重HCV测定，其量化与测试化合物相关的HCV RNA复制抑制活性的量和与该测试化合物相关的细胞毒性的量。 本发明还包括使用该测定法发现的化合物，含有这些化合物的组合物和通过施用这些化合物治疗丙型肝炎的方法。 本发明还包括使用与HCV RNA复制相关的酶的报道分析，以及具有ATTC登录号PTA-4583的细胞系。