摘要:
The present invention relates to recombinant DNA which encodes the BsmAI restriction endonuclease as well as BsmAI methylase, expression of BsmAI restriction endonuclease and BsmAI methylase in E. coli cells containing the recombinant DNA, and purification of BsmAI endonuclease to near homogeneity.
摘要:
The present invention relates to a method for isolating and identifying the nucleotide sequence of the human gene for the type IV collagen &agr;5(IV) chain. The present invention is directed to the determination of the nucleotide sequence of the gene for the &agr;5(IV) collagen chain in individuals by any method known to the art, e.g. cloning from genomic DNA libraries or amplifying gene regions with the polymerase chain reaction (PCR) and studying their physical properties or nucleotide sequences. In addition, the invention is directed to the use of the nucleotide sequences of the &agr;5(IV) gene to amplify or identify the nucleotide sequences of the &agr;5(IV) gene.
摘要:
The present invention relates to recombinant DNA which encodes the BsmI restriction endonuclease as well as BsmI methyltransferases, expression of BsmI restriction endonuclease in E. coil cells containing the recombinant DNA by using a low copy number T7 expression vector pACYC-T7ter, and purification of BsmI restriction endonuclease by heat treatment and chromatography through heparin Sepharose column.
摘要:
The invention provides a primer group for detecting CpO island methylation of p16 gene using methylation specific fluorescence technique. The primer group comprises a pair of oligonucleotide primers and a fluorescence-labeled probe. Said oligonucleotide primer pair has base sequence represented by SEQ ID NO.1 and SEQ ID NO.2. Said fluorescence-labeled probe has base sequence represented by SEQ ID NO.3 or SEQ NO.4.
摘要翻译:本发明提供使用甲基化特异性荧光技术检测p16基因的CpO岛甲基化的引物组。 引物组包含一对寡核苷酸引物和荧光标记探针。 所述寡核苷酸引物对具有由SEQ ID NO.1和SEQ ID NO.2表示的碱基序列。 所述荧光标记探针具有由SEQ ID NO.3或SEQ ID NO.4表示的碱基序列。
摘要:
Embodiments of the invention relate to organizing data records in a relational database. An aspect of the invention includes creating index items for a plurality of data records. Each index item includes a counter and the creating results in a plurality of counters. The numerical values of counters in corresponding index items are updated for data records in the plurality of data records that are subjected to random access. The plurality of data records are reorganized based upon the numerical values of the plurality of counters.
摘要:
A method and apparatus to meter ink for electrographic printing is disclosed. An ink loading mechanism having an anilox roller fills ink from an ink supply into cells in the anilox roller having a plurality of valleys and lands that form the cells. The ink loading mechanism causes the valleys to be full or nearly full with the ink. The anilox roller rotates in a first direction. In one embodiment, a soft blade positioned slightly below surface of the lands removes ink from the cells and causes the valleys to be partially filled as the anilox roller rotates. A hard blade positioned at the surface of the lands to clean residue of ink on the surface of the lands as the anilox roller rotates. In another embodiment, a blanket roller rotationally engaged with the anilox roller pulls ink out of the cells and causes the valleys to be partially filled. The blanket roller rotates in a second direction. A first cleaning blade cleans tops of the lands of the cells.
摘要:
A linear surgical stapler includes an upper staple cartridge receiving half-section, a lower staple cartridge receiving half-section and a staple cartridge assembly. The staple cartridge assembly includes a cutting groove in which a reciprocating movement cutter is mounted. The cutter includes a bottom surface and a knife blade located at a far end of the cutter. The staple cartridge assembly includes a restricting member mounted therein and an elastic stopper. The elastic stopper abuts against the restricting member when the staple cartridge assembly is in an initial state. The restricting member breaks away from the staple cartridge assembly when the staple cartridge assembly is in a firing complete state, thereby preventing the cutter from sliding towards the far end of the staple cartridge. As a result, misfiring throughout the whole operation of the linear surgical stapler is prevented.
摘要:
The present invention provides an instrument for anorectal surgery, comprising a hollow main body, a suture junction disposed at a rear end of the main body and an inserting guider disposed at a front end of the main body. At an operating position, all or part of mucosa and tissues can get through the main body. At least one opening is made in the wall of the main body, and all or part of the mucosa and tissues can get into a hollow interior of the main body through the opening. Because of the openings on the wall of the main body, the present invention can be operated to perform the anorectal surgery of local and non-annular cutting, which facilitates a quick surgical operation, further allays the pain of the patients, and a better operation effect is obtained.
摘要:
A digital flexography system includes an imaging member with a charge generating layer formed from an array of addressable pixels and a charge transport layer thereon. Ink is delivered to the imaging member using a simple rough donor roll, rather than an anilox roll. Instead of controlling the amount of ink delivered using the anilox roll, the amount of ink is controlled by the pixels on the imaging member.
摘要:
In some aspects of the present application, a method of forming one or more layers of at least a portion of a drug delivery device (DDD) is described. The method can include providing a substrate; providing one or more DDD components that are dissolved or dispersed in one or more pharmaceutically compatible phase change inks; ejecting, by one or more nozzles, a first portion of the one or more pharmaceutically compatible phase change inks to form a first layer on the substrate; and ejecting, by the one or more nozzles, a second portion of the pharmaceutically compatible phase change inks to form a second layer over the first layer.