Method for cloning and expression of BsmAi restriction endonuclease and BsmAI methylase in E. coli
    21.
    发明授权
    Method for cloning and expression of BsmAi restriction endonuclease and BsmAI methylase in E. coli 失效
    用于在大肠杆菌中克隆和表达BsmAi限制性内切核酸酶和BsmAI甲基化酶的方法

    公开(公告)号:US06596524B2

    公开(公告)日:2003-07-22

    申请号:US09957005

    申请日:2001-09-20

    IPC分类号: C12N922

    CPC分类号: C12N9/22 C12N9/1007

    摘要: The present invention relates to recombinant DNA which encodes the BsmAI restriction endonuclease as well as BsmAI methylase, expression of BsmAI restriction endonuclease and BsmAI methylase in E. coli cells containing the recombinant DNA, and purification of BsmAI endonuclease to near homogeneity.

    摘要翻译: 本发明涉及编码BsmAI限制性内切核酸酶以及BsmAI甲基化酶的重组DNA,含有重组DNA的大肠杆菌细胞中BsmAI限制性内切核酸酶和BsmAI甲基化酶的表达,以及将BsmAI内切核酸酶纯化至接近同质性。

    Method for determining the nucleotide sequence of the gene for the &agr;5(IV) chain of human type IV collagen
    22.
    发明授权
    Method for determining the nucleotide sequence of the gene for the &agr;5(IV) chain of human type IV collagen 失效
    确定人IV型胶原的α5(IV)链的基因的核苷酸序列的方法

    公开(公告)号:US06576418B1

    公开(公告)日:2003-06-10

    申请号:US08692989

    申请日:1996-08-07

    IPC分类号: C12Q168

    摘要: The present invention relates to a method for isolating and identifying the nucleotide sequence of the human gene for the type IV collagen &agr;5(IV) chain. The present invention is directed to the determination of the nucleotide sequence of the gene for the &agr;5(IV) collagen chain in individuals by any method known to the art, e.g. cloning from genomic DNA libraries or amplifying gene regions with the polymerase chain reaction (PCR) and studying their physical properties or nucleotide sequences. In addition, the invention is directed to the use of the nucleotide sequences of the &agr;5(IV) gene to amplify or identify the nucleotide sequences of the &agr;5(IV) gene.

    摘要翻译: 本发明涉及用于分离和鉴定IV型胶原α5(IV)链的人基因的核苷酸序列的方法。 本发明涉及通过本领域已知的任何方法测定个体中α5(IV)胶原链的基因的核苷酸序列,例如, 通过聚合酶链反应(PCR)从基因组DNA文库克隆或扩增基因区域,并研究其物理性质或核苷酸序列。 此外,本发明涉及α5(IV)基因的核苷酸序列用于扩增或鉴定α5(IV)基因的核苷酸序列的用途。

    Method for cloning and producing the BsmI restriction endonuclease in E. coli
    23.
    发明授权
    Method for cloning and producing the BsmI restriction endonuclease in E. coli 失效
    用于在大肠杆菌中克隆和产生BsmI限制性内切核酸酶的方法

    公开(公告)号:US06335190B1

    公开(公告)日:2002-01-01

    申请号:US09693147

    申请日:2000-10-20

    IPC分类号: C12N1555

    CPC分类号: C12N9/22 C12N9/1007

    摘要: The present invention relates to recombinant DNA which encodes the BsmI restriction endonuclease as well as BsmI methyltransferases, expression of BsmI restriction endonuclease in E. coil cells containing the recombinant DNA by using a low copy number T7 expression vector pACYC-T7ter, and purification of BsmI restriction endonuclease by heat treatment and chromatography through heparin Sepharose column.

    摘要翻译: 本发明涉及编码BsmI限制性内切核酸酶以及BsmI甲基转移酶的重组DNA,通过使用低拷贝数T7表达载体pACYC-T7ter,含有重组DNA的大肠杆菌细胞中BsmI限制性内切核酸酶的表达,以及纯化BsmI 通过热处理和通过肝素琼脂糖凝胶柱色谱法进行限制性内切核酸酶。

    PRIMER GROUP FOR DETECTING CPG ISLAND METHYLATION OF P16 GENE USING METHYLATION- SPECIFIC FLUORESCENCE TECHNIQUE
    24.
    发明申请
    PRIMER GROUP FOR DETECTING CPG ISLAND METHYLATION OF P16 GENE USING METHYLATION- SPECIFIC FLUORESCENCE TECHNIQUE 有权
    使用甲基特异性荧光技术检测P16基因的CPG岛状甲基化的前体组

    公开(公告)号:US20150218642A1

    公开(公告)日:2015-08-06

    申请号:US13978956

    申请日:2011-01-01

    申请人: Dajun Deng Jing Zhou

    发明人: Dajun Deng Jing Zhou

    IPC分类号: C12Q1/68

    摘要: The invention provides a primer group for detecting CpO island methylation of p16 gene using methylation specific fluorescence technique. The primer group comprises a pair of oligonucleotide primers and a fluorescence-labeled probe. Said oligonucleotide primer pair has base sequence represented by SEQ ID NO.1 and SEQ ID NO.2. Said fluorescence-labeled probe has base sequence represented by SEQ ID NO.3 or SEQ NO.4.

    摘要翻译: 本发明提供使用甲基化特异性荧光技术检测p16基因的CpO岛甲基化的引物组。 引物组包含一对寡核苷酸引物和荧光标记探针。 所述寡核苷酸引物对具有由SEQ ID NO.1和SEQ ID NO.2表示的碱基序列。 所述荧光标记探针具有由SEQ ID NO.3或SEQ ID NO.4表示的碱基序列。

    Method of and apparatus for organizing data records in a relational database
    25.
    发明授权
    Method of and apparatus for organizing data records in a relational database 有权
    在关系数据库中组织数据记录的方法和装置

    公开(公告)号:US08972403B2

    公开(公告)日:2015-03-03

    申请号:US13365394

    申请日:2012-02-03

    IPC分类号: G06F17/30 G06F7/00

    CPC分类号: G06F17/30312

    摘要: Embodiments of the invention relate to organizing data records in a relational database. An aspect of the invention includes creating index items for a plurality of data records. Each index item includes a counter and the creating results in a plurality of counters. The numerical values of counters in corresponding index items are updated for data records in the plurality of data records that are subjected to random access. The plurality of data records are reorganized based upon the numerical values of the plurality of counters.

    摘要翻译: 本发明的实施例涉及在关系数据库中组织数据记录。 本发明的一个方面包括创建多个数据记录的索引项。 每个索引项目包括计数器,并且在多个计数器中创建结果。 针对经过随机存取的多个数据记录中的数据记录,对相应索引项中的计数器的数值进行更新。 基于多个计数器的数值重新组织多个数据记录。

    Anilox metering system for electrographic printing
    26.
    发明授权
    Anilox metering system for electrographic printing 有权
    用于电印的网纹计量系统

    公开(公告)号:US08820233B2

    公开(公告)日:2014-09-02

    申请号:US12566568

    申请日:2009-09-24

    CPC分类号: G03G15/104

    摘要: A method and apparatus to meter ink for electrographic printing is disclosed. An ink loading mechanism having an anilox roller fills ink from an ink supply into cells in the anilox roller having a plurality of valleys and lands that form the cells. The ink loading mechanism causes the valleys to be full or nearly full with the ink. The anilox roller rotates in a first direction. In one embodiment, a soft blade positioned slightly below surface of the lands removes ink from the cells and causes the valleys to be partially filled as the anilox roller rotates. A hard blade positioned at the surface of the lands to clean residue of ink on the surface of the lands as the anilox roller rotates. In another embodiment, a blanket roller rotationally engaged with the anilox roller pulls ink out of the cells and causes the valleys to be partially filled. The blanket roller rotates in a second direction. A first cleaning blade cleans tops of the lands of the cells.

    摘要翻译: 公开了一种用于电印印刷的墨水计量方法和装置。 具有网纹辊的墨水装载机构将油墨从墨水供给装入具有形成该单元的多个谷和平台的网纹辊中的单元中。 墨水装载机构使墨水充满或接近满。 网纹辊沿第一方向旋转。 在一个实施例中,稍微位于焊盘表面下方的软刀片从电池中去除墨水,并且当网纹辊旋转时使谷部分被填充。 位于平台表面的硬刀片,以在网纹辊旋转时清洁焊盘表面上的油墨残留物。 在另一个实施例中,与网纹辊旋转接合的橡皮布辊将墨水从电池中拉出并导致谷部分地被填充。 橡皮布滚筒沿第二个方向旋转。 第一清洁刀片清洁细胞的脊部的顶部。

    STAPLE CHAMBER ASSEMBLY AND LINEAR SURGICAL STITCHING DEVICE USING SAID STAPLE CHAMBER ASSEMBLY
    27.
    发明申请
    STAPLE CHAMBER ASSEMBLY AND LINEAR SURGICAL STITCHING DEVICE USING SAID STAPLE CHAMBER ASSEMBLY 有权
    书架式组装和线性外科手术装置

    公开(公告)号:US20130327808A1

    公开(公告)日:2013-12-12

    申请号:US13993070

    申请日:2011-12-05

    IPC分类号: A61B17/072

    摘要: A linear surgical stapler includes an upper staple cartridge receiving half-section, a lower staple cartridge receiving half-section and a staple cartridge assembly. The staple cartridge assembly includes a cutting groove in which a reciprocating movement cutter is mounted. The cutter includes a bottom surface and a knife blade located at a far end of the cutter. The staple cartridge assembly includes a restricting member mounted therein and an elastic stopper. The elastic stopper abuts against the restricting member when the staple cartridge assembly is in an initial state. The restricting member breaks away from the staple cartridge assembly when the staple cartridge assembly is in a firing complete state, thereby preventing the cutter from sliding towards the far end of the staple cartridge. As a result, misfiring throughout the whole operation of the linear surgical stapler is prevented.

    摘要翻译: 线性外科缝合器包括接收半部分的上部钉仓,下部钉仓接收半部分和钉仓组件。 钉仓组件包括其中安装有往复运动切割器的切割槽。 切割器包括位于切割器远端的底面和刀片。 钉仓组件包括安装在其中的限制构件和弹性塞子。 当钉仓组件处于初始状态时,弹性止动件抵靠限制构件。 当钉仓组件处于点火完成状态时,限制构件脱离钉仓组件,从而防止刀具朝向钉仓的远端滑动。 结果,可以防止线性外科缝合器整个操作过程中的点火不足。

    Instrument for anorectal surgery
    28.
    发明授权
    Instrument for anorectal surgery 有权
    肛门直肠手术器械

    公开(公告)号:US08585719B2

    公开(公告)日:2013-11-19

    申请号:US12810693

    申请日:2008-12-25

    IPC分类号: A61B17/04

    摘要: The present invention provides an instrument for anorectal surgery, comprising a hollow main body, a suture junction disposed at a rear end of the main body and an inserting guider disposed at a front end of the main body. At an operating position, all or part of mucosa and tissues can get through the main body. At least one opening is made in the wall of the main body, and all or part of the mucosa and tissues can get into a hollow interior of the main body through the opening. Because of the openings on the wall of the main body, the present invention can be operated to perform the anorectal surgery of local and non-annular cutting, which facilitates a quick surgical operation, further allays the pain of the patients, and a better operation effect is obtained.

    摘要翻译: 本发明提供了一种肛门直肠手术器械,包括中空主体,设置在主体后端的缝线接头和设置在主体前端的插入引导件。 在一个操作的位置,全部或部分粘膜和组织可以穿过主体。 在主体的壁上形成至少一个开口,并且全部或部分粘膜和组织可以通过开口进入主体的中空内部。 由于主体壁上的开口,本发明可以被操作以执行局部和非环形切割的肛门直肠手术,这有助于快速的外科手术,进一步使患者的疼痛和更好的操作 效果得到。

    APPARATUS FOR DIGITAL FLEXOGRAPHIC PRINTING
    29.
    发明申请
    APPARATUS FOR DIGITAL FLEXOGRAPHIC PRINTING 有权
    数码复印机的设备

    公开(公告)号:US20130092038A1

    公开(公告)日:2013-04-18

    申请号:US13274659

    申请日:2011-10-17

    IPC分类号: B41F9/00

    CPC分类号: B41M5/20 B41M1/04

    摘要: A digital flexography system includes an imaging member with a charge generating layer formed from an array of addressable pixels and a charge transport layer thereon. Ink is delivered to the imaging member using a simple rough donor roll, rather than an anilox roll. Instead of controlling the amount of ink delivered using the anilox roll, the amount of ink is controlled by the pixels on the imaging member.

    摘要翻译: 数字柔版印刷系统包括具有由可寻址像素阵列和其上的电荷传输层形成的电荷产生层的成像构件。 使用简单的粗制供体辊而不是网纹辊将油墨输送到成像构件。 代替控制使用网纹辊输送的墨水量,墨水的量由成像构件上的像素控制。

    DIRECT INKJET FABRICATION OF DRUG DELIVERY DEVICES
    30.
    发明申请
    DIRECT INKJET FABRICATION OF DRUG DELIVERY DEVICES 有权
    药物递送装置的直接喷嘴制造

    公开(公告)号:US20120315333A1

    公开(公告)日:2012-12-13

    申请号:US13156503

    申请日:2011-06-09

    申请人: Jing Zhou Shu Chang

    发明人: Jing Zhou Shu Chang

    IPC分类号: A61K9/00 B05C11/00

    摘要: In some aspects of the present application, a method of forming one or more layers of at least a portion of a drug delivery device (DDD) is described. The method can include providing a substrate; providing one or more DDD components that are dissolved or dispersed in one or more pharmaceutically compatible phase change inks; ejecting, by one or more nozzles, a first portion of the one or more pharmaceutically compatible phase change inks to form a first layer on the substrate; and ejecting, by the one or more nozzles, a second portion of the pharmaceutically compatible phase change inks to form a second layer over the first layer.

    摘要翻译: 在本申请的一些方面,描述了形成药物递送装置(DDD)的至少一部分的一个或多个层的方法。 该方法可以包括提供基底; 提供溶解或分散在一种或多种药学上相容的相变油墨中的一种或多种DDD组分; 通过一个或多个喷嘴喷射所述一种或多种药学上相容的相变油墨的第一部分以在所述基底上形成第一层; 以及通过所述一个或多个喷嘴喷射所述药学上相容的相变油墨的第二部分,以在所述第一层上形成第二层。