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公开(公告)号:US5767379A
公开(公告)日:1998-06-16
申请号:US554586
申请日:1995-11-06
申请人: Chris Baszczynski , Elizabeth Hood , Sheila Maddock , Terry EuClaire Meyer , James C. Register, III , Derrick Witcher , John A. Howard
发明人: Chris Baszczynski , Elizabeth Hood , Sheila Maddock , Terry EuClaire Meyer , James C. Register, III , Derrick Witcher , John A. Howard
CPC分类号: C07K14/465 , C07K14/46 , C12N15/8257
摘要: A method for commercial production of avidin entails heterologous expression of the protein in plants, in native conformation, at an expression level such that avidin represents at least 0.1% of total extracted protein. A genetic map of the integration locus allows for the identification of the avidin-expressing plant. Genetic loci on a plant chromosome are revealed that support high levels of avidin expression and that can be used as a site of integration for high level expression of other genes of interest.
摘要翻译: 用于商业生产抗生物素蛋白的方法需要以天然构象在植物中异源表达蛋白质,其表达水平使得抗生物素蛋白占总提取蛋白质的至少0.1%。 整合位点的遗传图可以鉴定表达抗生物素蛋白的植物。 显示植物染色体上的遗传基因座,其支持高水平的抗生物素蛋白表达,并且可以用作其它感兴趣的基因的高水平表达的整合位点。