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公开(公告)号：US06429358B1
公开(公告)日：2002-08-06
申请号：US09403618
申请日：1999-11-05
申请人： Karen E. Broglie
发明人： Karen E. Broglie
IPC分类号： C12N1582
CPC分类号： C12N9/2457 , C12N15/8245 , C12Y302/01041
摘要： This invention relates to isolated nucleic acid fragments encoding all or a substantial portion of a corn pullulanase. The invention also relates to the construction of chimeric genes encoding all or a portion of a corn pullulanase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of corn pullulanase in a transformed host cell.
摘要翻译： 本发明涉及编码玉米支链淀粉酶的全部或大部分的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码玉米支链淀粉酶的全部或部分的嵌合基因，其中嵌合基因的表达导致转化的宿主细胞中玉米支链淀粉酶水平的变化。
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公开(公告)号：US20150305341A1
公开(公告)日：2015-10-29
申请号：US14793893
申请日：2015-07-08
IPC分类号： A01N57/16 , A01N65/20 , A01N65/44 , C12N15/82 , C12N15/113
CPC分类号： A01N57/16 , A01N63/00 , A01N65/20 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N15/8286 , C12N2310/11 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barberi, D. speciosa, or D. undecimpunctata howardi. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译： 提供了使用沉积元件的方法和组合物，当由有害生物摄取的诸如鞘翅目植物病虫害（Diaprotica plant pest）的害虫时，会降低害虫中靶序列的表达。 在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。 本发明提供了SEQ ID NO：1-236中任一项所述的各种靶多核苷酸或其活性变体及其片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性 活动）。 进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。 在具体实施方案中，害虫是D. virgifera virgifera，D.barberi，D. speciosa或D. undecimpunctata howardi。 还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。
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公开(公告)号：US20110054007A1
公开(公告)日：2011-03-03
申请号：US12868994
申请日：2010-08-26
CPC分类号： A01N57/16 , A01N63/00 , A01N65/20 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N15/8286 , C12N2310/11 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barberi, D. speciosa, or D. undecimpunctata howardi. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译： 提供了使用沉积元件的方法和组合物，当由有害生物摄取的诸如鞘翅目植物病虫害（Diaprotica plant pest）的害虫时，会降低害虫中靶序列的表达。 在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。 本发明提供了SEQ ID NO：1-236中任一项所述的各种靶多核苷酸或其活性变体及其片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性 活动）。 进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。 在具体实施方案中，害虫是D. virgifera virgifera，D.barberi，D. speciosa或D. undecimpunctata howardi。 还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。
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公开(公告)号：US07754942B2
公开(公告)日：2010-07-13
申请号：US11777415
申请日：2007-07-13
CPC分类号： C08B30/00 , C12N15/8245
摘要： The present invention is directed to a starch comprising a novel percentage of actual amylose. A grain comprising the starch is also embodied by the present invention. The present invention is also directed to a plant comprising the novel starch.
摘要翻译： 本发明涉及包含实际直链淀粉的新百分比的淀粉。 包含淀粉的颗粒也由本发明体现。 本发明还涉及包含新型淀粉的植物。
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