公开(公告)号：US20120029831A1

公开(公告)日：2012-02-02

申请号：US13145763

申请日：2010-01-15

申请人： Kazuteru Hoshishima ,  Shigeyuki Nakada

发明人： Kazuteru Hoshishima ,  Shigeyuki Nakada

IPC分类号： G01N21/64 ,  G06F19/00

CPC分类号： G01N21/6408 ,  G01N15/147 ,  G01N21/6428 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441

摘要： A fluorescence detecting device receives fluorescence emitted by n kinds of measurement objects within wavelength bands FLk (k is an integer of 1 to n) set so that the fluorescence intensity of fluorescence emitted by a measurement object k becomes higher than that of fluorescence emitted by the other one or more measurement objects, and acquires fluorescent signals corresponding to the wavelength bands FLk (k is an integer of 1 to n). Each of the fluorescent signals is subjected to frequency-down conversion by mixing it with a modulation signal for modulating the intensity of a laser beam Lk (k=1) corresponding to at least one of the wavelength bands FLk to produce fluorescence data including the phase delay and intensity amplitude of the fluorescent signal. The fluorescence data is corrected to calculate the phase delay and a fluorescence relaxation time is calculated using the phase delay.

摘要翻译： 荧光检测装置接收被设定的波长范围FLk（k为1〜n的整数）内的n种测量对象发出的荧光，使得测量对象k发射的荧光的荧光强度比由 其他一个或多个测量对象，并且获取对应于波长带FLk（k为1至n的整数）的荧光信号。 通过将每个荧光信号与调制信号进行降频转换，该调制信号用于调制与至少一个波长带FLk相对应的激光束Lk（k = 1）的强度，以产生包括相位的荧光数据 荧光信号的延迟和强度幅度。 校正荧光数据以计算相位延迟，并使用相位延迟来计算荧光弛豫时间。