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公开(公告)号：US06190898B1
公开(公告)日：2001-02-20
申请号：US08609362
申请日：1996-03-01
IPC分类号： C12N914
CPC分类号： C12N9/2437 , C12Y302/01004
摘要： A method for preparing a crystalline cellulase enzyme is provided which comprises preparing an aqueous solution containing cellulase enzyme and adding to the aqueous solution a salt comprising an anion selected from the group consisting of sulfate, phosphate, formate, acetate, sorbate, chloride, bromide, fluoride or iodide, and a cation selected from the group consisting of sodium, ammonium, magnesium, potassium or calcium, wherein the aqueous solution is at a temperature between 10° C. and 60° C.
摘要翻译： 提供了一种制备结晶纤维素酶的方法，其包括制备含有纤维素酶的水溶液，并向该水溶液中加入包含选自硫酸盐，磷酸盐，甲酸盐，乙酸盐，山梨酸盐，氯化物，溴化物， 氟化物或碘化物，以及选自钠，铵，镁，钾或钙的阳离子，其中所述水溶液的温度为10℃至60℃。
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公开(公告)号：US06105786A
公开(公告)日：2000-08-22
申请号：US887494
申请日：1997-07-02
CPC分类号： C11D3/046 , C11D11/0082 , C11D3/386 , C12N9/00 , C12N9/54 , C12P1/00 , Y10S435/816
摘要： A process is disclosed for preparing a detergent powder containing enzymes where the enzymes are extracted from a fermentation broth with a salt and surfactant mixture and directly agglomerated with detergent paste and dried to form the detergent powder. The process results in a two phase system where the enzyme is extracted into the surfactant rich phase and the second phase is salt rich. The process is especially useful for whole or clarified fermentation broths.
摘要翻译： 公开了一种制备含有酶的洗涤剂粉末的方法，其中用盐和表面活性剂混合物从发酵液中提取酶，并用洗涤剂糊剂直接附聚并干燥以形成洗涤剂粉末。 该过程产生两相体系，其中酶被萃取到富表面活性剂相中，第二相是富含盐的。 该方法对于整个或澄清的发酵液特别有用。
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公开(公告)号：US20140057015A1
公开(公告)日：2014-02-27
申请号：US14000458
申请日：2012-02-29
申请人： Nathaniel T. Becker
发明人： Nathaniel T. Becker
CPC分类号： C12N9/48 , A23K20/189 , A23K20/22 , A23K40/10 , A23K40/30 , C11D3/046 , C11D3/386 , C11D3/38672 , C11D17/06 , C12N9/16 , C12N9/52 , C12N9/98 , C12Y301/03 , C12Y304/21062
摘要： The present teachings provide ways of improving the distribution of high payload enzyme granules by reducing their size, and by mixing them with size-matched dummy particles containing sodium sulfate. The present teachings also provide methods of using the mixtures.
摘要翻译： 本教导提供了通过减小高有效载荷的酶颗粒的大小，并将其与尺寸匹配的含有硫酸钠的假颗粒混合来改进高有效载荷酶颗粒的分布的方法。 本教导还提供使用该混合物的方法。
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公开(公告)号：US20120309664A1
公开(公告)日：2012-12-06
申请号：US13491106
申请日：2012-06-07
申请人： Herbert B. Scher , Deborah S. Winetzky , Nathaniel T. Becker , Kathleen A. Clarkson , Mark S. Gebert , Troels N. Gravesen , Michael Partsuf
发明人： Herbert B. Scher , Deborah S. Winetzky , Nathaniel T. Becker , Kathleen A. Clarkson , Mark S. Gebert , Troels N. Gravesen , Michael Partsuf
CPC分类号： C11D3/38672 , C11D3/225 , C11D3/3707
摘要： The present invention generally relates to particulate compositions and methods for making the compositions. It specifically relates to improved spray drying methods that substantially reduce the production of small particles that pose industrial hygiene concerns for factory workers and product consumers. In a composition aspect, the present invention provides an atomized aqueous composition. The composition is formed using a spray-drying apparatus. It includes 0.001 to 0.10 weight percent of a polymer, which is selected from a group consisting of a cellulose-based polymer, a gum, and a synthetic polymer. It further includes at least one polypeptide, which is present in the aqueous composition at a concentration greater than 0.01 weight percent.
摘要翻译： 本发明一般涉及制备组合物的颗粒组合物和方法。 它具体涉及改进的喷雾干燥方法，其显着降低了造成工厂工人和产品消费者的工业卫生问题的小颗粒的生产。 在组合物方面，本发明提供雾化的水性组合物。 使用喷雾干燥装置形成组合物。 它包括0.001至0.10重量％的聚合物，其选自纤维素基聚合物，胶和合成聚合物。 它还包括至少一种多肽，其以大于0.01重量％的浓度存在于水性组合物中。
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公开(公告)号：US08309334B2
公开(公告)日：2012-11-13
申请号：US12342398
申请日：2008-12-23
CPC分类号： C11D3/38 , C11D3/222 , C11D3/3753 , C11D3/38672 , C11D17/0039
摘要： The present invention provides highly impact-resistant, water-soluble or water dispersible, low-dust granules comprising an active ingredient and methods for obtaining the same.
摘要翻译： 本发明提供包含活性成分的高抗冲击性，水溶性或水分散性低粉尘颗粒及其获得方法。
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公开(公告)号：US07566448B2
公开(公告)日：2009-07-28
申请号：US10626125
申请日：2003-07-23
IPC分类号： A61K38/48
CPC分类号： C11D3/3769 , A61K8/046 , A61K8/66 , A61K8/86 , A61Q11/00 , A61Q19/10 , C11D3/386 , C11D17/0043
摘要： The present invention relates to compositions comprising high molecular weight polymers, particularly polyethylene oxide polymers, wherein the high molecular weight polymer serves as an anti-misting agent to reduce the potential of aerosol generation from a composition when used in a desired environment. The invention further relates to methods of decreasing enzyme exposure from a personal care or cleaning product comprising a high molecular weight polymer.
摘要翻译： 本发明涉及包含高分子量聚合物，特别是聚环氧乙烷聚合物的组合物，其中当在期望的环境中使用时，高分子量聚合物用作防雾剂以降低组合物中气溶胶产生的潜力。 本发明还涉及减少包含高分子量聚合物的个人护理或清洁产品的酶暴露的方法。
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27.发明授权Preparation of a granule containing protein, corn starch and sugar layered on an inert particle 有权制备含有蛋白质，玉米淀粉和糖分散在惰性颗粒上的颗粒公开(公告)号：US07300779B2
公开(公告)日：2007-11-27
申请号：US10939576
申请日：2004-09-13
CPC分类号： C12N9/98 , Y10S530/812 , Y10S530/813
摘要： Granules that include a protein core are described. The protein core includes a protein matrix which includes a protein mixed together with a starch. The protein matrix can be layered over a seed particle or the protein core can be homogeneous. The protein can be an enzyme or a therapeutic protein.
摘要翻译： 描述了包含蛋白质核心的颗粒。 蛋白质核心包括蛋白质基质，其包括与淀粉混合在一起的蛋白质。 蛋白质基质可以分层在种子颗粒上，或蛋白质核心可以是均质的。 蛋白质可以是酶或治疗性蛋白质。
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公开(公告)号：US07018821B2
公开(公告)日：2006-03-28
申请号：US10176342
申请日：2002-06-20
CPC分类号： C11D3/38 , C11D3/222 , C11D3/3753 , C11D3/38672 , C11D17/0039
摘要： The present invention provides highly impact-resistant, water-soluble or water dispersible, low-dust granules comprising an active ingredient and methods for obtaining the same.
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29.发明授权Carbon dioxide soluble surfactant having two fluoroether CO2-philic tail groups and a head group 失效二氧化碳可溶性表面活性剂具有两个氟醚CO 2亲水性尾巴基团和头基公开(公告)号：US06638749B1
公开(公告)日：2003-10-28
申请号：US09004603
申请日：1998-01-08
申请人： Eric J. Beckman , Eliador J. Ghenciu , Nathaniel T. Becker , Landon M. Steele , Alan J. Russell
发明人： Eric J. Beckman , Eliador J. Ghenciu , Nathaniel T. Becker , Landon M. Steele , Alan J. Russell
IPC分类号： A23J100
CPC分类号： C12N9/54 , C07K1/145 , C07K14/465 , Y02P20/544 , Y10S435/814
摘要： There is provided a process for the extraction of water soluble biomaterials such as enzymes or proteins into carbon dioxide utilizing certain carbon dioxide-soluble surfactants. Also provided are certain carbon dioxide-soluble surfactants useful in the extraction of proteins. The surfactants are selected from fluoroether sulfate, fluoroether-polyethylene glycol block copolymer, fluoroether-functional sorbitol, and fluoroether dithiocarbamate chelate.
摘要翻译： 提供了利用某些二氧化碳可溶性表面活性剂将水溶性生物材料如酶或蛋白质提取到二氧化碳中的方法。 还提供了可用于提取蛋白质的某些二氧化碳可溶性表面活性剂。 表面活性剂选自氟醚硫酸盐，氟醚聚乙二醇嵌段共聚物，氟代醚官能山梨糖醇和氟醚二硫代氨基甲酸盐螯合物。
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公开(公告)号：US5879920A
公开(公告)日：1999-03-09
申请号：US577894
申请日：1995-12-22
IPC分类号： C11D1/29 , C11D1/72 , C11D3/37 , C11D3/386 , C11D17/00 , C12N11/02 , C12N11/04 , C12N11/14 , C12N9/98 , C11D10/00 , C12N11/08 , D06M16/00
CPC分类号： C11D3/3707 , C11D17/0039 , C11D3/3753 , C11D3/3776 , C11D3/38672 , C12N11/02 , C12N11/04 , C12N11/14 , C11D1/29 , C11D1/72
摘要： A granular enzyme composition is produced having reduced tendency to form dust and leave a residue, and improved stability and delayed release characteristics. The composition has a core, optionally coated with a vinyl polymer, a layer containing an enzyme and a vinyl polymer and optionally a plasticizer or anti-agglomeration agent, and an outer coating containing a polymer and optionally a low residue pigment and/or lubricant. Preferably, the core is a salt or sugar nonpareil, the vinyl polymer coating the core is polyvinyl alcohol and most preferably partially hydrolyzed polyvinyl alcohol, the vinyl polymer in the enzyme layer is polyvinyl pyrrolidone, and the polymer of the outer coating is polyvinyl pyrrolidone, polyvinyl alcohol which may be partially hydrolyzed, polyethylene glycol or mixtures thereof such as a mixture of polyvinyl pyrrolidone and polyvinyl alcohol or a mixture of polyvinyl pyrrolidone and polyethylene glycol. Preferably, the pigment is titanium dioxide and the lubricant is a nonionic or anionic surfactant such as a linear primary alcohol of a 9-15 carbon atom chain length alkane or alkene or an ethoxylate or ethoxysulfate derivative thereof. The enzyme can be a protease, amylase, lipase, cellulase, xylanase, oxidase, peroxidase or mixtures thereof. The composition may be produced by spray coating in a fluidized bed a solution of the enzyme and vinyl polymer onto the core to form the enzyme layer, and then spray coating in a fluidized bed a solution of polymer on the enzyme layer to form the outer coating.
摘要翻译： 产生颗粒状酶组合物，其具有降低形成粉尘并留下残留物的倾向，以及改进的稳定性和延迟释放特性。 该组合物具有核心，任选地涂覆有乙烯基聚合物，含有酶和乙烯基聚合物的层以及任选的增塑剂或抗附聚剂，以及含有聚合物和任选的低残留颜料和/或润滑剂的外涂层。 优选地，核心是盐或糖不分散体，涂覆核心的乙烯基聚合物是聚乙烯醇，最优选部分水解的聚乙烯醇，酶层中的乙烯基聚合物是聚乙烯吡咯烷酮，外涂层的聚合物是聚乙烯吡咯烷酮， 可以部分水解的聚乙烯醇，聚乙二醇或其混合物，例如聚乙烯吡咯烷酮和聚乙烯醇的混合物，或聚乙烯吡咯烷酮和聚乙二醇的混合物。 优选地，颜料是二氧化钛，润滑剂是非离子或阴离子表面活性剂，例如9-15碳原子的链烷烃或链烯烃的直链伯醇或其乙氧基化物或乙氧基硫酸盐衍生物。 酶可以是蛋白酶，淀粉酶，脂肪酶，纤维素酶，木聚糖酶，氧化酶，过氧化物酶或其混合物。 组合物可以通过在流化床中将酶和乙烯基聚合物的溶液喷雾到芯上以形成酶层，然后在流化床中将聚合物溶液在酶层上喷涂以形成外涂层 。
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