公开(公告)号：US07049151B2

公开(公告)日：2006-05-23

申请号：US10092926

申请日：2002-03-06

Applicant: Quan Nguyen ,  Yong Song

Inventor： Quan Nguyen ,  Yong Song

IPC: G01N33/543

CPC classification number: G01N33/6842 ,  G01N33/6803 ,  G01N33/6854 ,  Y10S435/973

Abstract: A method and kit for simultaneous detection and/or determination of a plurality of modified proteins in a sample. The method comprises: a) contacting the sample under mild protein denaturation conditions with a plurality of first antibodies capable of binding to a specific target protein, the first antibodies being immobilized on solid support material, each first antibody being differentiable from others by a differentiation parameter, whereby the first antibodies bind to respective target proteins present in the sample; b) removing unbound materials from the locus of the first antibodies; c) contacting the materials from step (b) with one or more second antibodies, each of which is specific to a class or subclass of modified proteins or with a plurality of second antibodies, each of which is specific to a modified protein, so as to bind the second antibody or antibodies to modified proteins in the sample; and d) detecting and/or determining a plurality of modified proteins in the sample. The kit comprises a plurality of primary antibodies immobilized on solid support material, one or more buffers for lysing and for washing cellular material samples to be assayed, an assay buffer for conducting the assay, the buffer containing a sulfate or sulfonate detergent, and one or more second antibodies specific to the modified proteins.

Abstract translation: 用于同时检测和/或测定样品中多种修饰蛋白质的方法和试剂盒。 该方法包括：a）将温和蛋白质变性条件下的样品与能够结合特异性靶蛋白的多个第一抗体接触，将第一抗体固定在固体支持材料上，每个第一抗体通过分化参数与其它抗体不同 ，其中第一抗体结合存在于样品中的各个靶蛋白质; b）从第一抗体的位点去除未结合的物质; c）使来自步骤（b）的材料与一种或多种第二抗体接触，每种抗体对修饰蛋白质的一类或亚类具有特异性，或者具有多种第二抗体，每种抗体对于修饰的蛋白质是特异性的，因此 将第二抗体或抗体与样品中的修饰蛋白结合; 和d）检测和/或确定样品中的多种修饰的蛋白质。 该试剂盒包含固定在固体支持材料上的多个一抗，一个或多个用于裂解和洗涤要测定的细胞材料样品的缓冲液，用于进行测定的测定缓冲液，含有硫酸盐或磺酸盐洗涤剂的缓冲液，以及一种或 更多的第二种对修饰蛋白特异的抗体。

公开(公告)号：US20060017492A1

公开(公告)日：2006-01-26

申请号：US10898787

申请日：2004-07-26

Applicant: Douglas Scratchley ,  Quan Nguyen ,  Ernest Graetz

Inventor： Douglas Scratchley ,  Quan Nguyen ,  Ernest Graetz

IPC: G05F1/10

CPC classification number: H02M3/07

Abstract: A voltage reference circuit receives an input voltage through a first port and a time varying input signal through a second port. The voltage reference circuit includes a switching circuit that is responsive to the first and the second ports and that generates an AC signal from the input voltage. The voltage reference circuit further includes a voltage multiplier circuit, coupled to switching circuit that receives the AC signal and creates a DC signal with a selected voltage level. The voltage reference circuit further includes a voltage regulator, coupled to the voltage multiplier circuit that regulates the DC signal from the voltage multiplier circuit. An output voltage is provided through an output port.

Abstract translation: 电压基准电路通过第一端口接收输入电压，并通过第二端口接收时变输入信号。 电压参考电路包括响应于第一和第二端口并且从输入电压产生AC信号的开关电路。 电压参考电路还包括电压倍增器电路，耦合到接收AC信号并产生具有所选电压电平的DC信号的开关电路。 电压参考电路还包括电压调节器，其耦合到调节来自电压倍增器电路的DC信号的电压倍增器电路。 通过输出端口提供输出电压。

公开(公告)号：US20050217899A1

公开(公告)日：2005-10-06

申请号：US10816542

申请日：2004-04-01

Applicant: Syed Hossain ,  James Larsen ,  Dwayne Terracina ,  Quan Nguyen

Inventor： Syed Hossain ,  James Larsen ,  Dwayne Terracina ,  Quan Nguyen

IPC: E21B7/00 ,  E21B10/00 ,  E21B10/16 ,  E21B10/18 ,  E21B17/00 ,  E21B19/00

CPC classification number: E21B10/16 ,  E21B10/18

Abstract: A drill bit configures hydraulic streams from the bit nozzles for the particular cutting structure to be cleaned based on the cutting structure location and geometry. The projected impingement location for each hydraulic stream projected fluid path is customized for a corresponding roller cone, with each nozzle or nozzle receptacle being individually positioned according to improve cone cleaning. At least two nozzles or nozzle receptacles should differ on the drill bit.

Abstract translation: 基于切割结构位置和几何形状，钻头针对待清洁的特定切割结构从钻头喷嘴构造液压流。 每个液压流投影流体路径的预计冲击位置是针对相应的滚子锥定制的，每个喷嘴或喷嘴容器根据改进的锥体清洁单独定位。 钻头上至少有两个喷嘴或喷嘴容器应有所不同。