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    Oscillating Amplification Reaction for Nucleic Acids
    21.
    发明申请
    Oscillating Amplification Reaction for Nucleic Acids 有权
    核酸振荡扩增反应

    公开(公告)号：US20140045221A1

    公开(公告)日：2014-02-13

    申请号：US14113200

    申请日：2012-04-20

    申请人： Hong Cai ,  Nathan J. Cobb

    发明人： Hong Cai ,  Nathan J. Cobb

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/686 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q2522/101 ,  C12Q2525/204 ,  C12Q2527/107 ,  C12Q2527/125 ,  C12Q2527/127

    摘要： One embodiment of the present invention provides for a method for amplifying a template of nucleic acid target sequence contained in a sample. The method includes contacting the sample with an amplification reaction mixture containing a primer complementary to the template of nucleic acid target sequence. A temperature of the reaction is oscillated between an upper temperature and a lower temperature wherein the change in temperature is no greater than about 20° C. during a plurality of temperature cycles. The template of nucleic acid target sequence is amplified.

    摘要翻译： 本发明的一个实施方案提供了扩增样品中所含的核酸靶序列的模板的方法。 该方法包括使样品与含有与核酸靶序列模板互补的引物的扩增反应混合物接触。 在多个温度循环期间，反应温度在上限温度和较低温度之间振荡，其中温度变化不大于约20℃。 扩增核酸靶序列模板。

    Compositions And Methods For The Rapid Detection Of Legionella pneumophila
    22.
    发明申请
    Compositions And Methods For The Rapid Detection Of Legionella pneumophila 审中-公开
    用于快速检测嗜肺军团菌的组合物和方法

    公开(公告)号：US20130011831A1

    公开(公告)日：2013-01-10

    申请号：US12935131

    申请日：2010-03-25

    申请人： Jing Luo ,  Hong Cai ,  Jing Chen

    发明人： Jing Luo ,  Hong Cai ,  Jing Chen

    IPC分类号： C07H21/04 ,  G01N21/64 ,  C12Q1/68

    CPC分类号： C12Q1/689 ,  Y02A50/451

    摘要： The present application describes compositions and methods useful for the rapid detection of Legionella pneumophila. The compositions include capture probes, amplification primers, primer sets and detection probes that comprise nucleic acid molecules that hybridize to L. pneumophila 23S rRNA or DNA encoding 23S rRNA target sequences. Also described are methods for detecting and/or quantifying the amount of L. pneumophila in a sample using real time PCR (rPCR) or revere transcriptase real time PCR (RT-rPCR).

    摘要翻译： 本申请描述了用于快速检测嗜肺军团菌的组合物和方法。 组合物包括捕获探针，扩增引物，引物组和检测探针，其包含与嗜肺军团菌23S rRNA或编码23S rRNA靶序列的DNA杂交的核酸分子。 还描述了使用实时PCR（rPCR）或重复转录酶实时PCR（RT-rPCR）检测和/或定量样品中嗜肺军团杆菌的量的方法。

    Keeping persistence while switching between modalities when accessing an application service in a network
    23.
    发明授权
    Keeping persistence while switching between modalities when accessing an application service in a network 失效
    在访问网络中的应用程序服务时，在切换模式之间保持持久性

    公开(公告)号：US07925763B2

    公开(公告)日：2011-04-12

    申请号：US10486716

    申请日：2002-07-08

    申请人： Hong Cai

    发明人： Hong Cai

    IPC分类号： G06F15/16

    CPC分类号： G06F9/485 ,  G06F2209/482

    摘要： Disclosed is a method and device for keeping persistency while switching between modalities, which include: (1) searching out the last operation state of the application service accessed by the user from the user history, in response to a request for accessing an application service from a user; (2) connecting to the application service and continuing to execute the application service from the last operation state; (3) updating the last operation state in the user history based on the execution result of the application service. A user could flexibly selects different favorable terminal to continue with unfinished application service by adopting the invention.

    摘要翻译： 公开了一种用于在切换模式之间保持持续性的方法和装置，其包括：（1）响应于从用户历史访问请求访问应用服务的请求，从用户历史中查找访问的应用服务的最后操作状态 用户; （2）连接到应用服务，并从上一个操作状态继续执行应用服务; （3）基于应用服务的执行结果更新用户历史中的最后操作状态。 通过采用本发明，用户可灵活选择不同的有利终端继续未完成的应用服务。

    TOTAL BACTERIA MONITORING SYSTEM
    24.
    发明申请
    TOTAL BACTERIA MONITORING SYSTEM 有权
    总细菌监测系统

    公开(公告)号：US20100112682A1

    公开(公告)日：2010-05-06

    申请号：US12472024

    申请日：2009-05-26

    申请人： Scott M. Boyette ,  Hong Cai ,  Yan Jin ,  Jie Li ,  Kechao Yang ,  Rong Xu ,  Yu Wang

    发明人： Scott M. Boyette ,  Hong Cai ,  Yan Jin ,  Jie Li ,  Kechao Yang ,  Rong Xu ,  Yu Wang

    IPC分类号： C12M1/00

    CPC分类号： C12Q1/04 ,  G01N21/274 ,  G01N21/6486 ,  G01N2021/6439

    摘要： A system for measuring total microbiological content in an aqueous medium by adding a fluorescent dye to the aqueous medium, measuring the fluorescent signal in the aqueous medium to obtain a baseline fluorescent signal, releasing intracellular content of the microbiological matter into the aqueous medium by lysing the microbiological matter. The system then measures the fluorescent signal in the aqueous medium with the released intracellular content of the microbiological matter to obtain a second fluorescent signal. Next, the system subtracts the baseline signal from the second fluorescent signal to obtain a net fluorescent signal and equates the net fluorescent signal with a microbiological content.

    摘要翻译： 一种用于通过向水介质中加入荧光染料来测量水性介质中的总微生物含量的系统，测量水性介质中的荧光信号以获得基线荧光信号，通过裂解所述微生物物质将微生物物质释放到水介质中 微生物。 然后，系统在微生物物质释放的细胞内含量的水介质中测量荧光信号以获得第二荧光信号。 接下来，系统从第二荧光信号中减去基线信号以获得净荧光信号，并将净荧光信号与微生物含量相等。

    Placement-Driven Physical-Hierarchy Generation
    25.
    发明申请
    Placement-Driven Physical-Hierarchy Generation 审中-公开
    放置驱动的物理层次生成

    公开(公告)号：US20070245281A1

    公开(公告)日：2007-10-18

    申请号：US11734757

    申请日：2007-04-12

    申请人： Michael Riepe ,  Niranjana Balasundaram ,  Menno Verbeek ,  Hong Cai ,  Roger Carpenter ,  Jacob Avidan

    发明人： Michael Riepe ,  Niranjana Balasundaram ,  Menno Verbeek ,  Hong Cai ,  Roger Carpenter ,  Jacob Avidan

    IPC分类号： G06F17/50 ,  G06F9/45

    CPC分类号： G06F17/5072

    摘要： A method and system for performing placement-driven physical hierarchy generation in the context of an integrated circuit layout generation system is provided. This generation optimizes the physical hierarchy to improve placement of the cells in the layout, and the associated interconnect routability and delay. A new pre-clustering phase is introduced to maintain as much of the input logical hierarchy as possible while maintaining physical hierarchy quality. And a new cost function is described which is based on measuring the mutual affinity of cells in a virtually-flat placement. The new cost function is used during the new pre-clustering phase, as well as the common clustering, partitioning, and declustering/refinement phases of physical hierarchy generation.

    摘要翻译： 提供了一种用于在集成电路布局生成系统的上下文中执行布置驱动物理层次生成的方法和系统。 这一代优化了物理层次结构，以改善布局中单元格的布局，以及相关联的互连可布线性和延迟。 引入了一个新的预聚类阶段，以保持尽可能多的输入逻辑层次结构，同时保持物理层次质量。 并且描述了新的成本函数，其基于测量细胞在几乎平坦的位置中的相互亲和力。 新的成本函数在新的聚类前阶段使用，以及物理层次生成的公共聚类，分割和分解/细化阶段。

    Quenching methods for background reduction in luminescence-based probe-target binding assays
    26.
    发明授权
    Quenching methods for background reduction in luminescence-based probe-target binding assays 失效
    用于基于发光的探针 - 靶结合测定法的背景降低的淬灭方法

    公开(公告)号：US07202036B2

    公开(公告)日：2007-04-10

    申请号：US10460451

    申请日：2003-06-12

    申请人： Hong Cai ,  Peter M Goodwin ,  Richard A. Keller ,  Rhiannon L. Nolan

    发明人： Hong Cai ,  Peter M Goodwin ,  Richard A. Keller ,  Rhiannon L. Nolan

    IPC分类号： C12Q1/68

    CPC分类号： G01N33/542 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6832 ,  C12Q2600/156 ,  C12Q2565/101 ,  C12Q2563/107 ,  C12Q2565/1015 ,  C12Q2521/301

    摘要： Background luminescence is reduced from a solution containing unbound luminescent probes, each having a first molecule that attaches to a target molecule and having an attached luminescent moiety, and luminescent probe/target adducts. Quenching capture reagent molecules are formed that are capable of forming an adduct with the unbound luminescent probes and having an attached quencher material effective to quench luminescence of the luminescent moiety. The quencher material of the capture reagent molecules is added to a solution of the luminescent probe/target adducts and binds in a proximity to the luminescent moiety of the unbound luminescent probes to quench luminescence from the luminescent moiety when the luminescent moiety is exposed to exciting illumination. The quencher capture reagent does not bind to probe molecules that are bound to target molecules and the probe/target adduct emission is not quenched.

    摘要翻译： 从含有未结合的发光探针的溶液中减少背景发光，每个发光探针具有连接到靶分子并具有连接的发光部分的第一分子和发光探针/靶加合物。 形成捕获试剂分子，其能够与未结合的发光探针形成加合物，并具有有效地淬灭发光部分的发光的连接的淬灭剂材料。 将捕获试剂分子的猝灭剂材料加入到发光探针/靶加合物的溶液中，并且在未结合的发光探针的发光部分附近结合，以在发光部分暴露于激发照明时淬灭发光部分的发光 。 猝灭剂捕获试剂不结合到与靶分子结合的探针分子，并且探针/靶加合物发射不被淬灭。

    Oscillating amplification reaction for nucleic acids
    27.
    发明授权
    Oscillating amplification reaction for nucleic acids 有权
    核酸的振荡扩增反应

    公开(公告)号：US09428781B2

    公开(公告)日：2016-08-30

    申请号：US14113200

    申请日：2012-04-20

    申请人： Hong Cai ,  Nathan J. Cobb

    发明人： Hong Cai ,  Nathan J. Cobb

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/686 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q2522/101 ,  C12Q2525/204 ,  C12Q2527/107 ,  C12Q2527/125 ,  C12Q2527/127

    摘要： One embodiment of the present invention provides for a method for amplifying a template of nucleic acid target sequence contained in a sample. The method includes contacting the sample with an amplification reaction mixture containing a primer complementary to the template of nucleic acid target sequence. A temperature of the reaction is oscillated between an upper temperature and a lower temperature wherein the change in temperature is no greater than about 20° C. during a plurality of temperature cycles. The template of nucleic acid target sequence is amplified.

    摘要翻译： 本发明的一个实施方案提供了扩增样品中所含的核酸靶序列的模板的方法。 该方法包括使样品与含有与核酸靶序列模板互补的引物的扩增反应混合物接触。 在多个温度循环期间，反应温度在上限温度和较低温度之间振荡，其中温度变化不大于约20℃。 扩增核酸靶序列模板。

    Remote servlets collaboration
    29.
    发明授权
    Remote servlets collaboration 有权
    远程servlet协作

    公开(公告)号：US08387042B2

    公开(公告)日：2013-02-26

    申请号：US12142331

    申请日：2008-06-19

    申请人： Hong Cai ,  Wei Lu ,  Li Hua Tang ,  Bo Yang

    发明人： Hong Cai ,  Wei Lu ,  Li Hua Tang ,  Bo Yang

    IPC分类号： G06F9/455 ,  G06F9/44

    CPC分类号： H04L61/1558 ,  H04L29/06 ,  H04L29/12141 ,  H04L67/02 ,  H04L67/10 ,  H04L69/329

    摘要： Presents remote servlets collaboration. A method includes: creating a common registry; creating an extended context in response to an external request, wherein the extended context can invoke the resources within the common registry; performing initializing configuration on a plurality of servlets, and registering a plurality of servlets in the common registry, wherein a plurality of servlets being located on different Java virtual machines, or being located in different servlet containers on the same Java virtual machine; and a plurality of servlets collaborate with each other by being invoked through the extended context. It can implement collaboration between servlets located on different virtual machines or even servlets on the same virtual machine but within different servlet containers, because in a converged application service system, not only between HTTP servlets or between SIP servlets but also between HTTP servlets and SIP servlets there is necessity to collaborate.

    摘要翻译： 呈现远程servlet协作。 一种方法包括：创建公共注册表; 响应于外部请求创建扩展上下文，其中所述扩展上下文可以调用所述公共注册表中的资源; 在多个servlet上执行初始化配置，以及将多个servlet注册在所述公共注册表中，其中多个servlet位于不同的Java虚拟机上，或位于同一Java虚拟机上的不同servlet容器中; 并且通过通过扩展上下文调用多个servlet彼此协作。 因为在融合的应用服务系统中，不仅在HTTP servlet之间或者在SIP servlet之间，而且在HTTP servlet和SIP servlet之间，它可以实现位于不同虚拟机上的servlet之间的协同，甚至在同一个虚拟机上的Servlet， 有必要合作。