公开(公告)号：US08697361B2

公开(公告)日：2014-04-15

申请号：US12919905

申请日：2009-02-27

申请人： Bankole A. Johnson

发明人： Bankole A. Johnson

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02

CPC分类号： C12Q1/6883 ,  A61K31/4178 ,  A61K45/06 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/6872 ,  G01N33/942 ,  G01N2800/307 ,  G01N2800/50

摘要： The gene responsible for encoding SERT has a functional polymorphism at the 5′-regulatory promoter region, which results in two forms, long (L) and short (S). The LL-genotype is hypothesized to play a key role in the early onset of alcohol use. The present invention discloses the differences in treatment and diagnosis based on the L or short genotypes as well as on a single nucleotide polymorphism of the SERT gene, the 3′ UTR SNP rs 1042173. The present invention demonstrates the efficacy of using the drug ondansetron and similar drugs for treatment based on variations in the polymorphisms of the SERT gene as well as methods for diagnosing susceptibility to abuse of alcohol and other addiction-related diseases and disorders.

摘要翻译： 负责编码SERT的基因在5'调节启动子区具有功能多态性，其形成长（L）和短（S）两种形式。 假设LL型基因型在酒精使用早期发挥关键作用。 本发明公开了基于L或短基因型的治疗和诊断的差异以及SERT基因，3'UTR SNP rs 1042173的单核苷酸多态性的差异。本发明证明了使用丹参酮和 基于SERT基因多态性变化的治疗类似药物以及用于诊断酒精和其他成瘾相关疾病和障碍滥用易感性的方法。

公开(公告)号：US08647825B2

公开(公告)日：2014-02-11

申请号：US12987425

申请日：2011-01-10

申请人： Kent E. Vrana ,  Willard M. Freeman ,  Kathleen A. Grant ,  Steve Gonzales

发明人： Kent E. Vrana ,  Willard M. Freeman ,  Kathleen A. Grant ,  Steve Gonzales

IPC分类号： G01N33/53 ,  A61K49/00

CPC分类号： G01N33/6893 ,  C12Q1/6883 ,  C12Q2600/142 ,  G01N2800/307

摘要： Methods and compositions according to embodiments of the present invention are provided that specifically and sensitively detect alcohol consumption and whether alcohol consumption is moderate or high in a subject. Aspects of the present invention relate to assays of panels of proteins for detecting non-consumption, moderate consumption and high consumption of ethanol by a subject.

摘要翻译： 提供了根据本发明实施方案的方法和组合物，其特异性且敏感地检测酒精消耗以及受试者的饮酒量是否适中或高。 本发明的方面涉及用于检测受试者的非消费，中度消耗和高消耗乙醇的蛋白质组的测定。

公开(公告)号：US20110112159A1

公开(公告)日：2011-05-12

申请号：US12919905

申请日：2009-02-27

申请人： Bankole A. Johnson

发明人： Bankole A. Johnson

IPC分类号： A61K31/4178 ,  C12Q1/68 ,  A61P25/32

CPC分类号： C12Q1/6883 ,  A61K31/4178 ,  A61K45/06 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/6872 ,  G01N33/942 ,  G01N2800/307 ,  G01N2800/50

摘要： The gene responsible for encoding SERT has a functional polymorphism at the 5′-regulatory promoter region, which results in two forms, long (L) and short (S). The LL-genotype is hypothesized to play a key role in the early onset of alcohol use. The present invention discloses the differences in treatment and diagnosis based on the L or short genotypes as well as on a single nucleotide polymorphism of the SERT gene, the 3′ UTR SNP rs 1042173. The present invention demonstrates the efficacy of using the drug ondansetron and similar drugs for treatment based on variations in the polymorphisms of the SERT gene as well as methods for diagnosing susceptibility to abuse of alcohol and other addiction-related diseases and disorders.

摘要翻译： 负责编码SERT的基因在5'调节启动子区具有功能多态性，其形成长（L）和短（S）两种形式。 假设LL型基因型在酒精使用早期发挥关键作用。 本发明公开了基于L或短基因型的治疗和诊断的差异以及SERT基因，3'UTR SNP rs 1042173的单核苷酸多态性的差异。本发明证明了使用丹参酮和 基于SERT基因多态性变化的治疗类似药物以及用于诊断酒精和其他成瘾相关疾病和病症滥用易感性的方法。

公开(公告)号：US20030175993A1

公开(公告)日：2003-09-18

申请号：US10278392

申请日：2002-10-23

发明人： Anthony Toranto ,  Evan Singer ,  Brett Miller

IPC分类号： G01N033/543

CPC分类号： G01N33/487 ,  A61B10/0051 ,  A61B2010/0006 ,  A61B2010/0009 ,  G01N2800/307

摘要： The present invention relates to analyte detection test systems, including test systems for the oral detection of analytes in saliva. The present invention also provides compositions and methods for storing multiple assay tests and compositions and methods for measuring the concentration of analytes in a sample.

摘要翻译： 本发明涉及分析物检测测试系统，包括用于口腔检测唾液中分析物的测试系统。 本发明还提供用于储存多个测定试验的组合物和方法以及用于测量样品中分析物浓度的组合物和方法。

公开(公告)号：US06620107B2

公开(公告)日：2003-09-16

申请号：US10025544

申请日：2001-12-18

申请人： Peter Alfred Payne ,  Krishna Chandra Persaud ,  Allan John Syms

发明人： Peter Alfred Payne ,  Krishna Chandra Persaud ,  Allan John Syms

IPC分类号： A61B500

CPC分类号： G01N33/497 ,  C12Q1/04 ,  G01N33/6893 ,  G01N33/98 ,  G01N2800/307

摘要： A method for detecting the occurrence of a condition in a respiring subject includes the steps of: introducing emitted subject respiratory gases to a gas sensing device; detecting certain species present in the emitted subject respiratory gases with the gas sensing device; and correlating the presence of the species with the occurrence of the condition.

摘要翻译： 一种用于检测呼吸对象中的状况的发生的方法包括以下步骤：将排出的受试呼吸气体引入气体感测装置; 用气体检测装置检测发射的受试呼吸气体中存在的某些物质; 并将物种的存在与条件的发生相关联。

公开(公告)号：US20020151814A1

公开(公告)日：2002-10-17

申请号：US10025544

申请日：2001-12-18

申请人： Osmetech Plc, a United Kingdom corporation

发明人： Peter Alfred Payne ,  Krishna Chandra Persaud ,  Allan John Syms

IPC分类号： A61B005/08

CPC分类号： G01N33/497 ,  C12Q1/04 ,  G01N33/6893 ,  G01N33/98 ,  G01N2800/307

摘要： nullThere is disclosed a method for detecting the occurrence of a condition in a respiring subject comprising the steps of: introducing emitted subject respiratory gases to a gas sensing device; detecting certain species present in said emitted subject respiratory gases with said gas sensing device; and correlating the presence of said species with the occurrence of the conditionnull A method for detecting the occurrence of a condition in a respiring subject includes the steps of: introducing emitted subject respiratory gases to a gas sensing device; detecting certain species present in the emitted subject respiratory gases with the gas sensing device; and correlating the presence of the species with the occurrence of the condition.

摘要翻译： [公开了一种用于检测呼吸对象中病症发生的方法，包括以下步骤：将排出的受试呼吸气体引入气体感测装置; 用所述气体感测装置检测存在于所述排出的受试呼吸气体中的某些物质; 并且将所述物种的存在与条件的发生相关联。一种用于检测呼吸对象中的病症发生的方法，包括以下步骤：将排出的受试呼吸气体引入气体感测装置; 用气体检测装置检测发射的受试呼吸气体中存在的某些物质; 并将物种的存在与条件的发生相关联。

公开(公告)号：US5866427A

公开(公告)日：1999-02-02

申请号：US873896

申请日：1997-06-13

申请人： Jack H. Mendelson ,  Nancy K. Mello ,  Tak-Ming Chiu

发明人： Jack H. Mendelson ,  Nancy K. Mello ,  Tak-Ming Chiu

IPC分类号： G01N24/08 ,  G01N33/48 ,  G01N33/487 ,  G01N33/49 ,  G01N33/497 ,  G01N33/98 ,  G01R33/465

CPC分类号： G01N24/08 ,  G01N33/491 ,  G01N33/4972 ,  G01N33/98 ,  G01R33/465 ,  G01N2800/307 ,  G01N33/487 ,  G01N33/48707 ,  Y10T436/203332 ,  Y10T436/204165 ,  Y10T436/24

摘要： In the method of the invention, in vitro proton MRS is employed to determine the measurable ethanol concentrations in ethanol-treated erythrocyte samples of occasional and heavy drinkers. An erythrocyte to plasma ethanol concentration ratio is determined based upon the MRS measured erythrocyte concentration. Erythrocyte to plasma ethanol concentration ratios are significantly greater for heavy drinkers (a subset of alcohol tolerant individuals) as compared to the ratios calculated for occasional drinkers (non-tolerant individuals) when erythrocyte ethanol concentration is determined by MRS. Thus, the method of the present invention discriminates between alcohol tolerant individuals and alcohol non-tolerant individuals based upon the magnitude of the erythrocyte to plasma ethanol concentration ratio obtained according to the methods disclosed herein.

摘要翻译： 在本发明的方法中，使用体外质子MRS来测定偶尔和重度饮酒者的乙醇处理的红细胞样品中可测量的乙醇浓度。 红细胞与血浆乙醇的浓度比是根据MRS测定的红细胞浓度来确定的。 与通过MRS测定红细胞乙醇浓度时偶尔饮酒者（非耐受性个体）计算的比例相比，重度饮酒者（酒精耐受性个体的亚型）的红细胞对血浆乙醇浓度比显着更大。 因此，本发明的方法基于根据本文公开的方法获得的红细胞与血浆乙醇浓度比的大小，区分耐酒精个体和不耐受酒精的个体。

公开(公告)号：US5747346A

公开(公告)日：1998-05-05

申请号：US259730

申请日：1994-05-27

申请人： Raju K. Pullarkat ,  Premila S. Pullarkat ,  Simhachalam Raguthu

发明人： Raju K. Pullarkat ,  Premila S. Pullarkat ,  Simhachalam Raguthu

IPC分类号： G01N30/90 ,  G01N33/66 ,  G01N33/98 ,  G01N33/00 ,  G01N21/77 ,  G01N30/02

CPC分类号： G01N33/66 ,  G01N33/98 ,  G01N2800/307 ,  G01N30/90 ,  Y10T436/142222 ,  Y10T436/143333 ,  Y10T436/20 ,  Y10T436/201666 ,  Y10T436/203332

摘要： Chronic or long-term alcohol consumption is detected and monitored by determining the level of a newly-observed, alcohol-specific carbohydrate in body fluids (e.g. urine) of subjects by calorimetric reaction using qualitative and quantitative assay methods. The alcohol-specific carbohydrate have been identified as a novel ethyl glucuronide. Ethyl glucuronide is observed and detected in direct response to alcohol consumption in body fluids, and can be isolated and purified. Simple, economical, and reproducible assay methods, such as a spot assay and an ascending or thin layer chromatography assay, provide reliable, objective, and sensitive methods for detecting and monitoring a chronic alcoholic condition. Both the presence of the alcohol-specific ethyl glucuronide and a substantial increase in its levels are indicative of chronic alcoholism. Since the novel ethyl glucuronide is produced and appears as a direct response to chronic alcohol intake, the novel carbohydrate is considered to be a unique biomarker for the detection of alcoholism, with virtually no possibility of false positive results.

摘要翻译： 通过使用定性和定量测定方法通过量热反应确定受试者的体液（例如尿）中新观察到的酒精特异性碳水化合物的水平来检测和监测慢性或长期饮酒。 酒精特异性碳水化合物已被鉴定为新型乙基葡糖苷酸。 葡萄糖醛酸葡萄糖醛酸苷在体液中的酒精消耗直接反应中被观察和检测，并且可以被分离和纯化。 简单，经济和可重现的测定方法，如斑点测定和上升或薄层色谱测定，为检测和监测慢性酒精状况提供可靠，客观和敏感的方法。 酒精特异性葡萄糖醛酸乙酯的存在和其水平的显着增加都表明慢性酒精中毒。 由于产生新的乙基葡糖苷酸，并且显示为对慢性酒精摄入的直接反应，所以新型碳水化合物被认为是用于检测酒精中毒的独特生物标志物，实际上没有假阳性结果的可能性。

公开(公告)号：US5374562A

公开(公告)日：1994-12-20

申请号：US853672

申请日：1992-03-19

申请人： Wilhelm Simon

发明人： Wilhelm Simon

IPC分类号： C07C233/25 ,  C07C233/33 ,  C07D277/50 ,  G01N33/98 ,  G01N33/14

CPC分类号： G01N33/98 ,  C07C233/25 ,  C07C233/33 ,  C07D277/50 ,  G01N2800/307 ,  Y10T436/20 ,  Y10T436/203332 ,  Y10T436/204165 ,  Y10T436/255

摘要： Hemiacetals are disclosed which correspond to the following formula II ##STR1## in which R' is an aliphatic or cycloaliphatic residue,Ar is an unsubstituted or substituted monocyclic or polycyclic aromatic or heteroaromatic residue andX.sup.1, X.sup.2 and X.sup.3 are selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, fluoro, chloro, bromo and nitro, wherein however at least one of said substituents has to be selected from the stated halo atoms or nitro. The hemiacetals are formed reversibly from an alcohol and a corresponding keto compound and the hemiacetals of formula II differ from the corresponding keto compounds as to their light absorption in the ultraviolet, visible or infrared range, or a fluorescence or luminescence is created or quenched when the keto compound is converted into the hemiacetal of formula II. Said reaction can be performed in sensors which are used for the optical determination of alcohols.

摘要翻译： 公开了半缩醛，其对应于下式II其中R'是脂族或脂环族残基，Ar是未取代或取代的单环或多环芳族或杂芳族残基，X 1，X 2和X 3选自 氢，烷基，烯基，炔基，氟，氯，溴和硝基，其中至少一个所述取代基必须选自所述卤素原子或硝基。 半缩醛由醇和相应的酮化合物可逆地形成，并且式II的半缩醛与相应的酮化合物在紫外线，可见光或红外光谱范围内的光吸收不同，或当荧光或发光产生或淬灭时 酮化合物转化为式II的半缩醛。 所述反应可以在用于醇的光学测定的传感器中进行。

公开(公告)号：US20180305754A1

公开(公告)日：2018-10-25

申请号：US15743836

申请日：2016-07-12

申请人： KOREA INSTITURE OF SCIENCE AND TECHNOLOGY

发明人： Heh-In IM

IPC分类号： C12Q1/6876 ,  G01N33/68

CPC分类号： C12Q1/6876 ,  C12Q1/68 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N33/53 ,  G01N33/6893 ,  G01N2800/307

摘要： The present disclosure relates to a biomarker for diagnosis of drug addiction and a kit for diagnosis of drug addiction. The biomarker for diagnosis of drug addiction and the kit for diagnosis of drug addiction according to the present disclosure can determine drug addiction by using a molecular biological method. Especially, the present disclosure has investigated molecular biologically how microRNA functions in the human brain due to drug addiction and how the microRNA is transferred to blood or serum by using an exosome as a means of transport. Therefore, the problem that, even though the change in the expression pattern of the microRNA in the brain is validated, it is difficult to apply the change in the expression pattern in the brain for clinical purposes directly and simply, was solved.