公开(公告)号：US07795503B2

公开(公告)日：2010-09-14

申请号：US11360459

申请日：2006-02-22

申请人： Nestor Apuya ,  Steven Craig Bobzin ,  Joon-Hyun Park

发明人： Nestor Apuya ,  Steven Craig Bobzin ,  Joon-Hyun Park

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8243 ,  C12N15/8216

摘要： Materials and methods are provided for identifying regulatory region-regulatory protein associations and modulating expression of a sequence of interest. For example, a plant cell is provided containing a regulatory protein that can modulate expression of one or more genes involved in alkaloid biosynthesis in plants, which, in turn, can modulate the amount and/or rate of biosynthesis of one or more alkaloid compounds.

摘要翻译： 提供材料和方法用于鉴定调节性区域调节蛋白关联并调节感兴趣的序列的表达。 例如，提供含有调节蛋白质的植物细胞，所述调节蛋白可以调节植物中参与生物碱生物合成的一种或多种基因的表达，其依次可以调节一种或多种生物碱化合物的生物合成的量和/或速率。

公开(公告)号：US20090133163A1

公开(公告)日：2009-05-21

申请号：US12103970

申请日：2008-04-16

申请人： Nestor Apuya ,  Shing Kwok ,  Nickolai Alexandrov ,  Tatiana Tatarinova ,  Yiwen Fang ,  Roger Pennell ,  Yu-Ping Lu ,  Leonard Medrano ,  Zhihong Cook ,  Kenneth Feldmann

发明人： Nestor Apuya ,  Shing Kwok ,  Nickolai Alexandrov ,  Tatiana Tatarinova ,  Yiwen Fang ,  Roger Pennell ,  Yu-Ping Lu ,  Leonard Medrano ,  Zhihong Cook ,  Kenneth Feldmann

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N15/63 ,  G01N33/50 ,  C12N5/10 ,  C12N15/87

CPC分类号： C12N15/8237 ,  C12N15/8225 ,  C12N15/8226 ,  C12N15/8227 ,  C12N15/8229 ,  C12N15/823 ,  C12N15/8233 ,  C12N15/8234 ,  Y10T436/143333

摘要： The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.

摘要翻译： 本发明涉及启动子序列和启动子控制元件，包含启动子和控制元件的多核苷酸构建体，以及鉴定启动子，控制元件或其片段的方法。 本发明还涉及本发明启动子或启动子控制元件调节转录水平的用途。

公开(公告)号：US20050204416A1

公开(公告)日：2005-09-15

申请号：US11035623

申请日：2005-01-14

申请人： Richard Hamilton ,  Nestor Apuya ,  Steven Bobzin

发明人： Richard Hamilton ,  Nestor Apuya ,  Steven Bobzin

IPC分类号： A01H1/00 ,  C12N5/04 ,  C12N15/82 ,  C12Q1/00 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/50

CPC分类号： C12N15/8238 ,  C12N15/8217 ,  C12N15/8257 ,  G01N33/5097 ,  G01N2333/70567 ,  G01N2333/72

摘要： Disclosed are novel transgenic plant cells that include a heterologous (e.g., human) receptor polypeptide, or fragment thereof. The plant cells can be used to identify molecules (i.e., ligands) that can interact with the receptor polypeptide or fragment. The plant cells can be used to identify ligands that are endogenous to transgenic plant cells, or exogenous ligands that are applied to the plant cells. Such receptor polypeptide ligands can be used to identify novel pharmaceuticals.

摘要翻译： 公开了包含异源（例如人）受体多肽或其片段的新型转基因植物细胞。 植物细胞可用于鉴定可与受体多肽或片段相互作用的分子（即配体）。 植物细胞可以用于鉴定转基因植物细胞内源的配体或施用于植物细胞的外源配体。 这种受体多肽配体可用于鉴定新型药物。

公开(公告)号：US09758790B2

公开(公告)日：2017-09-12

申请号：US13584421

申请日：2012-08-13

申请人： Steven Craig Bobzin ,  Nestor Apuya ,  Karen Chiang ,  Elena Doukhanina ,  Kenneth Feldmann ,  Boris Jankowski ,  Daniel Mumenthaler ,  Joon-Hyun Park ,  Amr Saad Ragab ,  Joel Cruz Rarang ,  Richard Schneeberger ,  Jennifer E. Van Fleet ,  Ke Zhang

发明人： Steven Craig Bobzin ,  Nestor Apuya ,  Karen Chiang ,  Elena Doukhanina ,  Kenneth Feldmann ,  Boris Jankowski ,  Emilio Margolles-Clark ,  Daniel Mumenthaler ,  Jack Okamuro ,  Joon-Hyun Park ,  Amr Saad Ragab ,  Joel Cruz Rarang ,  Richard Schneeberger ,  Jennifer E. Van Fleet ,  Ke Zhang

IPC分类号： C12N15/82 ,  C07K14/415

CPC分类号： C12N15/8241 ,  C07K14/415 ,  C12N15/8247 ,  C12N15/8255

摘要： Materials and Methods for identifying lignin regulatory region-regulatory protein associations are disclosed. Materials and methods for modulating lignin accumulation are also disclosed. In addition, methods and materials for modulating (e.g., increasing or decreasing) the level of a component (e.g., protein, oil, lignin, carbon, a carotenoid, or a triterpenoid) in plants are disclosed.

公开(公告)号：US09441233B2

公开(公告)日：2016-09-13

申请号：US13696506

申请日：2011-05-05

申请人： Nestor Apuya ,  Vijay Sharma ,  Chuan-Yin Wu

发明人： Nestor Apuya ,  Vijay Sharma ,  Chuan-Yin Wu

IPC分类号： C12N15/82 ,  C07K14/415 ,  C12N9/10 ,  C12Q1/68

CPC分类号： C12N15/8242 ,  C07K14/415 ,  C12N9/1048 ,  C12N15/8261 ,  C12Q1/6895 ,  C12Q2600/13 ,  Y02A40/146

摘要： Methods and materials for modulating biomass levels in plants are disclosed. For example, nucleic acids encoding biomass-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased biomass levels and plant products produced from plants having increased biomass levels.

摘要翻译： 公开了调节植物生物量的方法和材料。 例如，公开了编码生物质调节多肽的核酸以及使用这种核酸来转化植物细胞的方法。 还公开了具有增加的生物量水平的植物和由具有增加的生物量水平的植物产生的植物产物。

公开(公告)号：US09029523B2

公开(公告)日：2015-05-12

申请号：US11004678

申请日：2004-12-03

申请人： Nickolai Alexandrov ,  Nestor Apuya ,  Vyacheslav Brover ,  Jean-Baptiste Dumas ,  Yiwen Fang ,  Ken Feldmann ,  Diane Jofuku ,  Edward A. Kiegle ,  Bill Kimmerly ,  Shing Kwok ,  Peter Mascia ,  Jack Okamuro ,  Roger Pennell ,  Richard Schneeberger ,  Tatiana Tatarinova ,  Wayne Volkmuth

发明人： Nickolai Alexandrov ,  Nestor Apuya ,  Vyacheslav Brover ,  Jean-Baptiste Dumas ,  Yiwen Fang ,  Ken Feldmann ,  Diane Jofuku ,  Edward A. Kiegle ,  Bill Kimmerly ,  Shing Kwok ,  Peter Mascia ,  Jack Okamuro ,  Roger Pennell ,  Richard Schneeberger ,  Tatiana Tatarinova ,  Wayne Volkmuth

IPC分类号： C12N15/82 ,  C07H21/04 ,  C12N5/14 ,  C12N15/00 ,  A01H5/00 ,  C07K14/415

CPC分类号： C12N15/8216 ,  C07K14/415

摘要： The present invention provides DNA molecules that constitute fragments of the genome of a plant, and polypeptides encoded thereby. The DNA molecules are useful for specifying a gene product in cells, either as a promoter or as a protein coding sequence or as an UTR or as a 3′ termination sequence, and are also useful in controlling the behavior of a gene in the chromosome, in controlling the expression of a gene or as tools for genetic mapping, recognizing or isolating identical or related DNA fragments, or identification of a particular individual organism, or for clustering of a group of organisms with a common trait. One of ordinary skill in the art, having this data, can obtain cloned DNA fragments, synthetic DNA fragments or polypeptides constituting desired sequences by recombinant methodology known in the art or described herein.

摘要翻译： 本发明提供构成植物基因组片段的DNA分子，以及由此编码的多肽。 DNA分子可用于指定细胞中的基因产物，作为启动子或蛋白质编码序列或作为UTR或作为3'终止序列，并且还可用于控制染色体中基因的行为， 在控制基因的表达或用于遗传作图，识别或分离相同或相关的DNA片段，或特定个体生物体的鉴定或用于具有共同性状的一组生物体的聚类的工具。 具有该数据的本领域普通技术人员可以通过本领域已知的或本文描述的重组方法获得克隆的DNA片段，合成的DNA片段或构成所需序列的多肽。

公开(公告)号：US08362322B2

公开(公告)日：2013-01-29

申请号：US12446929

申请日：2007-10-26

申请人： Nestor Apuya ,  Steven Craig Bobzin ,  Jack Okamuro ,  Ke Zhang

发明人： Nestor Apuya ,  Steven Craig Bobzin ,  Jack Okamuro ,  Ke Zhang

IPC分类号： A01H5/00 ,  C12N15/63 ,  C12N15/82

CPC分类号： C12N15/8255 ,  C07K14/415

摘要： Materials and methods for modulating (e.g., increasing or decreasing) lignin content in plants are disclosed. For example, nucleic acids encoding lignin-modulating polypeptides are disclosed as well as methods for using such nucleic acids to generate transgenic plants having a modulated lignin content.

摘要翻译： 公开了调节（如增加或减少）植物中木质素含量的材料和方法。 例如，公开了编码木质素调节多肽的核酸以及使用此类核酸产生具有调节的木质素含量的转基因植物的方法。

公开(公告)号：US08124839B2

公开(公告)日：2012-02-28

申请号：US11916935

申请日：2006-06-08

申请人： Joon-Hyun Park ,  Nestor Apuya ,  Steven Craig Bobzin

发明人： Joon-Hyun Park ,  Nestor Apuya ,  Steven Craig Bobzin

IPC分类号： C12N15/29 ,  C12N15/82

CPC分类号： G01N33/5097

摘要： Materials and methods for identifying terpenoid regulatory region-regulatory protein associations are disclosed. Materials and methods for modulating expression of a sequence interest are also disclosed.

摘要翻译： 公开了鉴定萜类调节区调控蛋白关联的材料和方法。 还公开了调节序列感兴趣表达的材料和方法。

公开(公告)号：US20110041208A1

公开(公告)日：2011-02-17

申请号：US12891689

申请日：2010-09-27

申请人： Roger PENNELL ,  Kenneth A. Feldmann ,  Yu-Ping Lu ,  Yiwen Fang ,  Shing Kwok ,  Van-Dinh Dang ,  Zhihong Cook ,  Jack Okamuro ,  Nestor Apuya ,  Richard Schneeberger ,  Emilio Margolles-Clark ,  Edward A. Kiegle ,  Leonard Medrano

发明人： Roger PENNELL ,  Kenneth A. Feldmann ,  Yu-Ping Lu ,  Yiwen Fang ,  Shing Kwok ,  Van-Dinh Dang ,  Zhihong Cook ,  Jack Okamuro ,  Nestor Apuya ,  Richard Schneeberger ,  Emilio Margolles-Clark ,  Edward A. Kiegle ,  Leonard Medrano

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  A01H5/00

CPC分类号： C12N15/8216 ,  C12N15/8225 ,  C12N15/8226 ,  C12N15/8227 ,  C12N15/8229 ,  C12N15/823 ,  C12N15/8233 ,  C12N15/8234 ,  C12N15/8237

摘要： The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.

公开(公告)号：US20110016587A1

公开(公告)日：2011-01-20

申请号：US12698945

申请日：2010-02-02

申请人： Zhihong Cook ,  Yiwen Fang ,  Kenneth Feldmann ,  Edward A. Kiegle ,  Shing Kwok ,  Yu-Ping Lu ,  Leonard Medrano ,  Roger Pennell ,  Richard Schneeberger ,  Chuan-Yin Wu ,  Nestor Apuya ,  Diane Jofuku ,  Jonathan Donson

发明人： Zhihong Cook ,  Yiwen Fang ,  Kenneth Feldmann ,  Edward A. Kiegle ,  Shing Kwok ,  Yu-Ping Lu ,  Leonard Medrano ,  Roger Pennell ,  Richard Schneeberger ,  Chuan-Yin Wu ,  Nestor Apuya ,  Diane Jofuku ,  Jonathan Donson

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10

CPC分类号： C12N15/8216

摘要： The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.