公开(公告)号：US20180340169A1

公开(公告)日：2018-11-29

申请号：US15842550

申请日：2017-12-14

Applicant: 10X Genomics, Inc.

Inventor： Kamila Belhocine ,  Geoffrey McDermott ,  Francesca Meschi ,  Xinying Zheng

IPC: C12N15/10

Abstract: Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.

公开(公告)号：US20180312822A1

公开(公告)日：2018-11-01

申请号：US15975516

申请日：2018-05-09

Applicant: 10X Genomics, Inc.

Inventor： Josephine Lee ,  Samuel Marrs ,  Geoffrey McDermott ,  Francesca Meschi ,  Luz Montesclaros ,  Katherine Pfeiffer ,  Joseph Francis Shuga ,  Jessica Michele Terry ,  Solongo B. Ziraldo

IPC: C12N9/12 ,  C12N15/10 ,  C12Q1/6806

Abstract: Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition, greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes.