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44 条记录 (耗时 0.059 秒)

    High throughput screening for sequences of interest
    34.
    发明申请
    High throughput screening for sequences of interest 审中-公开
    感兴趣的序列的高通量筛选

    公开(公告)号：US20050064498A1

    公开(公告)日：2005-03-24

    申请号：US10960137

    申请日：2004-10-07

    申请人： Jay Short ,  Martin Keller

    发明人： Jay Short ,  Martin Keller

    IPC分类号： C12N1/12 ,  C12N1/20 ,  C12Q1/68 ,  G01N33/554 ,  G01N33/569

    CPC分类号： C12N15/1034 ,  C12N1/20 ,  C12N11/04 ,  C12N15/1086 ,  C12Q1/04 ,  C40B40/08 ,  C40B50/06 ,  G01N33/56911

    摘要： Provided is a method of screening or enriching a sample containing polynucleotides from a mixed population of organisms. The method includes creating a DNA library from a plurality of nucleic acid sequences of a mixed population of organisms and separating clones containing a polynucleotide sequence of interest on a fluorescence analyzer based upon hybridization of a fluorescence labeled oligonucleotide probe to a target sequence in a clone. The separated or enrich library can then be further process by activity based screening or sequence based screening. In addition, the enriched sequence can be compared to a database and to identify sequences in the database which have homology to a clone in the library thereby obtaining a nucleic acid profile of the mixed population of organisms.

    摘要翻译： 提供了筛选或富集来自生物体的混合群体的含有多核苷酸的样品的方法。 该方法包括基于荧光标记的寡核苷酸探针与克隆中的靶序列的杂交，在荧光分析仪上从多个生物体的多个核酸序列产生DNA文库和分离含有多核苷酸序列的克隆。 然后可以通过基于活性的筛选或基于序列的筛选进一步处理分离或富集的文库。 此外，可将富集的序列与数据库进行比较，并鉴定数据库中与文库中的克隆具有同源性的序列，从而获得混合生物群体的核酸谱。

    Production of enzymes having desired activities by mutagenesis
    35.
    发明申请
    Production of enzymes having desired activities by mutagenesis 审中-公开
    通过诱变生产具有所需活性的酶

    公开(公告)号：US20050009080A1

    公开(公告)日：2005-01-13

    申请号：US10912465

    申请日：2004-08-04

    申请人： Jay Short

    发明人： Jay Short

    IPC分类号： C12N9/00 ,  C12N9/16 ,  C12N9/24 ,  C12N15/10 ,  C12Q1/68 ,  C12N9/26

    CPC分类号： C12N9/00 ,  C12N9/16 ,  C12N9/2445 ,  C12N15/102 ,  C12N15/1034 ,  C12Y301/03001 ,  C12Y302/01021

    摘要： Disclosed is a process for obtaining an enzyme having a specified enzyme activity derived from a heterogeneous DNA population by screening, for the specified enzyme activity, a library of clones containing DNA from the heterogeneous DNA population which have been exposed to directed mutagenesis towards production of the specified enzyme activity. Also disclosed is a process for obtaining an enzyme having a specified enzyme activity by screening, for the specified enzyme activity, a library of clones containing DNA from a pool of DNA populations which have been exposed to directed mutagenesis in an attempt to produce in the library of clones DNA encoding an enzyme having one or more desired characteristics which can be the same or different from the specified enzyme activity.

    摘要翻译： 公开了一种获得具有来自异质DNA群体的特定酶活性的酶的方法，对于规定的酶活性，筛选含有已经暴露于定向诱变的异源DNA群体的DNA的克隆的文库， 指定的酶活性。 还公开了获得具有特定酶活性的酶的方法，通过筛选已经暴露于定向诱变的DNA群体中含有DNA的克隆的特定酶活性文库，试图在文库中产生 的克隆DNA，其编码具有一个或多个可以与指定酶活性相同或不同的所需特征的酶。

    Construction and use of catalogued nucleic acid libraries that contain advantageously adjusted representations of defined components
    38.
    发明申请
    Construction and use of catalogued nucleic acid libraries that contain advantageously adjusted representations of defined components 审中-公开

    公开(公告)号：US20060088821A1

    公开(公告)日：2006-04-27

    申请号：US10034622

    申请日：2001-12-21

    申请人： Jay Short

    发明人： Jay Short

    IPC分类号： C40B40/08 ,  C12Q1/68

    CPC分类号： C07H21/00 ,  C12N15/1093 ,  C40B40/00 ,  C40B40/08

    摘要： A process for constructing a catalogued nucleic acid library in which the proportional representation of the constituents is adjusted to advantage through the use of disclosed technologies for positive and negative selection. The resultant benefit is that significantly fewer library constituents will need to be screened in order to identify a potentially desired constituent. Moreover, library constituents that previously would have been essentially “lost” are now recoverable. Preferred embodiments of this invention include the cataloguing, normalization, and enrichment of library constituents. By way of example, but not limitation, this technology is serviceable for constructing a library that contains an adequate representation of desirable constituents that (1) are initially found in low-copy numbers within a sample source or (2) originate from an organism that is problematic to culture. Applicable uses of this invention include any library-screening endeavor previously hindered by logistical impediments. By expanding previous logistical frontiers this invention allows for a novel generation of previously unattainable molecules—particularly molecules that are “unclonable” from conventional, unadjusted libraries—to now be detected, cloned, manipulated, expressed, studied, and used. By disclosing the construction and screening of high yielding nucleic acid libraries from mixed and uncultivated organisms, the instant technology eclipses former boundaries in the area of biological discovery and enables the full breadth of biological diversity to be accessed in the search for previously undiscovered genes and gene products. The benefits of the present invention are seen to extend to areas of diagnosis, medicine, agriculture, manufacturing, and academia.

    Method of DNA shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process
    39.
    发明申请
    Method of DNA shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process 审中-公开
    通过阻断或中断合成或扩增过程产生的多核苷酸进行DNA改组的方法

    公开(公告)号：US20050208515A1

    公开(公告)日：2005-09-22

    申请号：US10981044

    申请日：2004-11-04

    申请人： Jay Short

    发明人： Jay Short

    IPC分类号： C12N15/10 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6844

    CPC分类号： C12N15/1027 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q2600/156 ,  C12Q2525/179 ,  C12Q2523/313

    摘要： Disclosed is a process of performing “sexual” PCR which includes generating random polynucleotides by interrupting or blocking a synthesis or amplification process to show or halt synthesis or amplification of at least one polynucleotide, optionally amplifying the polynucleotides, and reannealing the polynucleotides to produce random mutant polynucleotides. Also provided are vector and expression vehicles including such mutant polynucleotides, polypeptides expressed by the mutant polynucleotides and a method for producing random mutant polypeptides.

    摘要翻译： 披露了进行“性”PCR的过程，其包括通过中断或阻断合成或扩增过程产生随机多核苷酸，以显示或停止至少一种多核苷酸的合成或扩增，任选地扩增多核苷酸，以及重新退火多核苷酸以产生随机突变体 多核苷酸。 还提供了载体和表达载体，包括这种突变多核苷酸，由突变多核苷酸表达的多肽和用于产生随机突变多肽的方法。