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53 条记录 (耗时 0.033 秒)

    Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine
    33.
    发明授权
    Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 失效
    棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法

    公开(公告)号：US06908754B2

    公开(公告)日：2005-06-21

    申请号：US10284334

    申请日：2002-10-31

    申请人： Yoko Kuwabara ,  Kenichi Hashiguchi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yukiko Mori ,  Hisao Ito

    发明人： Yoko Kuwabara ,  Kenichi Hashiguchi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yukiko Mori ,  Hisao Ito

    IPC分类号： C12N1/21 ,  C12N9/00 ,  C12P13/10 ,  C12N9/10 ,  C07K17/00 ,  C12N9/12

    CPC分类号： C12N9/93 ,  C12P13/10

    摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.

    摘要翻译： 编码以下（a）或（b）中定义的多肽的DNA片段和以下（c）或（d）中定义的多肽：（a）至少具有氨基酸的氨基酸序列的多肽 序列表所示的SEQ ID NO：2中的50〜393的酸值，（b）至少具有序列表所示的序列号2所示的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代， 一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰磷酸合成酶活性的蛋白质，其具有氨基酸序列SEQ ID NO：3的氨基甲酰磷酸合成酶的大亚基，（c ）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，（d）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，包括取代，缺失，插入，添加， 或反转一个或几个氨基酸，并可构成 具有氨基酸 - 磷酸合成酶活性的蛋白质，其具有SEQ ID NO：2中氨基酸序列号为50〜393的氨基酸序列的氨基甲酰磷酸合成酶的小亚基。

    Dna encoding sucrose pts enzyme II
    34.
    发明授权
    Dna encoding sucrose pts enzyme II 失效
    Dna编码蔗糖酶Ⅱ

    公开(公告)号：US06893852B1

    公开(公告)日：2005-05-17

    申请号：US10019284

    申请日：2000-06-30

    申请人： Masako Izui ,  Masakazu Sugimoto ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

    发明人： Masako Izui ,  Masakazu Sugimoto ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

    IPC分类号： C12N9/12 ,  C12N15/54 ,  C07H21/04

    CPC分类号： C12N9/1205 ,  C12Y207/01069

    摘要： A gene encoding a protein constituting sucrose PTS of coryneform bacterium is provided by amplifying a region existing downstream from sucrase gene in coryneform bacterium by the cassette-ligation mediated PCR to obtain DNA encoding sucrose PTS enzyme II, which is a protein defined in the following (A) or (B): (A) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing; (B) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing including substitution, deletion, insertion, addition or inversion of one or several amino acids, and an activity for binding to sucrose.

    摘要翻译： 通过盒式连接介导的PCR扩增棒状杆菌型细菌的蔗糖酶基因下游的区域，得到编码构成棒状细菌的蔗糖PTS的蛋白质的基因，得到编码下述定义的蛋白质的蔗糖PTS酶II的DNA（ A）或（B）：（A）序列表中具有SEQ ID NO：2的氨基酸序列的蛋白质; （B）序列表中具有SEQ ID NO：2的氨基酸序列的蛋白质，包括一个或几个氨基酸的取代，缺失，插入，添加或转化，以及与蔗糖结合的活性。

    Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine
    35.
    发明授权
    Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权
    棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法

    公开(公告)号：US06255086B1

    公开(公告)日：2001-07-03

    申请号：US09629616

    申请日：2000-07-31

    申请人： Yoko Kuwabara ,  Kenichi Hashiguchi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yukiko Mori ,  Hisao Ito

    发明人： Yoko Kuwabara ,  Kenichi Hashiguchi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yukiko Mori ,  Hisao Ito

    IPC分类号： C12P1310

    CPC分类号： C12P13/10 ,  C12N9/93

    摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID No: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.

    摘要翻译： 编码以下（a）或（b）中定义的多肽的DNA片段，以及以下（c）或（d）:( a）定义的多肽，其至少具有氨基酸的氨基酸序列 序列表所示的SEQ ID NO：2中的酸值为50〜393，（b）至少具有序列表所示的SEQ ID No：2的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代， 一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰基 - 磷酸合成酶活性的蛋白质，其具有氨基酸 - 磷酸合成酶的大亚基，其氨基酸序列至少包含氨基酸数55至 SEQ ID NO：3的1113，（c）具有至少包含序列表所示的SEQ ID NO：3的氨基酸编号55至1113的氨基酸序列的多肽，（d）具有氨基酸的多肽 序列至少包含氨基酸序号55至1113 o f序列号3所示，包括一个或多个氨基酸的取代，缺失，插入，添加或倒位，并且可以构成具有氨基甲酰磷酸合成酶活性的氨基甲酰磷酸合成酶的小亚基的蛋白质， SEQ ID NO：2中氨基酸序列号为50〜393的氨基酸序列。

    Method for producing L-methionine by fermentation
    38.
    发明授权
    Method for producing L-methionine by fermentation 有权
    通过发酵生产L-甲硫氨酸的方法

    公开(公告)号：US07611873B1

    公开(公告)日：2009-11-03

    申请号：US09441055

    申请日：1999-11-16

    申请人： Yoshihiro Usuda ,  Osamu Kurahashi

    发明人： Yoshihiro Usuda ,  Osamu Kurahashi

    IPC分类号： C12P13/12 ,  C12P13/04 ,  C12N9/00 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12P13/12 ,  C12N9/0006 ,  C12N9/1018 ,  C12N9/1085 ,  C12N9/1205

    摘要： L-Methionine is produced by culturing a microorganism which is deficient in repressor of L-methionine biosynthesis system and/or enhanced intracellular homoserine transsuccinylase activity is cultured in a medium so that L-methionine should be produced and accumulated in the medium, and collecting the L-methionine from the medium. The microorganism preferably further exhibits reduced intracellular S-adenosylmethionine synthetase activity, L-threonine auxotrophy, enhanced intracellular cystathionine γ-synthase activity and enhanced intracellular aspartokinase-homoserine dehydrogenase II activity. The present invention enables breeding of L-methionine-producing bacteria, and L-methionine production by fermentation.

    摘要翻译： L-蛋氨酸通过培养在L-蛋氨酸生物合成系统的阻遏物不足的微生物和/或增强的细胞内高丝氨酸转氨基琥珀酰基酶活性在培养基中培养而产生，从而L-培养基中产生L-蛋氨酸并将其收集在培养基中 L-蛋氨酸来自培养基。 微生物优选进一步表现出细胞内S-腺苷甲硫氨酸合成酶活性降低，L-苏氨酸营养缺陷型，增强的细胞内胱硫醚γ-合成酶活性和增强的细胞内天冬氨酸激酶 - 高丝氨酸脱氢酶II活性。 本发明能够生产L-甲硫氨酸生产菌和通过发酵生产L-甲硫氨酸。

    METHOD FOR PRODUCING PURINE NUCLEOSIDE BY FERMENTATION
    39.
    发明申请
    METHOD FOR PRODUCING PURINE NUCLEOSIDE BY FERMENTATION 审中-公开
    通过发酵生产磷酸核苷的方法

    公开(公告)号：US20070161090A1

    公开(公告)日：2007-07-12

    申请号：US11682103

    申请日：2007-03-05

    申请人： Hiroshi MATSUI ,  Hisashi Kawasaki ,  Megumi Shimaoka ,  Yasuhiro Takenaka ,  Osamu Kurahashi

    发明人： Hiroshi MATSUI ,  Hisashi Kawasaki ,  Megumi Shimaoka ,  Yasuhiro Takenaka ,  Osamu Kurahashi

    IPC分类号： C12P19/28 ,  C12N1/21

    CPC分类号： C12N9/1077 ,  C12P19/38 ,  C12P19/40 ,  C12R1/19

    摘要： A microorganism which has a gene encoding an enzyme in which feedback inhibition is desensitized by substitution of one or two amino acids in PRPP amidotransferase encoded by purF of Escherichia coli, a gene encoding a protein which is an inactivated repressor of purine nucleotide biosynthesis encoded by purR, a gene encoding an enzyme which is inactivated purine nucleoside phosphorylase encoded by deoD, a gene encoding an enzyme which is inactivated succinyl-AMP synthase encoded by purA, a gene encoding an enzyme which is inactivated 6-phosphogluconate dehydrase encoded by edd, a gene encoding an enzyme which is inactivated phosphoglucose isomerase encoded by pgi and like is bred and a purine nucleoside is produced by culturing the microorganism.

    摘要翻译： 具有编码酶的基因的微生物，其中通过用大肠杆菌的purF编码的PRPP酰胺转移酶中的一个或两个氨基酸取代反馈抑制而脱敏，该基因是编码由purR编码的嘌呤核苷酸生物合成的灭活性阻遏物的蛋白质 编码由deoD编码的失活的嘌呤核苷磷酸化酶的酶的基因，编码由purA编码的失活的琥珀酰-PAMP合酶的酶的基因，编码由edd编码的6-磷酸葡萄糖酸脱水酶的酶的基因， 编码由pgi等编码的失活的磷酸葡萄糖异构酶的酶，并通过培养微生物产生嘌呤核苷。