摘要:
A Service Control Point (SCP) has a call list associated with the main telephone number of a called party. When a calling party attempts to contact the called party by calling the main number, but cannot reach the main number, a Service Switching Point (SSP) fires a trigger. One trigger is a T_No_Answer trigger, which is fired in response to a call to the main number that is not answered within a predefined number of rings. Another trigger is a T_Busy trigger that is fired in response to a call to the main number that is made when the main number is busy. Each trigger causes the SCP to access the call list and to cause the SSP to ring an alternative telephone number associated with the main telephone number. If this attempt to reach the called party also fails, then the SSP again fires the trigger and an attempt is made to connect the calling party to the next number on the call list. If the call list is exhausted before the calling party is connected to a number on the called party's call list, then a message can be played to the calling party. Alternatively, the calling party may be connected to a voice mailbox associated with the called party or may receive a busy tone.
摘要:
The invention provides assays for detecting the presence of the maize DAS-59132 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要:
This invention relates to plant breeding and the protection of plants from insects. More specifically, this invention includes novel transformation events of cotton plants comprising one or more polynucleotide sequences, as described herein, inserted into specific site(s) within the genome of a cotton cell. In highly preferred embodiments, said polynucleotide sequences encode “stacked” Cry1F and Cry1Ac lepidopteran insect inhibitory proteins. However, the subject invention includes plants having single cry1F or cry1Ac events, as described herein. Additionally, the invention is related to cotton plants derived from that transformation event and to assays for detecting the presence of the event in a sample. More specifically, the present invention provides DNA and related assays for detecting the presence of certain insect-resistance events in cotton. The assays are based on the DNA sequences of recombinant constructs inserted into the cotton genome and of the genomic sequences flanking the insertion sites. These sequences are unique. Based on these insert and border sequences, event-specific primers were generated. PCR analysis demonstrated that these cotton lines can be identified in different cotton genotypes by analysis of the PCR amplicons generated with these event-specific primer sets. Thus, these and other related procedures can be used to uniquely identify these cotton lines. Kits and conditions useful in conducting the assays are also provided. These materials and methods can also be used to assist breeding programs to further develop traits in cotton.
摘要:
The present invention provides methods and compositions for recombinant protein production through replication-defective adenoviral vector infection of non-trans-complementation cell lines. Thus, this invention describes methods of heterologous protein production without an accompanied production of adenoviral vectors.
摘要:
A Service Control Point (SCP) has a call list associated with the main telephone number of a called party. When a calling party attempts to contact the called party by calling the main number, but cannot reach the main number, a Service Switching Point (SSP) fires a trigger. One trigger is a T_No_Answer trigger, which is fired in response to a call to the main number that is not answered within a predefined number of rings. Another trigger is a T_Busy trigger that is fired in response to a call to the main number that is made when the main number is busy. Each trigger causes the SCP to access the call list and to cause the SSP to ring an alternative telephone number associated with the main telephone number. If this attempt to reach the called party also fails, then the SSP again fires the trigger and an attempt is made to connect the calling party to the next number on the call list. If the call list is exhausted before the calling party is connected to a number on the called party's call list, then a message can be played to the calling party. Alternatively, the calling party may be connected to a voice mailbox associated with the called party or may receive a busy tone.
摘要:
This invention relates to plant breeding and the protection of plants from insects. More specifically, this invention includes novel transformation events of cotton plants comprising one or more polynucleotide sequences, as described herein, inserted into specific site(s) within the genome of a cotton cell. In highly preferred embodiments, said polynucleotide sequences encode “stacked” Cry1F and Cry1Ac lepidopteran insect inhibitory proteins. However, the subject invention includes plants having single cry1F or cry1Ac events, as described herein. Additionally, the invention is related to cotton plants derived from that transformation event and to assays for detecting the presence of the event in a sample. More specifically, the present invention provides DNA and related assays for detecting the presence of certain insect-resistance events in cotton. The assays are based on the DNA sequences of recombinant constructs inserted into the cotton genome and of the genomic sequences flanking the insertion sites. These sequences are unique. Based on these insert and border sequences, event-specific primers were generated. PCR analysis demonstrated that these cotton lines can be identified in different cotton genotypes by analysis of the PCR amplicons generated with these event-specific primer sets. Thus, these and other related procedures can be used to uniquely identify these cotton lines. Kits and conditions useful in conducting the assays are also provided. These materials and methods can also be used to assist breeding programs to further develop traits in cotton.
摘要:
This invention relates to plant breeding and the protection of plants from insects. More specifically, this invention includes novel transformation events of cotton plants comprising one or more polynucleotide sequences, as described herein, inserted into specific site(s) within the genome of a cotton cell. In highly preferred embodiments, said polynucleotide sequences encode “stacked” Cry1F and Cry1Ac lepidopteran insect inhibitory proteins. However, the subject invention includes plants having single cry1F or cry1Ac events, as described herein. Additionally, the invention is related to cotton plants derived from that transformation event and to assays for detecting the presence of the event in a sample. More specifically, the present invention provides DNA and related assays for detecting the presence of certain insect-resistance events in cotton. The assays are based on the DNA sequences of recombinant constructs inserted into the cotton genome and of the genomic sequences flanking the insertion sites. These sequences are unique. Based on these insert and border sequences, event-specific primers were generated. PCR analysis demonstrated that these cotton lines can be identified in different cotton genotypes by analysis of the PCR amplicons generated with these event-specific primer sets. Thus, these and other related procedures can be used to uniquely identify these cotton lines. Kits and conditions useful in conducting the assays are also provided. These materials and methods can also be used to assist breeding programs to further develop traits in cotton.