Methods of mapping polymorphisms and polymorphism microarrays
    38.
    发明授权
    Methods of mapping polymorphisms and polymorphism microarrays 有权
    多态性和多态性微阵列的绘制方法

    公开(公告)号:US09365893B2

    公开(公告)日:2016-06-14

    申请号:US11914099

    申请日:2006-05-10

    IPC分类号: C12Q1/68 C40B20/04 C40B30/04

    摘要: Described are methods for the high-throughput discovery and genotyping of nucleotide polymorphisms in DNA, including single nucleotide polymorphism (SNPs) and short deletions and insertions. These methods take advantage of the fact that differences in DNA sequence result in the differential presence of restriction endonuclease digestion sites. Approaches involve isolation of short DNA fragments (“tags”) near restriction endonuclease sites. The presence of one (or two) of these tags indicates that a site was present. Regions of DNA with a restriction site in only one individual create an opportunity for primer extension to produce labeled material, which can be assayed on a platform that employs a collection of nucleic acids. Efficient variant detection microarrays and bead libraries are provided that contain genomic tags with different representations between two populations, so that most elements in the collection of nucleic acids contain a SNP between populations of interest.

    摘要翻译: 描述了DNA的核苷酸多态性的高通量发现和基因分型的方法,包括单核苷酸多态性(SNPs)和短缺失和插入。 这些方法利用DNA序列的差异导致限制性内切核酸酶消化位点的差异存在的事实。 方法包括在限制性内切核酸酶位点附近分离短DNA片段(“标签”)。 一个(或两个)这些标签的存在表明存在一个站点。 仅在一个个体中具有限制性位点的DNA区域产生引物延伸以产生标记物质的机会,其可以在采用核酸集合的平台上测定。 提供了有效的变体检测微阵列和珠文库,其包含两个群体之间具有不同表示的基因组标签,使得核酸收集中的大多数元素在感兴趣的群体之间含有SNP。

    Apparatus for separating melon flesh from rind
    39.
    发明授权
    Apparatus for separating melon flesh from rind 有权
    将瓜肉与皮分离的装置

    公开(公告)号:US09016195B1

    公开(公告)日:2015-04-28

    申请号:US11469168

    申请日:2006-08-31

    IPC分类号: A23B5/00 A23N7/08

    摘要: An apparatus for preparing a melon juice concentrate, such as watermelon juice concentrate, includes a finisher with brushes for separating melon flesh from melon rind including leaving at least 1/16 inch flesh on the rind and screens for separating juice from flesh. The apparatus also includes a steamer to reduce bacteria count on whole melon, a chopper for chopping melons into pieces less than about 16 inches square, an extractor for extracting juice from the flesh, and an evaporator for concentrating melon juice to form melon juice concentrate. The steamer includes a cylinder frame, steam jets directing steam through apertures in the cylinder frame at whole melon therein, and a motivator to move the whole melon along the cylinder frame. The apparatus can also process cantaloupe, honeydew melon, and other melon.

    摘要翻译: 用于制备甜瓜汁浓缩物如西瓜汁浓缩物的装置包括具有用于从瓜皮分离瓜肉的刷子的整理器,包括在皮肉上留下至少1/16英寸的肉和用于从果肉分离果汁的筛网。 该设备还包括蒸笼以减少整个瓜上的细菌数量,将瓜切成小于约16英寸平方的碎片的切碎机,用于从肉中提取果汁的提取器和用于浓缩瓜汁以形成瓜汁浓缩物的蒸发器。 蒸笼包括气缸框架,蒸汽喷嘴引导蒸汽通过气缸框架中的整个甜瓜中的孔,以及促动器沿着气缸框架移动整个甜瓜。 该设备还可以处理哈密瓜,蜜瓜和其他甜瓜。