公开(公告)号：US20160289628A1

公开(公告)日：2016-10-06

申请号：US15142913

申请日：2016-04-29

申请人： Heliae Development, LLC

发明人： Luke Cizek ,  Miquel Olaizola ,  Mason McCarty ,  Mason Oelschlager

IPC分类号： C12N1/12

CPC分类号： C12N1/12 ,  C12M21/02 ,  C12M23/14 ,  C12M23/26 ,  C12M23/48 ,  C12M27/04 ,  C12M29/04 ,  C12M37/00 ,  C12M41/22 ,  C12M41/36 ,  C12M41/40

摘要： Some embodiments include a method of culturing one or more microalgae. The method can include: inoculating a bioreactor with the one or more microalgae and a fluidic support medium, the bioreactor having one or more bioreactor walls at least partially enclosing a bioreactor cavity, being configured to be at least one of folded up or rolled up, and being sterile when the bioreactor is inoculated with the one or more microalgae, the one or more bioreactor walls comprising at least one bioreactor wall material, and the at least one bioreactor wall material being flexible and at least partially transparent; and vitally supporting the one or more microalgae. Other embodiments of related systems and methods are also disclosed.

摘要翻译： 一些实施方案包括培养一种或多种微藻的方法。 该方法可以包括：用一种或多种微藻和流体支持介质接种生物反应器，所述生物反应器具有至少部分包围生物反应器腔的一个或多个生物反应器壁，其被配置为折叠或卷起至少一个， 并且当所述生物反应器接种所述一种或多种微藻时，所述一个或多个生物反应器壁包含至少一个生物反应器壁材料，并且所述至少一个生物反应器壁材料是柔性的并且至少部分透明; 并且极大地支持一种或多种微藻。 还公开了相关系统和方法的其它实施例。

公开(公告)号：US09447375B1

公开(公告)日：2016-09-20

申请号：US14796720

申请日：2015-07-10

申请人： HELIAE DEVELOPMENT LLC

发明人： Laura Carney ,  Kristine Sorensen

IPC分类号： C12N1/12 ,  C12Q1/02

CPC分类号： C12N1/12 ,  A01N59/08 ,  C12N1/38 ,  C12P23/00 ,  C12P33/00 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2333/405

摘要： Methods of treating lysis in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises dosing the culture comprising with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

摘要翻译： 本文描述了用过氧化氢处理流感嗜血杆菌的培养物中的裂解方法。 该方法包括在培养过程中基于细胞阶段的一定浓度的过氧化氢给予培养物，并且频率增加细胞存活的可能性，直到累积类胡萝卜素（如虾青素）的过程完成。

公开(公告)号：US09447373B1

公开(公告)日：2016-09-20

申请号：US14795675

申请日：2015-07-09

申请人： HELIAE DEVELOPMENT LLC

发明人： Laura Carney ,  Kristine Sorensen

IPC分类号： C12N1/12 ,  C12Q1/06

CPC分类号： C12N1/12 ,  A01N59/08 ,  C12N1/38 ,  C12P23/00 ,  C12P33/00 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2333/405

摘要： Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide and salt are described herein. The method comprises dosing the culture comprising a concentration of salt with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

摘要翻译： 本文描述了用过氧化氢和盐处理红潮球红藻的培养物中的污染物，特别是真菌污染物的方法。 该方法包括在培养过程中基于细胞阶段，并以增加细胞存活的可能性的频率，将包含一定浓度的过氧化氢浓度的培养物给药，直到累积类胡萝卜素如虾青素的过程 ， 做完了。

公开(公告)号：US09416336B2

公开(公告)日：2016-08-16

申请号：US14877121

申请日：2015-10-07

申请人： HELIAE DEVELOPMENT LLC

发明人： Sandip Shinde

IPC分类号： C11C1/00 ,  C11C1/10 ,  C11C1/08 ,  C11B3/04 ,  C11B3/12 ,  C11C3/10

CPC分类号： C11C1/10 ,  C11B3/04 ,  C11B3/12 ,  C11C1/005 ,  C11C1/007 ,  C11C1/08 ,  C11C3/10

摘要： Methods of producing fatty acid ethyl esters (FAEE) using a direct transesterification process are described. The direct transesterification process uses low levels of chemical solvents, acid catalysts, and heating energy to produce the FAEE in a method with increased efficiency in a co-solvent system. The FAEE produced may be used in a variety of products including health, beauty, nutraceutical, and cosmetic products.

公开(公告)号：US09113607B1

公开(公告)日：2015-08-25

申请号：US14667917

申请日：2015-03-25

申请人： Heliae Development, LLC

发明人： Laura T. Carney ,  Kristine Sorensen

IPC分类号： C12N1/12 ,  A01G33/00

CPC分类号： C12N1/12 ,  A01N59/08 ,  C12N1/38 ,  C12P23/00 ,  C12P33/00 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2333/405

摘要： Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises dosing the culture with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

摘要翻译： 本文描述了用过氧化氢处理冲积红球菌的培养物中污染物，特别是真菌污染物的方法。 该方法包括在培养过程中基于细胞的阶段向培养物中加入浓度为过氧化氢的培养物，并且频率增加细胞存活的可能性，直到累积类胡萝卜素（例如虾青素）的过程完成。

公开(公告)号：US08709258B2

公开(公告)日：2014-04-29

申请号：US13899813

申请日：2013-05-22

申请人： Heliae Development, LLC

发明人： Justin S. Kniep ,  Aniket Kale

IPC分类号： C02F1/30 ,  C02F1/46

CPC分类号： C12N13/00 ,  B03D2203/003 ,  C12M47/02 ,  C12N1/02 ,  C12N1/12

摘要： Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may comprise different types of pulses. In some embodiments, the flow path for the aqueous suspension may vary.

摘要翻译： 本文描述了用于在水性悬浮液中聚集微生物的系统，方法和装置。 特别地，当水悬浮液遵循流动路径以引起微生物聚集时，将电场施加到包含微生物的水性悬浮液中的系统，方法和装置。 电场可以包括不同类型的脉冲。 在一些实施方案中，水性悬浮液的流动路径可以变化。

公开(公告)号：US08702991B2

公开(公告)日：2014-04-22

申请号：US13899769

申请日：2013-05-22

申请人： Heliae Development, LLC

发明人： Justin S. Kniep ,  Aniket Kale

IPC分类号： C02F1/463

CPC分类号： C12N13/00 ,  B03D2203/003 ,  C12M47/02 ,  C12N1/02

摘要： Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary.

摘要翻译： 本文描述了用于在水性悬浮液中聚集微生物的系统，方法和装置。 特别地，当水悬浮液遵循流动路径以引起微生物聚集时，将电场施加到包含微生物的水性悬浮液中的系统，方法和装置。 电场可以是连续的或脉冲的。 在一些实施方案中，水性悬浮液的流动路径可以变化。

公开(公告)号：US20140017754A1

公开(公告)日：2014-01-16

申请号：US13733217

申请日：2013-01-03

申请人： HELIAE DEVELOPMENT, LLC

发明人： Aniket KALE ,  Justin S. KNIEP

IPC分类号： C12N13/00

CPC分类号： C12N13/00 ,  C12M33/12 ,  C12M47/02

摘要： Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field and/or acoustic energy to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary. In some embodiments, the cross-sectional area of the electrical field may be tuned.

摘要翻译： 本文描述了用于在水性悬浮液中聚集微生物的系统，方法和装置。 特别地，是当包含微生物的水性悬浮液伴随着流动路径引起微生物聚集时，将电场和/或声能施加到水性悬浮液的系统，方法和装置。 电场可以是连续的或脉冲的。 在一些实施方案中，水性悬浮液的流动路径可以变化。 在一些实施例中，可以调整电场的横截面积。

公开(公告)号：US08569531B2

公开(公告)日：2013-10-29

申请号：US13530937

申请日：2012-06-22

申请人： Aniket Kale

发明人： Aniket Kale

IPC分类号： C11B7/00

CPC分类号： C12P7/649 ,  C11B1/10 ,  C11B1/108 ,  C11C3/003 ,  C12N1/12 ,  C12P7/6427 ,  C12P7/6472 ,  C12P17/182 ,  C12P23/00 ,  Y02E50/13

摘要： A method for isolating chlorophylls from intact algal cells is provided. The method includes dewatering intact algal cells from an algal cell culture to make an algal biomass, extracting lipids along with carotenoids and chlorophylls from the algal biomass, and separation of the chlorophylls using adsorption or membrane diafiltration or other methods. The method can include selective extraction of other algal components including polar lipids, neutral lipids, proteins, and carbohydrates. The method may include esterifying the lipids with a catalyst in the presence of an alcohol, and separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising omega-3 fatty acids esters and remaining carotenoids.

摘要翻译： 提供了从完整藻细胞分离叶绿素的方法。 该方法包括从藻类细胞培养物中脱水完整的藻类细胞以制备藻类生物质，从藻类生物质中提取脂质以及类胡萝卜素和叶绿素，以及使用吸附或膜渗滤或其他方法分离叶绿素。 该方法可以包括选择性提取其他藻类成分，包括极性脂质，中性脂质，蛋白质和碳水化合物。 该方法可以包括在醇的存在下用催化剂酯化脂质，并从包含燃料酯的水不溶性部分中分离包含甘油的水溶性馏分，并在真空下蒸馏燃料酯以获得C16或更短的燃料酯馏分， C16或更长的燃料酯馏分，以及包含ω-3脂肪酸酯和剩余的类胡萝卜素的残基。

公开(公告)号：US20130217904A1

公开(公告)日：2013-08-22

申请号：US13848442

申请日：2013-03-21

申请人： Heliae Development, LLC

发明人： Aniket KALE

IPC分类号： C11B1/10

CPC分类号： C11B1/10 ,  B01D11/028 ,  B01D11/0288 ,  C10L2290/544 ,  C11B1/04 ,  C11B1/108

摘要： A method for separating polar lipids and neutral lipids from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting polar lipids and neutral lipids from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal polar lipids and neutral lipids from a wet algal biomass while avoiding emulsification of extraction mixtures. These polar lipids are high value products which can be used as surfactants, detergents, and food additives. These neutral lipids are also high value products which can be used to generate renewable fuels.

摘要翻译： 使用两亲性溶剂组和疏水性溶剂组从植物材料，特别是完整的藻类细胞中分离极性脂质和中性脂质的方法。 一些实施方案包括将完整的藻类细胞脱水，然后从藻类细胞中提取极性脂质和中性脂质。 该方法提供单步和多步提取方法，其允许藻类极性脂质和中性脂质从湿藻类生物质中有效分离，同时避免萃取混合物的乳化。 这些极性脂质是可用作表面活性剂，洗涤剂和食品添加剂的高价值产品。 这些中性脂质也是可用于生产可再生燃料的高价值产品。