公开(公告)号：US06891085B2

公开(公告)日：2005-05-10

申请号：US10125258

申请日：2002-04-18

申请人： Daniel J. Altier ,  Rafael Herrmann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  Janine L. Weaver ,  James F. H. Wong

发明人： Daniel J. Altier ,  Rafael Herrmann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  Janine L. Weaver ,  James F. H. Wong

IPC分类号： C07K14/435 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N7/01 ,  C12N15/12 ,  C12N15/82 ,  A10H5/00 ,  C12N15/11 ,  C12N15/63 ,  A10H5/10

CPC分类号： C12N15/8282 ,  C07K14/43563 ,  Y10S530/858

摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include nucleic acid molecules encoding Fus6 polypeptides derived from Agrotis ipsilon that possess antimicrobial properties, particularly fungicidal properties. Further provided are plant cells plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.

摘要翻译： 本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。 本发明的组合物包括编码具有抗微生物性质，特别是杀真菌性质的源自Agrotis ipsilon的Fus6多肽的核酸分子。 还提供了用本发明的核酸分子转化的植物细胞植物及其种子，以赋予植物抗病性。

公开(公告)号：US07462760B2

公开(公告)日：2008-12-09

申请号：US10746914

申请日：2003-12-24

申请人： André Abad ,  Hua Dong ,  Rafael Herrmann ,  Albert Lu ,  Billy F. McCutchen ,  Janet A. Rice ,  Eric J. Schepers ,  James F. Wong

发明人： André Abad ,  Hua Dong ,  Rafael Herrmann ,  Albert Lu ,  Billy F. McCutchen ,  Janet A. Rice ,  Eric J. Schepers ,  James F. Wong

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/32

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide expression cassettes and transformed plants, plant cells, and seeds. These compositions find use in methods for protecting a plant from a pest.

摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 本发明提供已被工程化以编码具有增加的抗植物蛋白酶对蛋白水解降解的杀虫多肽的诱变的核酸。 特别地，提供了编码杀虫多肽的核酸序列，其被修饰为包含赋予植物蛋白酶降解或蛋白水解灭活抗性的蛋白水解保护位点。 本发明的具体实施方案提供表达盒和转化植物，植物细胞和种子。 这些组合物用于保护植物免受害虫的方法。

公开(公告)号：US07396980B2

公开(公告)日：2008-07-08

申请号：US11123896

申请日：2005-05-06

申请人： Carl R. Simmons ,  Pedro A. Navarro Acevedo ,  Leslie Harvell ,  Rebecca Cahoon ,  Billy F. McCutchen ,  Albert L. Lu ,  Rafael Herrmann ,  James F. Wong

发明人： Carl R. Simmons ,  Pedro A. Navarro Acevedo ,  Leslie Harvell ,  Rebecca Cahoon ,  Billy F. McCutchen ,  Albert L. Lu ,  Rafael Herrmann ,  James F. Wong

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/29

CPC分类号： C07K14/4723 ,  C07K14/415 ,  C12N15/8279

摘要： Methods and compositions for modulating development and defense responses are provided. Nucleotide sequences encoding defensin proteins are provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and defense responses. Transformed plants, plant cells, tissues, and seed are also provided.

摘要翻译： 提供了调节发展和防御反应的方法和组成。 提供编码防御素蛋白质的核苷酸序列。 序列可用于表达盒用于调节发育，发育途径和防御反应。 还提供了转化的植物，植物细胞，组织和种子。

公开(公告)号：US08148077B2

公开(公告)日：2012-04-03

申请号：US11780884

申请日：2007-07-20

申请人： Andre R. Abad ,  Hua Dong ,  Susan B. Lo ,  Billy F. McCutchen ,  Xiaomei Shi

发明人： Andre R. Abad ,  Hua Dong ,  Susan B. Lo ,  Billy F. McCutchen ,  Xiaomei Shi

IPC分类号： C12P19/34

CPC分类号： C12N15/8286 ,  C12Q1/6813 ,  Y02A40/162 ,  C12Q2531/113

摘要： Methods and compositions for identifying novel genes that share regions of homology with known genes from target groups of genes of interest are provided. The methods comprise systematically designing oligonucleotide primers that are specific for regions of homology within the nucleotide sequences of a target group of known genes and performing successive rounds of PCR amplification of nucleic acid material from an organism of interest. The PCR steps are intended to identify and amplify nucleic acids comprising both known and novel genes. Nucleic acid molecules comprising known genes are detected and eliminated from further consideration by dot blot analysis using oligonucleotide probes specific for the known genes in the target group. Potentially novel genes are subjected to further sequence analysis to confirm novelty and assayed for biological activity. Compositions of the present invention include novel polynucleotides, and variants and fragments thereof, that comprise novel genes and the polypeptides encoded thereby.

摘要翻译： 提供了用于鉴定与目标基因组的已知基因具有同源性区域的新基因的方法和组合物。 所述方法包括系统地设计对已知基因的靶组的核苷酸序列内的同源区特异性的寡核苷酸引物，并且从感兴趣的生物体进行连续循环的核酸材料的PCR扩增。 PCR步骤旨在鉴定和扩增包含已知和新基因的核酸。 通过使用针对靶组中已知基因特异的寡核苷酸探针的斑点印迹分析，进一步考虑检测并消除包含已知基因的核酸分子。 将潜在的新基因进行进一步的序列分析以证实新颖性并测定生物活性。 本发明的组合物包括新型多核苷酸及其变体和片段，其包含新基因和由其编码的多肽。

公开(公告)号：US07790952B1

公开(公告)日：2010-09-07

申请号：US12124978

申请日：2008-05-21

申请人： Scott Diehn ,  Albert L. Lu ,  Billy F. McCutchen ,  Lynne E. Sims ,  Kim R. Ward

发明人： Scott Diehn ,  Albert L. Lu ,  Billy F. McCutchen ,  Lynne E. Sims ,  Kim R. Ward

IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N15/63 ,  A01H1/00

CPC分类号： C12N15/8239

摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for an inducible promoter for the gene encoding ZmPOX24. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码ZmPOX24的基因的诱导型启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：US07439348B2

公开(公告)日：2008-10-21

申请号：US11328870

申请日：2006-01-10

申请人： Scott Diehn ,  Albert L. Lu ,  Billy F. McCutchen ,  Lynne E. Sims ,  Kim R. Ward

发明人： Scott Diehn ,  Albert L. Lu ,  Billy F. McCutchen ,  Lynne E. Sims ,  Kim R. Ward

IPC分类号： C12N15/82 ,  C12N15/00 ,  C12N15/05 ,  C12N15/11 ,  C12N15/63 ,  C12N5/10 ,  C12N5/04 ,  A01H5/10

CPC分类号： C12N9/0004 ,  C12N15/8227 ,  C12N15/8237 ,  C12N15/8239

摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for an inducible promoter for the gene encoding a maize deoxyhypusine synthase. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码玉米脱氧酪氨酸合成酶的基因的诱导型启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：US07393947B1

公开(公告)日：2008-07-01

申请号：US11274626

申请日：2005-11-15

申请人： Scott Diehn ,  Albert L. Lu ,  Billy F. McCutchen ,  Lynne E. Sims ,  Kim R. Ward

发明人： Scott Diehn ,  Albert L. Lu ,  Billy F. McCutchen ,  Lynne E. Sims ,  Kim R. Ward

IPC分类号： C12N15/82 ,  C12N15/63

CPC分类号： C12N15/8239

摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for an inducible promoter for the gene encoding ZmPOX24. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码ZmPOX24的基因的诱导型启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：US07378499B2

公开(公告)日：2008-05-27

申请号：US11224624

申请日：2005-09-12

申请人： André Abad ,  Hua Dong ,  Rafael Herrmann ,  Albert Lu ,  Billy F. McCutchen ,  Janet Rice ,  Eric Schepers ,  James Wong

发明人： André Abad ,  Hua Dong ,  Rafael Herrmann ,  Albert Lu ,  Billy F. McCutchen ,  Janet Rice ,  Eric Schepers ,  James Wong

IPC分类号： C07K1/00

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide pesticidal polypeptide compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. These compositions find use in methods for controlling pests, especially plant pests. Novel plant proteases, sequences encoding these proteases, and methods for their use are also provided.

摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 本发明提供已被工程化以编码具有增加的抗植物蛋白酶对蛋白水解降解的杀虫多肽的诱变的核酸。 特别地，提供了编码杀虫多肽的核酸序列，其被修饰为包含赋予植物蛋白酶降解或蛋白水解灭活抗性的蛋白水解保护位点。 本发明的具体实施方案提供杀虫多肽组合物和制剂，表达盒和转化植物，植物细胞和种子。 这些组合物可用于防治害虫，特别是植物害虫的方法。 还提供了新型植物蛋白酶，编码这些蛋白酶的序列及其使用方法。

公开(公告)号：US06864403B1

公开(公告)日：2005-03-08

申请号：US09417251

申请日：1999-10-13

申请人： Rebecca E. Cahoon ,  Guo-Hua Miao ,  Rafael Herrmann ,  J. Antoni Rafalski ,  Billy F. McCutchen

发明人： Rebecca E. Cahoon ,  Guo-Hua Miao ,  Rafael Herrmann ,  J. Antoni Rafalski ,  Billy F. McCutchen

IPC分类号： C12N9/90 ,  A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12Q1/68 ,  G01N33/573

CPC分类号： C12N9/90 ,  G01N2333/99

摘要： This invention relates to an isolated nucleic acid fragment encoding a protein disulfide isomerase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the protein disulfide isomerase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the protein disulfide isomerase in a transformed host cell.

摘要翻译： 本发明涉及编码蛋白质二硫键异构酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分蛋白质二硫键异构酶的嵌合基因，其中嵌合基因的表达导致转化的宿主细胞中蛋白质二硫键异构酶水平的改变 。

公开(公告)号：US07193135B2

公开(公告)日：2007-03-20

申请号：US11299452

申请日：2005-12-12

申请人： Scott Diehn ,  Albert L. Lu ,  Billy F. McCutchen ,  Lynne E. Sims ,  Kim R. Ward

发明人： Scott Diehn ,  Albert L. Lu ,  Billy F. McCutchen ,  Lynne E. Sims ,  Kim R. Ward

IPC分类号： C12P7/62 ,  C12P11/00 ,  C07H21/04 ,  A01H1/00

CPC分类号： C12N9/0069 ,  C07K14/415 ,  C12N15/8227 ,  C12N15/8237 ,  C12N15/8239

摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for an inducible promoter for the gene encoding a maize 2-oxoglutarate-dependent oxygenase. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码玉米2-氧戊二酸依赖性加氧酶的基因的诱导型启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。