公开(公告)号：US06783699B2

公开(公告)日：2004-08-31

申请号：US10274253

申请日：2002-10-17

申请人： Guangjun Li ,  Hong Wu ,  Chunyang Liu

发明人： Guangjun Li ,  Hong Wu ,  Chunyang Liu

IPC分类号： C09K1102

CPC分类号： G01N21/6428 ,  C09K11/7792 ,  C12Q1/6816 ,  Y10T428/2991 ,  Y10T428/2995 ,  C12Q2563/155

摘要： Europium-containing fluorescent nanoparticles and methods of preparing such nanoparticles are provided. The nanoparticles are comprised of an aluminum oxide framework having a europium activator, a magnesium, calcium, strontium, or barium energy reservoir, and at least one co-activator selected from the group consisting of scandium, yttrium, and certain lanthanide elements. The nanoparticles may be optionally coated with a silane coating containing reactive functional groups that allow for attachment of the nanoparticle to a desired biological or chemical target molecule. The coated nanoparticles may be used to detect a variety of protein ligand interactions, and may also be used in assays for nucleic acids.

摘要翻译： 提供含铕荧光纳米颗粒及其制备方法。 纳米颗粒由具有铕活化剂，镁，钙，锶或钡能量储存器的氧化铝框架和至少一种选自钪，钇和某些镧系元素的共活化剂组成。 纳米颗粒可以任选地涂覆有含有反应性官能团的硅烷涂层，所述反应性官能团允许纳米颗粒附着到所需的生物或化学靶分子上。 包被的纳米颗粒可用于检测各种蛋白质配体相互作用，并且也可用于核酸测定。

公开(公告)号：US06692957B2

公开(公告)日：2004-02-17

申请号：US09836570

申请日：2001-04-17

申请人： Steven A. Goldman ,  Hong Wu

发明人： Steven A. Goldman ,  Hong Wu

IPC分类号： C12N502

CPC分类号： C12Q1/6897

摘要： The present invention is directed to a method of separating cells of interest which method comprises: determining cells of interest; selecting a promoter specific for the cells of interest; introducing a nucleic acid molecule encoding green fluorescent protein under control of the promoter into a plurality of cells; and separating cells of the plurality of cells that are expressing said green fluorescent protein, wherein the separated cells are the cells of interest.

摘要翻译： 本发明涉及一种分离感兴趣的细胞的方法，该方法包括：确定感兴趣的细胞; 选择对感兴趣的细胞特异性的启动子; 将编码绿色荧光蛋白的核酸分子引导至多个细胞; 以及分离表达所述绿色荧光蛋白的多个细胞的细胞，其中分离的细胞是感兴趣的细胞。