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公开(公告)号：US20200294784A1
公开(公告)日：2020-09-17
申请号：US16815811
申请日：2020-03-11
Applicant: Thermo Fisher Scientific (Bremen) GmbH
Inventor： Dirk Nolting , Alexander A. Makarov , Amelia Corinne Petersen
IPC: H01J49/42
Abstract: Trapping ions in an ion trapping assembly is described. In one aspect, this is implemented by introducing ions into the ion trapping assembly, applying a first RF trapping amplitude to the ion trapping assembly so as to trap introduced ions which have m/z ratios within a first range of m/z ratios, and cooling the trapped ions. In some aspects, also performed is reducing the RF trapping amplitude from the first RF trapping amplitude to a second, lower, RF trapping amplitude so as to reduce the low mass cut-off of the ion trapping assembly and trapping, at the second, lower RF trapping amplitude, introduced ions having m/z ratios within a second range of m/z ratios. A lower mass limit of the second range of m/z ratios is below the low mass cut-off of the ion trapping assembly when the first RF trapping amplitude is applied.
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公开(公告)号：US20200264142A1
公开(公告)日：2020-08-20
申请号：US16866737
申请日：2020-05-05
Applicant: Thermo Fisher Scientific (Bremen) GmbH
Inventor： Yue XUAN
Abstract: A data independent acquisition method of mass spectrometry for analysing a sample as it elutes from a chromatography system is disclosed. The method comprises selecting a precursor mass range, and performing a plurality of MS1 scans and sets of MS2 scans across the precursor mass range. Each of the MS1 scans uses a mass analyser operated at a first, relatively higher resolution, for identification and/or quantitation of the sample in the MS1 domain. The set of MS2 scans comprises performing MS2 scans of fragmented mass range segments performed with the mass analyser, operated at a second, relatively lower resolution. In the method, the MS1 scans are interleaved throughout the performing of the set of MS2 scans such that the MS1 scans provide a mass chromatogram of the sample. The ratio of the number of MS1 scans to sets of MS2 scans performed across the chromatographic peak width is at least 3:1.
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公开(公告)号：US20200243322A1
公开(公告)日：2020-07-30
申请号：US16697329
申请日：2019-11-27
Applicant: Thermo Fisher Scientific (Bremen) GmbH
Inventor： Hamish Stewart , Dmitry E. Grinfeld , Alexander A. Makarov
Abstract: A multi-reflection mass spectrometer comprising two ion mirrors spaced apart and opposing each other in a direction X, each mirror elongated generally along a drift direction Y, the drift direction Y being orthogonal to the direction X, a pulsed ion injector for injecting pulses of ions into the space between the ion mirrors, the ions entering the space at a non-zero inclination angle to the X direction, the ions thereby forming an ion beam that follows a zigzag ion path having N reflections between the ion mirrors in the direction X whilst drifting along the drift direction Y, a detector for detecting ions after completing the same number N of reflections between the ion mirrors, and an ion focusing arrangement at least partly located between the opposing ion mirrors and configured to provide focusing of the ion beam in the drift direction Y, such that a spatial spread of the ion beam in the drift direction Y passes through a single minimum at or immediately after a reflection having a number between 0.25N and 0.75N, wherein all detected ions are detected after completing the same number N of reflections between the ion mirrors.
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公开(公告)号：US10714322B2
公开(公告)日：2020-07-14
申请号：US16575312
申请日：2019-09-18
Applicant: Thermo Fisher Scientific (Bremen) GmbH
Inventor： Alexander A. Makarov , Stevan R. Horning
Abstract: A sample introduction system for a spectrometer comprises a desolvation region that receives or generates sample ions from a solvent matrix and removes at least some of the solvent matrix from the sample ions. A separation chamber downstream of the desolvation region has a separation chamber inlet communicating with the desolvation region, for receiving the desolvated sample ions along with non-ionised solvent and solvent ion vapours. The separation chamber has electrodes for generating an electric field within the separation chamber, defining a first flow path for sample ions between the separation chamber inlet and a separation chamber outlet. Unwanted solvent ions and non-ionised solvent vapours are directed away from the separation chamber outlet. The sample introduction system has a reaction chamber with an inlet communicating with the separation chamber outlet, for receiving the sample ions from the separation chamber and for decomposing the received ions into smaller products.
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公开(公告)号：US10665438B2
公开(公告)日：2020-05-26
申请号：US15260622
申请日：2016-09-09
Applicant: Thermo Fisher Scientific (Bremen) GmbH
Inventor： Lothar Rottmann , Alexander Makarov , Hans-Juergen Schlueter , Christoph Wehe
Abstract: An elemental mass spectrometer uses a mass filter to select ions from ions received from an ion source and transmit the selected ions. A reaction or collision cell receives the transmitted ions and reacts or collides these with a gas to provide product ions thereby. A mass analyzer receives the product ions, analyzes them and provides at least one output based on detection of the analyzed ions. The elemental mass spectrometer is operated to provide a first output from the mass analyzer measuring ions within a first analysis range of mass-to-charge ratios including a desired mass-to-charge ratio, M, to provide a second output from the mass analyzer measuring ions within a second analysis range of mass-to-charge ratios including a mass-to-charge ratio at least 0.95 atomic mass units lower than the desired mass-to-charge ratio, (M−i), i≥0.95 and to correct the first output on the basis of the second output.
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公开(公告)号：US10600625B2
公开(公告)日：2020-03-24
申请号：US15675125
申请日：2017-08-11
Applicant: Thermo Fisher Scientific (Bremen) GmbH
Inventor： Norbert Quaas , Hans-Juergen Schlueter , Gerhard Jung
Abstract: A method of calibrating a mass spectrometer is disclosed. The mass spectrometer includes a first quadrupole, a second mass analyzer and a detection means. The method includes calibrating the second mass analyzer at a first time, calibrating the first quadrupole at a second time later than the first including a) determining for each of several selected masses a corresponding value of the amplitude of the RF voltage and DC voltage applied to the electrodes of the first quadrupole, b) fitting a function of the selected mass to the values of the amplitude of the RF voltage and DC voltage corresponding to the several selected masses, c) detecting the selected mass in a filter window width over a mass range, d) evaluating a shift of the peak position and/or a deviation of the filter window width, and e) repeating the calibration steps under certain conditions.
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公开(公告)号：US10446380B2
公开(公告)日：2019-10-15
申请号：US16130760
申请日：2018-09-13
Applicant: Thermo Fisher Scientific (Bremen) GmbH
Inventor： Alexander A. Makarov , Stevan R. Horning
Abstract: A sample introduction system for a spectrometer comprises a desolvation region that receives or generates sample ions from a solvent matrix and removes at least some of the solvent matrix from the sample ions. A separation chamber downstream of the desolvation region has a separation chamber inlet communicating with the desolvation region, for receiving the desolvated sample ions along with non-ionized solvent and solvent ion vapors. The separation chamber has electrodes for generating an electric field within the separation chamber, defining a first flow path for sample ions between the separation chamber inlet and a separation chamber outlet. Unwanted solvent ions and non-ionized solvent vapors are directed away from the separation chamber outlet. The sample introduction system has a reaction chamber with an inlet communicating with the separation chamber outlet, for receiving the sample ions from the separation chamber and for decomposing the received ions into smaller products.
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58.发明申请
公开(公告)号：US20190244798A1
公开(公告)日：2019-08-08
申请号：US16385372
申请日：2019-04-16
Inventor： Alexander MAKAROV , Oleg BOYARKINE , Vladimir KOPYSOV
IPC: H01J49/00
CPC classification number: H01J49/0059 , H01J49/0031
Abstract: A method of analyzing molecules, comprising: generating ions from a sample of molecules; cooling the generated ions below ambient temperature; fragmenting at least some of the cooled ions by irradiating the ions with light at a plurality of different wavelengths (λ) within one or more predetermined spectral intervals; recording a fragment mass spectrum of the fragmented ions comprising a detected signal (I) versus m/z over a predetermined range of m/z values for each of the plurality of different wavelengths (λ), thereby recording a two-dimensional dependency of the detected signal (I) on m/z and irradiation wavelength (λ); and determining from the recorded two-dimensional dependency an identity of at least one of the generated ions and/or relative abundances of different generated ions and thereby determining an identity of at least of one of the molecules and/or relative abundances of different molecules in the sample.
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公开(公告)号：US20190221416A1
公开(公告)日：2019-07-18
申请号：US16292815
申请日：2019-03-05
Applicant: Thermo Fisher Scientific (Bremen) GmbH
Inventor： Johannes SCHWIETERS , Henning WEHRS
CPC classification number: H01J49/105 , H01J49/0027
Abstract: Methods of determining isobaric interference during mass analysis in a mass spectrometer are provided. The methods comprise comparing interference-free reaction profiles of a chemical species to reaction profiles of the same chemical species that may comprise isobaric interference, wherein a determination of a difference between the profiles is an indication of isobaric interference being present. Methods of quantifying isobaric interference are also provided, including methods of correcting isotope ratios determined in the presence of isobaric interference.
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公开(公告)号：US10309941B2
公开(公告)日：2019-06-04
申请号：US15684762
申请日：2017-08-23
Applicant: Thermo Fisher Scientific (Bremen) GmbH
Inventor： Eric Wapelhorst , Hans-Jürgen Schlüter , Oliver Kracht , Johannes Schwieters , Michael Krummen
Abstract: A gas inlet system for introducing gas into an isotope ratio analyzer, the gas inlet system including a reference system comprising: a first supply of a reference gas having a first known isotope ratio; a supply of a carrier gas, wherein the supplies of reference gas and carrier gas are each connected by respective reference and carrier gas lines to a first mixing junction where the reference gas and carrier gas combine; a mixing zone connected downstream of the first mixing junction wherein the combined reference gas and carrier gas mix together; an exit line for transporting the mixed gas from the mixing zone to the isotope ratio analyzer; and an opening on the exit line, wherein the opening is downstream of the mixing zone. Also provided is a method of determining an isotope ratio.
