公开(公告)号：US5225533A

公开(公告)日：1993-07-06

申请号：US673682

申请日：1991-03-22

申请人： William J. Rutter ,  Daniel V. Santi

发明人： William J. Rutter ,  Daniel V. Santi

IPC分类号： C07K1/04 ,  C07K1/36 ,  G01N33/68

CPC分类号： G01N33/68 ,  C07K1/047 ,  C07K1/36

摘要： A method to obtain selected individual peptides or families thereof which have a target property and optionally to determine the amino acid sequence of a selected peptide or peptides to permit synthesis in practical quantities is disclosed. In general outline, the method of the invention comprises synthesizing a mixture of randomly or deliberately generated peptides using standard synthesis techniques, but adjusting the individual concentrations of the components of a mixture of sequentially added amino acids according to the coupling constants for each amino acid/amino acid coupling. The subgroup of peptides having the target property can then be selected, and either each peptide isolated and sequenced, or analysis performed on the mixture to permit its composition to be reproduced. Also included in the invention is an efficient method to determine the relevant coupling constants.

摘要翻译： 公开了获得具有目标特性并任选地确定所选择的肽或肽的氨基酸序列以允许实际合成的所选单独肽或其家族的方法。 总的来说，本发明的方法包括使用标准合成技术合成随机或故意产生的肽的混合物，但是根据每个氨基酸/偶联物的偶联常数调节相继添加的氨基酸的混合物的组分的个体浓度， 氨基酸偶联。 然后可以选择具有靶特性的肽亚组，并且分离和测序每个肽，或对混合物进行分析以允许其复制其组成。 本发明还包括确定相关耦合常数的有效方法。

公开(公告)号：US5164490A

公开(公告)日：1992-11-17

申请号：US437511

申请日：1989-11-15

申请人： Daniel V. Santi ,  Jeffrey Edman ,  Ursula Edman

发明人： Daniel V. Santi ,  Jeffrey Edman ,  Ursula Edman

IPC分类号： C12N1/21 ,  C12N9/06

CPC分类号： C12N9/003 ,  C12N9/0046

摘要： Genetic material encoding dihydrofolate reductase (DHFR) of Pneumocystis carinii has been isolated and characterized. This genetic material allows the production of peptides for use in the design of inhibitors specific for P. carinii DHFR (as opposed to DHFR in the host infected with P. carinii) or can itself be directly used in hybridization assays.

摘要翻译： 已经分离和表征了编码卡氏肺囊虫的二氢叶酸还原酶（DHFR）的遗传物质。 这种遗传物质允许生产肽用于设计对卡氏肺囊虫DHFR特异性的抑制剂（与在卡氏肺囊虫感染的宿主中的DHFR相反）或本身可以直接用于杂交测定。