公开(公告)号：US20060141494A1

公开(公告)日：2006-06-29

申请号：US11209702

申请日：2005-08-24

申请人： Hideki Kambara ,  Tomoharu Kajiyama ,  Kunio Harada ,  Masao Kamahori

发明人： Hideki Kambara ,  Tomoharu Kajiyama ,  Kunio Harada ,  Masao Kamahori

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： G01N21/03 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/76

摘要： An object of the present invention is to provide a luminescence detection apparatus compact in size which is capable of conveniently determining DNA base sequences at a low cost. According to the present invention, a luminescence detection apparatus 1 is provided comprising: a plurality of reaction cells 6 each having a transparent bottom portion; a solution-dispensing portion 19 equipped with capillaries 18 positioned above the reaction cells 6 and put into a one-to-one correspondence with the reaction cells 6; and a light-detecting portion 29 having a plurality of light-sensing elements 24 put into a one-to-one correspondence with the reaction cells 6 and arranged in proximity to the bottom surfaces of the reaction cells 6, wherein the a plurality of light-sensing elements 24 of the light-detecting portion 29 detect respective luminescences in the reaction cells 6 generated by injecting reagent solutions from the solution-dispensing portion 19 to the reaction cells 6.

摘要翻译： 本发明的目的是提供一种尺寸紧凑的发光检测装置，其能够以低成本方便地确定DNA碱基序列。 根据本发明，提供一种发光检测装置1，其包括：多个反应池6，每个反应池6具有透明底部; 溶液分配部分19，其配备有位于反应单元6上方并与反应单元6一一对应的毛细管18; 以及具有多个光检测元件24的光检测部分29，其与反应单元6一一对应地布置在反应单元6的底表面附近，其中多个光 光检测部分29的光敏元件24检测通过从溶液分配部分19将反应池6注入试剂溶液而产生的反应池6中的相应发光。

公开(公告)号：US20050100943A1

公开(公告)日：2005-05-12

申请号：US10964602

申请日：2004-10-12

申请人： Hideki Kambara ,  Masato Mitsuhashi

发明人： Hideki Kambara ,  Masato Mitsuhashi

IPC分类号： B01J19/00 ,  B05D3/00 ,  C12M1/34 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/543

CPC分类号： B01L3/5027 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00308 ,  B01J2219/00337 ,  B01J2219/00364 ,  B01J2219/00367 ,  B01J2219/00405 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L2200/0631 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0816 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/54313

摘要： The use of probe arrays in which probes of various biological substances such as DNA are immobilized on the surface of a solid is becoming established as an effective means for high-speed screening. Different kinds of probes, such as DNA, are immobilized on the surface of a multiple number of independently treatable fine particles, such as beads, instead of the surface of a single solid, and the resulting beads are aligned in a capillary or a cell in a designated order. The size of the area where one probe is immobilized is reduced. The bead probe array is characterized in that such small beads are aligned one by one in a designated manner using a sheet with holes, and one or a multiple number of beads are held in the holes and then transferred to a probe array holder such as a capillary.

摘要翻译： 使用其中各种生物物质如DNA的探针固定在固体表面上的探针阵列正在成为高速筛选的有效手段。 将不同种类的探针（例如DNA）固定在多个可独立处理的细颗粒（例如珠粒）的表面上而不是单个固体的表面上，并将所得的珠粒在毛细管或细胞中排列 指定的订单。 一个探针被固定的区域的尺寸减小了。 珠探针阵列的特征在于，使用具有孔的片以指定的方式一个接一个地排列这样的小珠，并且将一个或多个珠保持在孔中，然后转移到探针阵列保持器，例如 毛细管。

公开(公告)号：US06576108B1

公开(公告)日：2003-06-10

申请号：US09524269

申请日：2000-03-13

申请人： Satoshi Takahashi ,  Hideki Kambara

发明人： Satoshi Takahashi ,  Hideki Kambara

IPC分类号： G01N2726

CPC分类号： G01N27/44782 ,  G01N27/44721 ,  G01N2035/00237

摘要： An electrophoresis apparatus includes an optical cell having an outlet for fluid and filled with a buffer solution, and a plurality of electrophoresis lanes, each including a separation medium, which separate samples labeled with fluorophores, one end of each of the electrophoresis lanes being disposed in the optical cell to enable the separated samples labeled with the fluorophores to migrate from the electrophoresis lanes into the buffer solution in the optical cell. There is also provided a vessel containing a buffer solution, the vessel being disposed so that a level of the buffer solution in the vessel is higher than the ends of the electrophoresis lanes disposed in the optical cell, a passage connecting the vessel with the optical cell, a light source which emits light which excites the fluorophores to emit fluorescence, and a photodetector which detects the fluorescence emitted by the fluorophores.

摘要翻译： 电泳装置包括具有流体出口并填充有缓冲溶液的光学单元和多个电泳通道，每个电泳通道均包含分离介质，分离介质分离出用荧光团标记的样品，每个电泳通道的一端设置在 光电池使得用荧光团标记的分离样品从电泳泳道迁移到光学池中的缓冲溶液中。 还提供了一种容纳缓冲溶液的容器，该容器被设置成使得容器中的缓冲溶液的水平高于设置在光学单元中的电泳通道的端部，连接容器与光学单元的通道 发出激发荧光团发出荧光的光的光源，以及检测由荧光团发出的荧光的光检测器。

公开(公告)号：US06527933B1

公开(公告)日：2003-03-04

申请号：US09666047

申请日：2000-09-20

申请人： Hideki Kambara

发明人： Hideki Kambara

IPC分类号： G01N27453

CPC分类号： G01N27/44743 ,  G01N27/44726

摘要： The DNA sample preparation apparatus of the present invention comprises a base plate having a plurality of first grooves for fixing two or more kinds of DNA samples or primers to the inner surfaces of the grooves, respectively, a second groove communicating with the plurality of the first grooves, wherein a reaction solution is introduced into the first grooves to be reacted with the two or more kinds of said DNA samples or primers independently at the same time.

摘要翻译： 本发明的DNA样品制备装置包括：具有多个用于将两种或更多种DNA样品或引物固定在所述槽的内表面的多个第一凹槽的基板，与所述多个第一 沟槽，其中将反应溶液引入第一沟槽中以与两种或更多种所述DNA样品或引物同时反应。

公开(公告)号：US06518027B2

公开(公告)日：2003-02-11

申请号：US09793098

申请日：2001-02-27

申请人： Hideki Kambara

发明人： Hideki Kambara

IPC分类号： C12Q168

CPC分类号： B01L3/50851 ,  B01L7/52

摘要： A sample preparation apparatus for DNA analysis comprises a holder for separating specific primers on the basis of size, color, weight, dimension, or degree of magnetization, the specific primers having base sequences complementary to a plurality of DNA fragments to be amplified via PCR, and the specific primers being capable of binding respectively to the DNA fragments; and a reaction-solution-holding plate having a concavity which accommodates one edge of the holder and a PCR solution containing a common primer capable of hybridizing with the base sequence of an oligonucleotide introduced into the 5′-end of each of the DNA fragments, and the DNA fragments. The PCR amplification of the DNA fragments is carried out by using the specific primers (immobilized on the surfaces of a plurality of mutually separable supports with respect to each DNA fragments) and the common primer (a mobile primer common to all DNA fragments) to produce PCR amplification products inside the corresponding portions of the holder. The DNA fragments are derived from a plurality of DNAs to be amplified by PCR under the same conditions at the same time to avoid undesired mutual interference among the primers, and the PCR products can be separated and recovered for each of the DNA fragments. The sample preparation method for DNA analysis comprises the relevant amplifying, separating and recovering steps as described above.

公开(公告)号：US6054035A

公开(公告)日：2000-04-25

申请号：US897833

申请日：1997-07-21

申请人： Hideki Kambara

发明人： Hideki Kambara

IPC分类号： G01N33/53 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  G01N1/28 ,  G01N27/447 ,  G01N33/566 ,  G01N37/00 ,  G01N27/26

CPC分类号： G01N27/44743 ,  G01N27/44726

摘要： The DNA sample preparation apparatus of the present invention comprises a base plate having a plurality of first grooves for fixing two or more kinds of DNA samples or primers to the inner surfaces of the grooves, respectively, a second groove communicating with the plurality of the first grooves, wherein a reaction solution is introduced into the first grooves to be reacted with the two or more kinds of said DNA samples or primers independently at the same time.

公开(公告)号：US5964998A

公开(公告)日：1999-10-12

申请号：US931276

申请日：1997-09-16

申请人： Hideki Kambara

发明人： Hideki Kambara

IPC分类号： G01N27/447 ,  G01N35/00 ,  G01N27/26

CPC分类号： G01N27/44721 ,  G01N27/44782 ,  G01N2035/00237

摘要： An electrophoresis apparatus for detecting samples migrating in migration portions includes a plurality of gel separation portions separate from one another and having respective ends disposed along a straight line, a plurality of migration portions equal in number to the gel separation portions and disposed along a straight line following respective ones of the gel separation portions, a light source for generating light which passes through the migration portions, the light propagating along a straight line extending through all of the migration portions, a plurality of optical fibers having respective first ends and respective second ends, the first ends of the optical fibers being disposed facing respective points where the light from the light source passes through respective ones of the migration portions, the optical fibers being equal in number to or greater in number than the migration portions, and an optical detector optically coupled to the second ends of the optical fibers for receiving light from the migration portions via the optical fibers.

摘要翻译： 用于检测在迁移部分迁移的样品的电泳装置包括多个彼此分离的凝胶分离部分，并且具有沿着直线设置的各个端部，多个迁移部分数量等于凝胶分离部分并沿着直线 按照各个凝胶分离部分，产生通过迁移部分的光的光源，沿着穿过所有迁移部分的直线传播的光，具有各自的第一端和相应的第二端的多根光纤 光纤的第一端面对来自光源的光通过相应的迁移部分的光纤的数量相等或更大的各个点，以及光检测器 光学耦合到光纤的第二端 用于经由光纤从迁移部分接收光。

公开(公告)号：US5954932A

公开(公告)日：1999-09-21

申请号：US88849

申请日：1998-06-02

申请人： Satoshi Takahashi ,  Hideki Kambara

发明人： Satoshi Takahashi ,  Hideki Kambara

IPC分类号： G01N27/447 ,  G01N35/00 ,  G01N27/26

CPC分类号： G01N27/44782 ,  G01N27/44721 ,  G01N2035/00237

摘要： A method for analyzing samples includes forming a plurality of sheath flows of a buffer solution, each of the sheath flows being formed at one end of a respective one of a plurality of capillaries each containing a separation medium, causing samples to migrate through the capillaries into the sheath flows, and detecting the samples in the sheath flows.

摘要翻译： 用于分析样品的方法包括形成缓冲溶液的多个护套流，每个护套流动形成在多个毛细管中的相应一个毛细管的一端，每个毛细管包含分离介质，使样品迁移通过毛细管进入 护套流动，并检测护套流中的样品。

公开(公告)号：US5833827A

公开(公告)日：1998-11-10

申请号：US710894

申请日：1996-09-23

申请人： Takashi Anazawa ,  Satoshi Takahashi ,  Hideki Kambara

发明人： Takashi Anazawa ,  Satoshi Takahashi ,  Hideki Kambara

IPC分类号： G01N27/447 ,  G01N35/00 ,  G01N27/26

CPC分类号： G01N27/44721 ,  G01N27/44782 ,  G01N2035/00237

摘要： The capillary array electrophoresis system of the present invention comprises a plurality of capillaries whose inside is filled with migration medium and at least parts of which are aligned in a plane, a means for making lasers substantially simultaneously irradiate transparent parts of the plurality of capillaries along the direction of alignment of the plurality of capillaries so as to excite fluorephore labels of migrated and separated samples, a fluorescence detection means for detecting fluorescence radiating from the fluorephore labels, from a direction crossing the direction of the alignment, the transparent parts of the plurality of capillaries are surrounded by a transparent gas, the cross section of the capillary of the transparent part is a circle and the ratio of the outside diameter to the inside diameter of the capillary ranges from 1 to 7.

摘要翻译： 本发明的毛细管阵列电泳系统包括多个毛细管，其内部填充有迁移介质，并且其至少一部分在平面中对准，用于使激光器基本上同时沿着多个毛细管的透明部分照射的装置沿着 多个毛细管的排列方向，以激发迁移和分离的样品的荧光标记;荧光检测装置，用于检测从荧光标记物发出的荧光，从与取向方向相交的方向，多个 毛细管被透明气体包围，透明部分的毛细管的横截面为圆形，毛细管的外径与内径之比为1〜7。

公开(公告)号：US5759374A

公开(公告)日：1998-06-02

申请号：US669915

申请日：1996-06-25

申请人： Satoshi Takahashi ,  Hideki Kambara

发明人： Satoshi Takahashi ,  Hideki Kambara

IPC分类号： G01N27/447 ,  G01N35/00 ,  G01N27/26

CPC分类号： G01N27/44782 ,  G01N27/44721 ,  G01N2035/00237

摘要： An electrophoresis apparatus includes a plurality of first capillaries containing a separation medium, such as a gel, and an optical cell in which one end of each of the first capillaries is disposed. Samples labeled with fluorophores and introduced into the first capillaries, and an electric field is applied to the first capillaries to cause the samples to migrate through the first capillaries into the optical cell. A light source excites the fluorophores with light when the samples are in the optical cell, causing the fluorophores to emit fluorescence, and a photo-detecting system detects the fluorescence emitted by the fluorophores. The ends of the first capillaries in the optical cell may be arranged in a straight line. The light source may include a He--Ne laser emitting light having a wavelength of 594 nm, and the fluorophores may include either sulforhodamine or a derivative of sulforhodamine. The apparatus may include a plurality of second capillaries each having one end disposed in the optical cell such that the ends of the second capillaries are separated from the ends of the first capillaries by respective gaps, or may include a single second capillary have one end disposed in the optical cell such that the end of the single second capillary is separated from the ends of the first capillaries. A sheath solution may be introduced into the optical cell to form sheath flows of the sheath solution around the ends of the first capillaries.

摘要翻译： 电泳装置包括多个包含分离介质（例如凝胶）的第一毛细管和其中设置每个第一毛细管的一端的光学单元。 用荧光团标记并引入第一毛细管的样品，并且将电场施加到第一毛细管，使得样品通过第一毛细管迁移到光学细胞中。 当样品在光学单元中时，光源用光激发荧光团，导致荧光团发出荧光，光检测系统检测荧光团发出的荧光。 光学单元中的第一毛细管的端部可以布置成直线。 光源可以包括发射波长为594nm的He-Ne激光，并且荧光团可以包括磺酰罗丹明或磺酰罗丹明的衍生物。 该装置可以包括多个第二毛细管，每个具有设置在光学单元中的一端，使得第二毛细管的端部通过相应的间隙与第一毛细管的端部分开，或者可以包括单个第二毛细管，其一端设置 在光学单元中使得单个第二毛细管的端部与第一毛细管的端部分离。 可以将鞘溶液引入到光学电池中，以在第一毛细管的端部周围形成护套溶液的护套流。