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113 条记录 (耗时 0.041 秒)

    Nucleotide sequences for encoding of the lysR2-gene
    51.
    发明授权
    Nucleotide sequences for encoding of the lysR2-gene 失效
    编码lysR2基因的核苷酸序列

    公开(公告)号：US07078502B2

    公开(公告)日：2006-07-18

    申请号：US09826909

    申请日：2001-04-06

    申请人： Bettina Moeckel ,  Mike Farwick ,  Thomas Hermann ,  Caroline Kreutzer ,  Walter Pfefferle

    发明人： Bettina Moeckel ,  Mike Farwick ,  Thomas Hermann ,  Caroline Kreutzer ,  Walter Pfefferle

    IPC分类号： C07H21/02 ,  C07H21/04 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63

    CPC分类号： C12P13/08 ,  C07K14/34

    摘要： Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.

    摘要翻译： 分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸，b）多核苷酸， 其编码多肽，其包含与SEQ ID No.2的氨基酸序列至少70％的程度相同的氨基酸序列，c）与a）或b）的多核苷酸互补的多核苷酸， 和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少lysR2基因存在于减毒 形式，以及使用多核苷酸序列作为杂交探针。

    Nucleotide sequences encoding the ptsH gene
    52.
    发明授权
    Nucleotide sequences encoding the ptsH gene 有权
    编码ptsH基因的核苷酸序列

    公开(公告)号：US06818432B2

    公开(公告)日：2004-11-16

    申请号：US09819930

    申请日：2001-03-29

    申请人： Mike Farwick ,  Bettina Mockel ,  Walter Pfefferle

    发明人： Mike Farwick ,  Bettina Mockel ,  Walter Pfefferle

    IPC分类号： C12N120

    CPC分类号： C12N9/1205 ,  C12P13/04 ,  C12P13/08

    摘要： The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group comprising a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no.2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids with enhancement of the ptsH gene coding for component H of the phosphotransferase system, and the use of the above polynucleotides as primer or hybridisation probe.

    摘要翻译： 本发明涉及含有多核苷酸序列的分离的多核苷酸，所述多核苷酸序列选自包含a）多核苷酸的多核苷酸，所述多核苷酸与编码含有SEQ ID NO：1的氨基酸序列的多肽的多核苷酸具有至少70％的同一性。 2，b）编码含有与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽的多核苷酸，c）与a）或b）的多核苷酸互补的多核苷酸， 和d）多核苷酸，其包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸，以及用于增强编码磷酸转移酶系统的组分H的ptsH基因的L-氨基酸的发酵生产的方法， 以及使用上述多核苷酸作为引物或杂交探针。

    Process for the preparation of L-amino acids by attenuating the mikE17 gene
    53.
    发明授权
    Process for the preparation of L-amino acids by attenuating the mikE17 gene 有权
    通过减弱mikE17基因制备L-氨基酸的方法

    公开(公告)号：US07202061B2

    公开(公告)日：2007-04-10

    申请号：US10923726

    申请日：2004-08-24

    申请人： Mike Farwick ,  Klaus Hutchmacher ,  Walter Pfefferle

    发明人： Mike Farwick ,  Klaus Hutchmacher ,  Walter Pfefferle

    IPC分类号： C12P13/04 ,  C12N1/20 ,  C12N15/00 ,  C07K1/00 ,  C07J21/04

    CPC分类号： C12P13/08 ,  C07K14/34

    摘要： The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the mikE17 gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    摘要翻译： 本发明涉及一种分离的多核苷酸，其包含选自组内的多核苷酸序列

    Nucleotide sequences for encoding of the lysR2-gene
    54.
    发明申请
    Nucleotide sequences for encoding of the lysR2-gene 失效
    编码lysR2基因的核苷酸序列

    公开(公告)号：US20050282259A1

    公开(公告)日：2005-12-22

    申请号：US11178485

    申请日：2005-07-12

    申请人： Bettina Moeckel ,  Mike Farwick ,  Thomas Hermann ,  Caroline Kreutzer ,  Walter Pfefferle

    发明人： Bettina Moeckel ,  Mike Farwick ,  Thomas Hermann ,  Caroline Kreutzer ,  Walter Pfefferle

    IPC分类号： C07K14/34 ,  C12N1/21 ,  C12N15/31 ,  C12P13/08

    CPC分类号： C12P13/08 ,  C07K14/34

    摘要： Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.

    摘要翻译： 分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸，b）多核苷酸， 其编码多肽，其包含与SEQ ID No.2的氨基酸序列至少70％的程度相同的氨基酸序列，c）与a）或b）的多核苷酸互补的多核苷酸， 和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少lysR2基因存在于减毒 形式，以及使用多核苷酸序列作为杂交探针。

    Nucleotide sequences coding for the citA gene
    56.
    发明申请
    Nucleotide sequences coding for the citA gene 有权
    编码citA基因的核苷酸序列

    公开(公告)号：US20050112664A1

    公开(公告)日：2005-05-26

    申请号：US10977474

    申请日：2004-11-01

    申请人： Bettina Mockel ,  Mike Farwick ,  Thomas Hermann ,  Achim Marx ,  Walter Pfefferle

    发明人： Bettina Mockel ,  Mike Farwick ,  Thomas Hermann ,  Achim Marx ,  Walter Pfefferle

    IPC分类号： A61K38/48 ,  C07K14/34 ,  C12N9/12 ,  C12P13/08 ,  C12Q1/68 ,  C07H21/04 ,  C12N1/21 ,  C12N9/10 ,  C12N15/74

    CPC分类号： C12N9/1205 ,  C07K14/34 ,  C12N9/10 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12P13/222 ,  C12P13/225 ,  C12P13/227 ,  C12P13/24

    摘要： The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which. comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the citA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    摘要翻译： 本发明涉及分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同至少70％ ，b）编码多肽的多核苷酸。 包括与SEQ ID No.2的氨基酸序列至少70％的程度相同的氨基酸序列，c）与a）或b）的多核苷酸互补的多核苷酸，和d）包含在 a），b）或c）的多核苷酸序列的至少15个连续核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少citA基因以减毒形式存在，以及使用 包含根据本发明的序列的多核苷酸作为杂交探针。

    Process for the fermentative preparation of L-amino acids using coryneform bacteria
    58.
    发明授权
    Process for the fermentative preparation of L-amino acids using coryneform bacteria 失效
    使用棒状细菌发酵制备L-氨基酸的方法

    公开(公告)号：US06596516B2

    公开(公告)日：2003-07-22

    申请号：US09731826

    申请日：2000-12-08

    申请人： Petra Ziegler ,  Lothar Eggeling ,  Hermann Sahm ,  Georg Thierbach ,  Walter Pfefferle

    发明人： Petra Ziegler ,  Lothar Eggeling ,  Hermann Sahm ,  Georg Thierbach ,  Walter Pfefferle

    IPC分类号： C12P1304

    CPC分类号： C12N9/1014 ,  C12P13/04 ,  C12P13/08

    摘要： A process for the preparation of L-amino acids, in which the following steps are carried out, a) fermenting the desired L-amino acid-producing bacteria in which at least the glyA gene is attenuated, in particular by removal of the natural promoter, and optionally b) concentrating the desired product in the medium or in the cells of the bacteria and c) isolating the L-amino acid, and optionally bacteria in which further genes of the biosynthesis pathway of the desired L-amino acid are additionally amplified are employed, or bacteria in which the metabolic pathways which reduce the formation of the desired L-amino acid are at least partly eliminated are employed, and nucleotide sequences of the lacI-tac-5′glyA or lacI-tac-glyA unit.

    摘要翻译： 一种制备L-氨基酸的方法，其中进行以下步骤：a）将至少所述glyA基因减毒的所需L-氨基酸产生细菌发酵，特别是通过除去天然启动子 并且任选地b）将所需产物浓缩在细菌的培养基中或细胞中，并且c）分离L-氨基酸，以及任选的细菌，其中另外扩增所需L-氨基酸的生物合成途径的其它基因的细菌 或其中代谢途径减少所需L-氨基酸的形成至少部分消除的细菌，以及lacI-tac-5'glyA或lacI-tac-glyA单元的核苷酸序列。