公开(公告)号：US06716641B1

公开(公告)日：2004-04-06

申请号：US09856484

申请日：2001-09-14

申请人： Erling Sundrehagen

发明人： Erling Sundrehagen

IPC分类号： G01N3353

CPC分类号： G01N33/558 ,  G01N33/68 ,  G01N33/98 ,  G01N2333/79 ,  G01N2800/307 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/973 ,  Y10S435/975 ,  Y10S436/808 ,  Y10T436/25375 ,  Y10T436/255

摘要： The invention relates to a new dipstick assay for detecting and quantifying the content of an target analyte in a sample. The assay is particularly useful for example in the diagnosis and monitoring of alcoholism by the detection of asialo transferrin or carbohydrate free transferrin (CFT). Thus, provided is a dipstick for determining the content of a target analyte variant in a mixture of analyte variants in a sample, comprising: a) a sample application zone, b) a screening zone having an immobilised binding ligand having a binding affinity for a non-target analyte variant or varinats, c) a conjugate zone comprising a detector reagent, d) a reading zone for detection of said analyte.

摘要翻译： 本发明涉及一种用于检测和定量样品中目标分析物的含量的新的测试仪测定。 该测定法通过检测脱唾液酸转铁蛋白或不含碳水化合物的转铁蛋白（CFT）来诊断和监测酒精中毒特别有用。 因此，提供了一种用于确定样品中分析物变体混合物中目标分析物变体的含量的量尺，包括：a）样品施用区，b）具有固定的结合配体的筛选区，其具有对 非目标分析物变体或变体，c）包含检测试剂的共轭区，d）用于检测所述分析物的读取区。

公开(公告)号：US6103478A

公开(公告)日：2000-08-15

申请号：US26156

申请日：1998-02-19

申请人： Erling Sundrehagen

发明人： Erling Sundrehagen

IPC分类号： G01N33/48 ,  G01N33/53 ,  G01N33/68 ,  G01N33/84 ,  G01N33/98

CPC分类号： G01N33/6893 ,  G01N33/68 ,  G01N33/84 ,  G01N33/98 ,  G01N2333/79 ,  G01N2800/307 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/973 ,  Y10S435/975 ,  Y10S436/808 ,  Y10T436/25375 ,  Y10T436/255

摘要： The invention provides a method of assessment of carbohydrate-deficient transferrin in a transferrin containing body fluid, said method comprising the steps of:i) obtaining a transferrin containing liquid sample of or derived from a said fluid;ii) contacting said sample with a source of iron ions;iii) subsequently contacting said sample with an anionic ion exchange resin at a pH such as to cause carbohydrate-deficient transferrin to be retained by said resin;iv) subsequently contacting said resin with an eluant serving to release carbohydrate-deficient transferrin into the eluate from said resin;v) collecting a volume of said eluate substantially free from tetra- and penta-sialo transferrin; andvi) assessing the transferrin variant content in said volume of eluate.By including at least a proportion of the trisialotransferrin in the eluate, it is possible to use relatively simple assessment techniques such as turbidimetry in the assay.

摘要翻译： 本发明提供了一种评价含运载运蛋白的体液中的碳水化合物缺乏型运铁蛋白的方法，所述方法包括以下步骤：i）获得含有液体样品或来自所述流体的液体样品; ii）使所述样品与铁离子源接触; iii）随后使所述样品与阴离子交换树脂接触，所述阴离子交换树脂在pH下使得所述树脂保留碳水化合物缺乏的转铁蛋白; iv）随后使所述树脂与用于将碳水化合物缺乏的运铁蛋白从所述树脂中分离到洗脱液中的洗脱剂接触; v）收集基本上不含四 - 和五 - 唾液酸转铁蛋白的体积的所述洗脱液; 和vi）评估所述体积的洗脱液中的转铁蛋白变体含量。 通过在洗脱液中包含至少一部分三铁转铁蛋白，可以使用相对简单的评估技术，例如测定中的比浊法。

公开(公告)号：US6054322A

公开(公告)日：2000-04-25

申请号：US737894

申请日：1997-02-28

申请人： Pekka Sillanaukee ,  Kaija Seppa ,  Ola M.ang.rtensson

发明人： Pekka Sillanaukee ,  Kaija Seppa ,  Ola M.ang.rtensson

IPC分类号： G01N33/92 ,  C12Q1/34 ,  G01N33/98 ,  G01N33/48 ,  G01N33/49 ,  G01N33/493

CPC分类号： C12Q1/34 ,  G01N33/98 ,  G01N2800/307 ,  Y10T436/142222 ,  Y10T436/143333 ,  Y10T436/145555 ,  Y10T436/201666 ,  Y10T436/203332 ,  Y10T436/204165

摘要： A method for the determination of an individual's alcohol consumption, characterized in comprising the steps: i) determining the level of sialic acid in a body fluid sample from the individual; ii) comparing the level found in step (i) with the level for the normal population, an increased level being an indication of an increased alcohol consumption relative to the consumption (per individual) of the normal population.

摘要翻译： PCT No.PCT / SE95 / 00672 371日期1997年2月23日 102（e）日期1997年2月23日PCT提交1995年6月7日PCT公布。 WO95 / 33993 PCT出版物 日期1995年12月14日一种用于确定个体饮酒量的方法，其特征在于包括以下步骤：i）确定来自个体的体液样品中唾液酸的水平; ii）将步骤（i）中发现的水平与正常人口的水平进行比较，增加的水平是相对于正常人群的消费（每个人）增加的饮酒量的指示。

公开(公告)号：US5834626A

公开(公告)日：1998-11-10

申请号：US758557

申请日：1996-11-29

申请人： Emory S. De Castro ,  Douglas R. Malat

发明人： Emory S. De Castro ,  Douglas R. Malat

IPC分类号： G01N21/78 ,  G01N31/22 ,  G01N33/497 ,  G01N33/98 ,  G01N27/62 ,  G01N1/22

CPC分类号： G01N33/98 ,  G01N21/783 ,  G01N31/22 ,  G01N33/4972 ,  G01N2800/307

摘要： Colorimetric indicators for breath, air, gas, & vapor analyses that can be thrown away after a single use, are easy to use, low cost, small, and simple to manufacture for indicating at least volume passed through the colorimetric indicators and may also be used to indicate concentration of an analyte within breath, air, gas, & vapor passed through the colorimetric indicators. The present invention is directed to colorimetric indicators for breath, air, gas, & vapor analyses. The colorimetric indicators may have a housing with an entrance port, an outlet, a volume indicator, and an analyte indicating reagent. The volume indicator may be a breath volume reagent. Different analytes may be measured, the analyte indicating reagent depending upon the particular analyte being to monitored. The breath volume indicator is colorimetric, and changes color when a predetermined volume of air is passed through the colorimetric indicators for breath, air, gas, & vapor analyses. The analyte indicator reagent is also colorimetric, and changes color when a predetermined amount of chemical concentration of the analyte is exposed to the analyte indicator reagent. A process for manufacturing a colorimetric indicator for breath, air, gas, and vapor analyses, comprises at least the following steps: immobilizing a reagent, such color change inducing reagent being composed of at least one constant concentration component.

摘要翻译： 用于呼吸，空气，气体和蒸汽分析的比色指示剂，可以在一次使用后丢弃，易于使用，成本低，制造简单，用于指示至少体积通过比色指示剂，并且还可以 用于指示呼吸中的分析物浓度，空气，气体和蒸气通过比色指示剂。 本发明涉及用于呼吸，空气，气体和蒸气分析的比色指示剂。 比色指示器可以具有带入口，出口，体积指示器和分析物指示试剂的外壳。 体积指示器可以是呼吸容积试剂。 可以测量不同的分析物，分析物指示试剂取决于待监测的特定分析物。 呼吸量指示器是比色的，并且当预定体积的空气通过用于呼吸，空气，气体和蒸气分析的比色指示剂时，颜色变化。 分析物指示剂试剂也是比色的，并且当预定量的分析物的化学浓度暴露于分析物指示剂试剂时，颜色变化。 用于制造用于呼吸，空气，气体和蒸气分析的比色指示剂的方法至少包括以下步骤：固定试剂，所述试剂由至少一种恒定浓度组分组成。

公开(公告)号：US5798212A

公开(公告)日：1998-08-25

申请号：US716913

申请日：1996-09-09

申请人： Erling Sundrehagen

发明人： Erling Sundrehagen

IPC分类号： G01N33/566 ,  G01N33/68 ,  G01N33/84 ,  G01N33/53 ,  G01N33/543 ,  G01N33/563

CPC分类号： G01N33/84 ,  G01N33/566 ,  G01N33/6893 ,  G01N2333/79 ,  G01N2800/307 ,  Y10S435/962 ,  Y10S435/973 ,  Y10S435/975 ,  Y10S436/808 ,  Y10T436/25375 ,  Y10T436/255

摘要： The invention provides a method of assessment of carbohydrate-deficient transferrin in a transferrin containing body fluid, said method comprising the steps of: i) obtaining a transferrin containing liquid sample of or derived from a said fluid; ii) contacting said sample with a source of iron ions; iii) subsequently contacting said sample with an anionic ion exchange resin at a pH such as to cause carbohydrate-deficient transferrin to be retained by said resin; iv) subsequently contacting said resin with an eluant serving to release carbohydrate-deficient transferrin into the eluate from said resin; v) collecting a volume of said eluate substantially free from tetra- and penta-sialo transferrin; and vi) assessing the transferrin variant content in said volume of eluate. By including at least a proportion of the trisialotransferrin in the eluate, it is possible to use relatively simple assessment techniques such as turbidimetry in the assay.

公开(公告)号：US4900664A

公开(公告)日：1990-02-13

申请号：US914326

申请日：1986-10-02

申请人： Yedy Israel ,  Ruth Arnon

发明人： Yedy Israel ,  Ruth Arnon

IPC分类号： G01N33/531 ,  G01N33/68 ,  G01N33/72 ,  G01N33/98

CPC分类号： G01N33/98 ,  G01N33/531 ,  G01N33/68 ,  G01N33/721 ,  G01N2800/307

摘要： A method is provided for detecting and measuring acetaldehyde-protein condensates. An antibody is produced to an antigen, the antibody being cross-reactive with acetaldehyde-protein condensates, these condensates including protein moieties corresponding to or different from the protein moiety of the antigen. Detection and measurement of acetaldehyde-protein condensates may be performed by reacting the antibody with the acetaldehyde-protein condensate to form a complex and measuring the complex.

摘要翻译： 提供了一种用于检测和测量乙醛 - 蛋白质冷凝物的方法。 对抗原产生抗体，抗体与乙醛 - 蛋白质缩合物交叉反应，这些缩合物包括对应于或不同于抗原蛋白部分的蛋白质部分。 乙醛 - 蛋白质缩合物的检测和测量可以通过使抗体与乙醛 - 蛋白质缩合物反应以形成复合物并测量该复合物来进行。